|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 4 497-504, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
D Cao, G Lin, EM Westphale, EC Beyer and TH Steinberg
Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA.
Insulin-mediated increases in cytosolic calcium are synchronized among the cells in a pancreatic islet, and result in pulsatile secretion of insulin. Pancreatic beta cells express the gap junction protein connexin43 and are functionally coupled, making gap junctional communication a likely mechanism for the synchronization of calcium transients among islet cells. To define the mechanism by which pancreatic islet cells coordinate calcium responses, we studied mechanically-induced intercellular calcium waves in the communication-deficient rat insulinoma cell line RINm5f, and in RINm5f cells transfected with the gap junction protein connexin43. Both RINm5f and RINm5f cells transfected with connexin43 propagated calcium waves that required release of calcium from intracellular stores, did not involve gap junctional communication, and appeared to be mediated by autocrine activity of secreted ATP acting on P2U purinergic receptors. Connexin43 transfectants also propagated calcium waves that required gap junctional communication and influx of extracellular calcium through voltage-gated calcium channels. Gap junction-dependent intercellular calcium waves were inhibited by preventing plasma membrane depolarization. These studies demonstrate two distinct pathways by which insulin-secreting cells can coordinate cytosolic calcium rises, and show that it is by ionic traffic that gap junctions synchronize calcium-dependent events in these cells.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Tabata, Y. Takemura, M. Kobayashi, M. Ikeda, S. Nishimura, Y. Sato, R. Tatsumi, Y. Ikeuchi, and H. Iwamoto Mechanism of Ca2+ increase in myoblasts derived from chicken embryos J. Electron Microsc. (Tokyo), October 1, 2006; 55(5): 265 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Furuya, M. Sokabe, and S. Furuya Characteristics of subepithelial fibroblasts as a mechano-sensor in the intestine: cell-shape-dependent ATP release and P2Y1 signaling J. Cell Sci., August 1, 2005; 118(15): 3289 - 3304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Salehi, S. S. Qader, E. Grapengiesser, and B. Hellman Inhibition of Purinoceptors Amplifies Glucose-Stimulated Insulin Release With Removal of its Pulsatility Diabetes, July 1, 2005; 54(7): 2126 - 2131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Schaefer, H. Mischak, S. Schnell, A. Griese, R. Iakubov, G. Riepenhausen, and C. Schofl Mechanisms of Arginine-Vasopressin-Induced Ca2+ Oscillations in {beta}-Cells (HIT-T15): A Role for Oscillating Protein Kinase C Endocrinology, October 1, 2004; 145(10): 4635 - 4644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. V. Rocheleau, G. M. Walker, W. S. Head, O. P. McGuinness, and D. W. Piston Microfluidic glucose stimulation reveals limited coordination of intracellular Ca2+ activity oscillations in pancreatic islets PNAS, August 31, 2004; 101(35): 12899 - 12903. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zarkovic and J.-C. Henquin Synchronization and entrainment of cytoplasmic Ca2+ oscillations in cell clusters prepared from single or multiple mouse pancreatic islets Am J Physiol Endocrinol Metab, August 1, 2004; 287(2): E340 - E347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Hellman, H. Dansk, and E. Grapengiesser Pancreatic {beta}-cells communicate via intermittent release of ATP Am J Physiol Endocrinol Metab, May 1, 2004; 286(5): E759 - E765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Lin, J. K. Rurangirwa, M. Koval, and T. H. Steinberg Gap junctional communication modulates agonist-induced calcium oscillations in transfected HeLa cells J. Cell Sci., February 22, 2004; 117(6): 881 - 887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Lundquist, P. Alm, A. Salehi, R. Henningsson, E. Grapengiesser, and B. Hellman Carbon monoxide stimulates insulin release and propagates Ca2+ signals between pancreatic {beta}-cells Am J Physiol Endocrinol Metab, November 1, 2003; 285(5): E1055 - E1063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. K. Nihei, A. C. Campos de Carvalho, D. C. Spray, W. Savino, and L. A. Alves A novel form of cellular communication among thymic epithelial cells: intercellular calcium wave propagation Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1304 - C1313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yao, M. Suwa, B. Li, K. Kawamura, T. Morioka, and T. Oite ATP-Dependent Mechanism for Coordination of Intercellular Ca2+ Signaling and Renin Secretion in Rat Juxtaglomerular Cells Circ. Res., August 22, 2003; 93(4): 338 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Caton, A. Calabrese, C. Mas, V. Serre-Beinier, A. Charollais, D. Caille, R. Zufferey, D. Trono, and