|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 9 1051-1062, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
A Kohler, MS Schmidt-Zachmann and WW Franke
Division of Cell Biology, German Cancer Research Center, Heidelberg, Germany.
Using a specific monoclonal antibody (mAb AND-1/23-5-14) we have identified, cDNA-cloned and characterized a novel DNA-binding protein of the clawed toad, Xenopus laevis, that is accumulated in the nucleoplasm of oocytes and various other cells. This protein comprises 1,127 amino acids, with a total molecular mass of 125 kDa and a pI of 5.27. It is encoded by a mRNA of approximately 4 kb and contains, in addition to clusters of acidic amino acids, two hallmark motifs: the amino-terminal part harbours seven consecutive 'WD-repeats', which are sequence motifs of about 40 amino acids that are characteristic of a large group of regulatory proteins involved in diverse cellular functions, while the carboxy terminal portion possesses a 63-amino-acid-long 'HMG-box', which is typical of a family of DNA-binding proteins involved in regulation of chromatin assembly, transcription and replication. The DNA-binding capability of the protein was demonstrated by DNA affinity chromatography and electrophoretic mobility shift assays using four-way junction DNA. Protein AND-1 (acidic nucleoplasmic DNA-binding protein) appears as an oligomer, probably a homodimer, and has been localized throughout the entire interchromatinic space of the interphase nucleoplasm, whereas during mitosis it is transiently dispersed over the cytoplasm. We also identified a closely related, perhaps orthologous protein in mammals. The unique features of protein AND-1, which is a 'natural chimera' combining properties of the WD-repeat and the HMG-box families of proteins, are discussed in relation to its possible nuclear functions.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Jonkers and M. Rep Lessons from Fungal F-Box Proteins Eukaryot. Cell, May 1, 2009; 8(5): 677 - 695. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Farina, J.-H. Shin, D.-H. Kim, V. P. Bermudez, Z. Kelman, Y.-S. Seo, and J. Hurwitz Studies with the Human Cohesin Establishment Factor, ChlR1: ASSOCIATION OF ChlR1 WITH Ctf18-RFC AND Fen1 J. Biol. Chem., July 25, 2008; 283(30): 20925 - 20936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zhu, C. Ukomadu, S. Jha, T. Senga, S. K. Dhar, J. A. Wohlschlegel, L. K. Nutt, S. Kornbluth, and A. Dutta Mcm10 and And-1/CTF4 recruit DNA polymerase {alpha} to chromatin for initiation of DNA replication Genes & Dev., September 15, 2007; 21(18): 2288 - 2299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zick, I. Onn, R. Bezalel, H. Margalit, and J. Shlomai Assigning functions to genes: identification of S-phase expressed genes in Leishmania major based on post-transcriptional control elements Nucleic Acids Res., July 28, 2005; 33(13): 4235 - 4242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Gygax, C. P. Semighini, G. H. Goldman, and S. D. Harris SepBCTF4 Is Required for the Formation of DNA-Damage-Induced UvsCRAD51 Foci in Aspergillus nidulans Genetics, March 1, 2005; 169(3): 1391 - 1402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Williams and J. R. McIntosh Mcl1p Is a Polymerase {alpha} Replication Accessory Factor Important for S-Phase DNA Damage Survival Eukaryot. Cell, January 1, 2005; 4(1): 166 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. P. Bermudez, Y. Maniwa, I. Tappin, K. Ozato, K. Yokomori, and J. Hurwitz The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA PNAS, September 2, 2003; 100(18): 10237 - 10242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Williams and J. R. McIntosh mcl1+, the Schizosaccharomyces pombe Homologue of CTF4, Is Important for Chromosome Replication, Cohesion, and Segregation Eukaryot. Cell, October 1, 2002; 1(5): 758 - 773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Zirwes, J. Eilbracht, S. Kneissel, and M. S. Schmidt-Zachmann A Novel Helicase-Type Protein in the Nucleolus: Protein NOH61 Mol. Biol. Cell, April 1, 2000; 11(4): 1153 - 1167. [Abstract] [Full Text]
|
|
|
|
|
|
T. Formosa and T. Nittis Dna2 Mutants Reveal Interactions with Dna Polymerase {alpha} and Ctf4, a Pol {alpha} Accessory Factor, and Show That Full Dna2 Helicase Activity Is Not Essential for Growth Genetics, April 1, 1999; 151(4): 1459 - 1470. [Abstract] [Full Text]
|
|
|
|
|
|
R. F. Zirwes, M. S. Schmidt-Zachmann, and W. W. Franke Identification of a small, very acidic constitutive nucleolar protein (NO29) as a member of the nucleoplasmin family PNAS, October 14, 1997; 94(21): 11387 - 11392. [Abstract] [Full Text] [PDF]
|
|
