|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 1 71-79, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
A Merdes and DW Cleveland
Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla, CA 92093-0660, USA.
NuMA is an essential protein for the formation of spindle poles in mitosis. During interphase, NuMA is transported into the nucleus where it resides until prometaphase of the next mitotic cycle. We tested for a potential function of NuMA in interphase nuclei that were assembled from human sperm DNA using frog egg extract immunodepleted of NuMA. Despite the absence of NuMA, nuclei formed without visible changes of the chromatin structure, surrounded by an intact nuclear membrane containing pores and nuclear lamins. These nuclei were fully competent to import nuclear substrates and to replicate their DNA. By screening tissue sections of various organs, absence of NuMA from the nucleus was observed in a number of cell types, including sperm, granulocytes in the blood, and differentiated smooth and skeletal muscle fibers. Experiments on cultured myoblasts indicated that NuMA is degraded during muscle cell differentiation. The absence of NuMA in interphase nuclei of the tissues tested correlated with a non-spherical, elongated or beaded nuclear morphology, suggesting that during interphase NuMA may act as a non-essential nucleoskeletal element.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. D. Silk, A. J. Holland, and D. W. Cleveland Requirements for NuMA in maintenance and establishment of mammalian spindle poles J. Cell Biol., March 9, 2009; 184(5): 677 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Si, S. C. Verma, M. A. Lampson, Q. Cai, and E. S. Robertson Kaposi's Sarcoma-Associated Herpesvirus-Encoded LANA Can Interact with the Nuclear Mitotic Apparatus Protein To Regulate Genome Maintenance and Segregation J. Virol., July 1, 2008; 82(13): 6734 - 6746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yamauchi, K. Kiriyama, H. Kimura, and Y. Nishiyama Herpes simplex virus induces extensive modification and dynamic relocalisation of the nuclear mitotic apparatus (NuMA) protein in interphase cells J. Cell Sci., June 15, 2008; 121(12): 2087 - 2096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C. Abad, J. Lewis, I. S. Mian, D. W. Knowles, J. Sturgis, S. Badve, J. Xie, and S. A. Lelievre NuMA Influences Higher Order Chromatin Organization in Human Mammary Epithelium Mol. Biol. Cell, February 1, 2007; 18(2): 348 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-Y. Yan, J.-C. Huang, Z.-Y. Zhu, Z.-L. Lei, L.-H. Shi, C.-L. Nan, Z.-J. Zhao, Y.-C. OuYang, X.-F. Song, Q.-Y. Sun, et al. NuMA distribution and microtubule configuration in rabbit oocytes and cloned embryos. Reproduction, December 1, 2006; 132(6): 869 - 876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Dai, L. Wang, H. Wang, Y. Liu, N. Li, Q. Lyu, D. L Keefe, D. F Albertini, and L. Liu Fate of centrosomes following somatic cell nuclear transfer (SCNT) in bovine oocytes. Reproduction, June 1, 2006; 131(6): 1051 - 1061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Knowles, D. Sudar, C. Bator-Kelly, M. J. Bissell, and S. A. Lelievre Automated local bright feature image analysis of nuclear protein distribution identifies changes in tissue phenotype PNAS, March 21, 2006; 103(12): 4445 - 4450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Mariappan and V. K. Parnaik Sequestration of pRb by Cyclin D3 Causes Intranuclear Reorganization of Lamin A/C during Muscle Cell Differentiation Mol. Biol. Cell, April 1, 2005; 16(4): 1948 - 1960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Taimen and M. Kallajoki NuMA and nuclear lamins behave differently in Fas-mediated apoptosis J. Cell Sci., February 1, 2003; 116(3): 571 - 583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Krauss, R. Heald, G. Lee, W. Nunomura, J. A. Gimm, N. Mohandas, and J. A. Chasis Two Distinct Domains of Protein 4.1 Critical for Assembly of Functional Nuclei in Vitro J. Biol. Chem., November 8, 2002; 277(46): 44339 - 44346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Bh. Muralikrishna, J. Dhawan, N. Rangaraj, and V. K. Parnaik Distinct changes in intranuclear lamin A/C organization during myoblast differentiation J. Cell Sci., March 13, 2002; 114(22): 4001 - 4011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wittmann, M. Wilm, E. Karsenti, and I. Vernos Tpx2, a Novel Xenopus Map Involved in Spindle Pole Organization J. Cell Biol., June 26, 2000; 149(7): 1405 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Kosova, N. Pante, C. Rollenhagen, A. Podtelejnikov, M. Mann, U. Aebi, and E. Hurt Mlp2p, A Component of Nuclear Pore Attached Intranuclear Filaments, Associates with Nic96p J. Biol. Chem., January 7, 2000; 275(1): 343 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Fry, P Descombes, C Twomey, R Bacchieri, and E. Nigg The NIMA-related kinase X-Nek2B is required for efficient assembly of the zygotic centrosome in Xenopus laevis J. Cell Sci., January 6, 2000; 113(11): 1973 - 1984. [Abstract] [PDF]
|
|
|
|
|
|
S Sazer and M Dasso The ran decathlon: multiple roles of Ran J. Cell Sci., January 4, 2000; 113(7): 1111 - 1118. [Abstract] [PDF]
|
|
|
|
 |
|
 K. Ye, D. A. Compton, M. M. Lai, L. D. Walensky, and S. H. Snyder Protein 4.1N Binding to Nuclear Mitotic Apparatus Protein in PC12 Cells Mediates the Antiproliferative Actions of Nerve Growth Factor J. Neurosci., December 15, 1999; 19(24): 10747 - 10756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Melnick and J. D. Licht Deconstructing a Disease: RAR{alpha}, Its Fusion Partners, and Their Roles in the Pathogenesis of Acute Promyelocytic Leukemia Blood, May 15, 1999; 93(10): 3167 - 3215. [Full Text] [PDF]
|
|
|
|
|
|
S. N. Mattagajasingh, S.-C. Huang, J. S. Hartenstein, M. Snyder, V. T. Marchesi, and E. J. Benz Jr. A Nonerythroid Isoform of Protein 4.1R Interacts with the Nuclear Mitotic Apparatus (NuMA) Protein J. Cell Biol., April 5, 1999; 145(1): 29 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Lelievre, V. M. Weaver, J. A. Nickerson, C. A. Larabell, A. Bhaumik, O. W. Petersen, and M. J. Bissell Tissue phenotype depends on reciprocal interactions between the extracellular matrix and the structural organization of the nucleus PNAS, December 8, 1998; 95(25): 14711 - 14716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-M. Tassin, C. Celati, M. Moudjou, and M. Bornens Characterization of the Human Homologue of the Yeast Spc98p and Its Association with {gamma}-Tubulin J. Cell Biol., May 4, 1998; 141(3): 689 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. Parker, R. M. Sandoval, H. A. Feister, J. P. Bidwell, and S. J. Rhodes The Homeodomain Coordinates Nuclear Entry of the Lhx3 Neuroendocrine Transcription Factor and Association with the Nuclear Matrix J. Biol. Chem., July 28, 2000; 275(31): 23891 - 23898. [Abstract] [Full Text] [PDF]
|
|
