spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koch, A. W.
Right arrow Articles by David, C. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koch, A. W.
Right arrow Articles by David, C. N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 111, Issue 11 1545-1554, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

Spinalin, a new glycine- and histidine-rich protein in spines of Hydra nematocysts

AW Koch, TW Holstein, C Mala, E Kurz, J Engel and CN David
Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, Switzerland.

Here we present the cloning, expression and immunocytochemical localization of a novel 24 kDa protein, designated spinalin, which is present in the spines and operculum of Hydra nematocysts. Spinalin cDNA clones were identified by in situ hybridization to differentiating nematocytes. Sequencing of a full-length clone revealed the presence of an N-terminal signal peptide, suggesting that the mature protein is sorted via the endoplasmic reticulum to the post-Golgi vacuole in which the nematocyst is formed. The N-terminal region of spinalin (154 residues) is very rich in glycines (48 residues) and histidines (33 residues). A central region of 35 residues contains 19 glycines, occurring mainly as pairs. For both regions a polyglycine-like structure is likely and this may be stabilized by hydrogen bond-mediated chain association. Similar sequences found in loricrins, cytokeratins and avian keratins are postulated to participate in formation of supramolecular structures. Spinalin is terminated by a basic region (6 lysines out of 15 residues) and an acidic region (9 glutamates and 9 aspartates out of 32 residues). Western blot analysis with a polyclonal antibody generated against a recombinant 19 kDa fragment of spinalin showed that spinalin is localized in nematocysts. Following dissociation of the nematocyst's capsule wall with DTT, spinalin was found in the insoluble fraction containing spines and the operculum. Immunocytochemical analysis of developing nematocysts revealed that spinalin first appears in the matrix but then is transferred through the capsule wall at the end of morphogenesis to form spines on the external surface of the inverted tubule and the operculum.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Biol. Bull.Home page
A. J. Reft, J. A. Westfall, and D. G. Fautin
Formation of the Apical Flaps in Nematocysts of Sea Anemones (Cnidaria: Actiniaria)
Biol. Bull., August 1, 2009; 217(1): 25 - 34.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
J. S. Hwang, Y. Takaku, J. Chapman, K. Ikeo, C. N. David, and T. Gojobori
Cilium Evolution: Identification of a Novel Protein, Nematocilin, in the Mechanosensory Cilium of Hydra Nematocytes
Mol. Biol. Evol., September 1, 2008; 25(9): 2009 - 2017.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. Krucken, R. J. Hosse, A. N. Mouafo, R. Entzeroth, S. Bierbaum, P. Marinovski, K. Hain, G. Greif, and F. Wunderlich
Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56
Eukaryot. Cell, February 1, 2008; 7(2): 202 - 211.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. S. Hwang, H. Ohyanagi, S. Hayakawa, N. Osato, C. Nishimiya-Fujisawa, K. Ikeo, C. N. David, T. Fujisawa, and T. Gojobori
The evolutionary emergence of cell type-specific genes inferred from the gene expression analysis of Hydra
PNAS, September 11, 2007; 104(37): 14735 - 14740.
[Abstract] [Full Text] [PDF]

Home page
 J R Soc InterfaceHome page
C. C Broomell, R. K Khan, D. N Moses, A. Miserez, M. G Pontin, G. D Stucky, F. W Zok, and J. H. Waite
Mineral minimization in nature's alternative teeth
J R Soc Interface, February 22, 2007; 4(12): 19 - 31.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
U. Engel, S. Oezbek, R. Engel, B. Petri, F. Lottspeich, and T. W. Holstein
Nowa, a novel protein with minicollagen Cys-rich domains, is involved in nematocyst formation in Hydra
J. Cell Sci., October 15, 2002; 115(20): 3923 - 3934.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Szczepanek, M. Cikala, and C. N. David
Poly-{gamma}-glutamate synthesis during formation of nematocyst capsules in Hydra
J. Cell Sci., February 15, 2002; 115(4): 745 - 751.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R Deutzmann, S Fowler, X Zhang, K Boone, S Dexter, R. Boot-Handford, R Rachel, and M. Sarras
Molecular, biochemical and functional analysis of a novel and developmentally important fibrillar collagen (Hcol-I) in hydra
Development, January 11, 2000; 127(21): 4669 - 4680.
[Abstract] [PDF]


© The Company of Biologists Ltd 1998