P. Meda Lentivirus-mediated transduction of connexin cDNAs shows level- and isoform-specific alterations in insulin secretion of primary pancreatic {beta}-cells J. Cell Sci., June 1, 2003; 116(11): 2285 - 2294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Leite, E. C. Thrower, W. Echevarria, P. Koulen, K. Hirata, A. M. Bennett, B. E. Ehrlich, and M. H. Nathanson Nuclear and cytosolic calcium are regulated independently PNAS, March 4, 2003; 100(5): 2975 - 2980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Koval Sharing signals: connecting lung epithelial cells with gap junction channels Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L875 - L893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yamagata, T. Nammo, M. Moriwaki, A. Ihara, K. Iizuka, Q. Yang, T. Satoh, M. Li, R. Uenaka, K. Okita, et al. Overexpression of Dominant-Negative Mutant Hepatocyte Nuclear Factor-1{alpha} in Pancreatic {beta}-Cells Causes Abnormal Islet Architecture With Decreased Expression of E-Cadherin, Reduced {beta}-cell Proliferation, and Diabetes Diabetes, January 1, 2002; 51(1): 114 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. E. Isakson, W. H. Evans, and S. Boitano Intercellular Ca2+ signaling in alveolar epithelial cells through gap junctions and by extracellular ATP Am J Physiol Lung Cell Mol Physiol, February 1, 2001; 280(2): L221 - L228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. E. Klepeis, A. Cornell-Bell, and V. Trinkaus-Randall Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells J. Cell Sci., January 12, 2001; 114(23): 4185 - 4195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. O. Suadicani, M. J. Vink, and D. C. Spray Slow intercellular Ca2+ signaling in wild-type and Cx43-null neonatal mouse cardiac myocytes Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H3076 - H3088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Berthoud, E. C. Beyer, and K. H. Seul Peptide inhibitors of intercellular communication Am J Physiol Lung Cell Mol Physiol, October 1, 2000; 279(4): L619 - L622. [Full Text] [PDF]
|
|
|
|
|
|
S. Boitano and W. H. Evans Connexin mimetic peptides reversibly inhibit Ca2+ signaling through gap junctions in airway cells Am J Physiol Lung Cell Mol Physiol, October 1, 2000; 279(4): L623 - L630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Homolya, T. H. Steinberg, and R. C. Boucher Cell to Cell Communication in Response to Mechanical Stress via Bilateral Release of Atp and Utp in Polarized Epithelia J. Cell Biol., September 18, 2000; 150(6): 1349 - 1360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kimura, K. Suzuki, T. Sagara, T. Nishida, T. Yamamoto, and Y. Kitazawa Regulation of Connexin Phosphorylation and Cell-Cell Coupling in Trabecular Meshwork Cells Invest. Ophthalmol. Vis. Sci., July 1, 2000; 41(8): 2222 - 2228. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. Cirelli, P. Lebrun, C. Gueuning, A. Moens, J. Delogne-Desnoeck, C. Dictus-Vermeulen, A.-M. Vanbellinghen, and S. Meuris Secretory characteristics and viability of human term placental tissue after overnight cold preservation Hum. Reprod., April 1, 2000; 15(4): 756 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. B. Guthrie, J. Knappenberger, M. Segal, M. V. L. Bennett, A. C. Charles, and S. B. Kater ATP Released from Astrocytes Mediates Glial Calcium Waves J. Neurosci., January 15, 1999; 19(2): 520 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Cotrina, J. H.-C. Lin, and M. Nedergaard Cytoskeletal Assembly and ATP Release Regulate Astrocytic Calcium Signaling J. Neurosci., November 1, 1998; 18(21): 8794 - 8804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Guan, B. F. Cravatt, G. R. Ehring, J. E. Hall, D. L. Boger, R. A. Lerner, and N. B. Gilula The Sleep-inducing Lipid Oleamide Deconvolutes Gap Junction Communication and Calcium Wave Transmission in Glial Cells J. Cell Biol., December 29, 1997; 139(7): 1785 - 1792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Jorgensen, S. T. Geist, R. Civitelli, and T. H. Steinberg ATP- and Gap Junction-dependent Intercellular Calcium Signaling in Osteoblastic Cells J. Cell Biol., October 20, 1997; 139(2): 497 - 506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Vozzi, D. Bosco, E. Dupont, A. Charollais, and P. Meda Hyperinsulinemia-Induced Hypoglycemia Is Enhanced by Overexpression of Connexin 43 Endocrinology, July 1, 1997; 138(7): 2879 - 2885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wagner, O. Oliyarnyk, W. Gartner, P. Nowotny, M. Groeger, K. Kaserer, W. Waldhausl, and M. S. Pasternack Cloning and Expression of Secretagogin, a Novel Neuroendocrine- and Pancreatic Islet of Langerhans-specific Ca2+-binding Protein J. Biol. Chem., August 4, 2000; 275(32): 24740 - 24751. [Abstract] [Full Text] [PDF]
|
|
