|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 10 1363-1370, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
M Meyer-Ficca, J Muller-Navia and H Scherthan
Abt. fur Humanbiologie, Universitat Kaiserslautern, Postf. 3049, D-67653 Kaiserslautern, Germany.
Fluorescence in situ hybridization with centromeric, telomeric and whole chromosome paint probes was used to study nuclear topology in epididymal sperm as well as spermatids from testis tissue sections of the rat. Pericentromeric regions of 9 chromosomes of the rat (n=21) were labeled with a satellite I specific DNA probe. Pericentromeres showed few tandem associations in spermatids of steps 1-8 of spermiogenesis. At step 9, pericentromeric regions associated to form an elongated cluster in the spermatid nucleus. This arrangement was also seen in the sperm nucleus. FISH with telomere probes revealed numerous, variably arranged signals in round and elongated spermatids as well as sperm nuclei. Telomere signals showed a tendency for pairwise association which was more pronounced in elongated spermatid and epididymal sperm nuclei. FISH to DTT treated sperm suggested that telomeres reside at the periphery and that pericentromeres are located in the nuclear interior. Chromosome painting with rat chromosome 2 and 12 specific microdissection library probes showed that these chromosomes predominantly occupy compact and variably shaped territories during spermatid maturation. In elongated epididymal sperm nuclei chromosome 2 and 12 territories took up specific positions. We suppose that the associations of pericentromeres during step 9 render a well defined nuclear topology which facilitates the ordered compaction of the genome at subsequent stages.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  K.A. Finch, K.G.L. Fonseka, A. Abogrein, D. Ioannou, A.H. Handyside, A.R. Thornhill, N. Hickson, and D.K. Griffin Nuclear organization in human sperm: preliminary evidence for altered sex chromosome centromere position in infertile males Hum. Reprod., June 1, 2008; 23(6): 1263 - 1270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. K. Greaves, D. Rangasamy, M. Devoy, J. A. Marshall Graves, and D. J. Tremethick The X and Y Chromosomes Assemble into H2A.Z, Containing Facultative Heterochromatin, following Meiosis. Mol. Cell. Biol., July 1, 2006; 26(14): 5394 - 5405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Mudrak, N. Tomilin, and A. Zalensky Chromosome architecture in the decondensing human sperm nucleus J. Cell Sci., October 1, 2005; 118(19): 4541 - 4550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Foster, L. R. Abeydeera, D. K. Griffin, and J. M. Bridger Non-random chromosome positioning in mammalian sperm nuclei, with migration of the sex chromosomes during late spermatogenesis J. Cell Sci., May 1, 2005; 118(9): 1811 - 1820. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Martianov, S. Brancorsini, R. Catena, A. Gansmuller, N. Kotaja, M. Parvinen, P. Sassone-Corsi, and I. Davidson Polar nuclear localization of H1T2, a histone H1 variant, required for spermatid elongation and DNA condensation during spermiogenesis PNAS, February 22, 2005; 102(8): 2808 - 2813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Martianov, S. Brancorsini, A. Gansmuller, M. Parvinen, I. Davidson, and P. Sassone-Corsi Distinct functions of TBP and TLF/TRF2 during spermatogenesis: requirement of TLF for heterochromatic chromocenter formation in haploid round spermatids Development, March 4, 2003; 129(4): 945 - 955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Pasierbek, M. Jantsch, M. Melcher, A. Schleiffer, D. Schweizer, and J. Loidl A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome pairing and disjunction Genes & Dev., June 1, 2001; 15(11): 1349 - 1360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Gineitis, I. A. Zalenskaya, P. M. Yau, E. M. Bradbury, and A. O. Zalensky Human Sperm Telomere-binding Complex Involves Histone H2B and Secures Telomere Membrane Attachment J. Cell Biol., December 27, 2000; 151(7): 1591 - 1598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. O'Carroll, H. Scherthan, A. H. F. M. Peters, S. Opravil, A. R. Haynes, G. Laible, S. Rea, M. Schmid, A. Lebersorger, M. Jerratsch, et al. Isolation and Characterization of Suv39h2, a Second Histone H3 Methyltransferase Gene That Displays Testis-Specific Expression Mol. Cell. Biol., December 15, 2000; 20(24): 9423 - 9433. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Scherthan, M. Jerratsch, B. Li, S. Smith, M. Hultén, T. Lock, and T. de Lange Mammalian Meiotic Telomeres: Protein Composition and Redistribution in Relation to Nuclear Pores Mol. Biol. Cell, December 1, 2000; 11(12): 4189 - 4203. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Kozik, E. M. Bradbury, and A. O. Zalensky Identification and Characterization of a Bovine Sperm Protein That Binds Specifically to Single-Stranded Telomeric Deoxyribonucleic Acid Biol Reprod, February 1, 2000; 62(2): 340 - 346. [Abstract] [Full Text]
|
|
