|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 12 1729-1739, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
N Guillen, P Boquet and P Sansonetti
Unite de Pathogenie Microbienne Moleculaire, Institut National de la Sante et de la Recherche Medicale U389 Institut Pasteur, France. nguillen@pasteur.fr
Entamoeba histolytica is a protozoan parasite that invades human intestine leading to ulceration and destruction of tissue. Amoebic movement and phagocytosis of human cells is accompanied by characteristic changes in cell morphology. Amoebae become polarized, developing a frontal pseudopod and a well-defined rear zone of membrane accumulation designated the uroid. In motile eukaryotic cells, a phenomenon that contributes to movement is the capping of receptors at the cell surface. During the capping process, E. histolytica concentrates ligand-receptor complexes in the uroid. Interestingly, some of these surface receptors are involved in the survival of the parasite. While looking for regulators of capping and uroid formation, we identified RacG, an E. histolytica protein that is homologous to human Rac1. This protein belongs to the Rac subfamily of small GTPases implicated in interactions between the actin cytoskeleton and the membrane of mammalian cells. Cloning of the EhracG gene and analysis of the protein activity either in murine fibroblasts or in E. histolytica revealed that EhRacG induces a characteristic Rac phenotype. When expressed in amoebae, an EhRacG-V12 mutant protein not only deregulated cell polarity, but also caused a defect in cytokinesis. Analysis of the cytoskeleton in amoebae bearing this mutant revealed that F-actin concentrated at the periphery of the cell. In addition, the number and localization of uroids were modified. These results suggest a role for EhRacG in amoebic morphogenesis and cytokinesis.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Majumder and A. Lohia Entamoeba histolytica Encodes Unique Formins, a Subset of Which Regulates DNA Content and Cell Division Infect. Immun., June 1, 2008; 76(6): 2368 - 2378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Majumder, G. Schmidt, A. Lohia, and K. Aktories EhRho1, a RhoA-Like GTPase of Entamoeba histolytica, Is Modified by Clostridial Glucosylating Cytotoxins Appl. Envir. Microbiol., December 1, 2006; 72(12): 7842 - 7848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Okada, C. D. Huston, B. J. Mann, W. A. Petri Jr., K. Kita, and T. Nozaki Proteomic Analysis of Phagocytosis in the Enteric Protozoan Parasite Entamoeba histolytica Eukaryot. Cell, April 1, 2005; 4(4): 827 - 831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Saito-Nakano, T. Yasuda, K. Nakada-Tsukui, M. Leippe, and T. Nozaki Rab5-associated Vacuoles Play a Unique Role in Phagocytosis of the Enteric Protozoan Parasite Entamoeba histolytica J. Biol. Chem., November 19, 2004; 279(47): 49497 - 49507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kumagai, A. Makioka, T. Takeuchi, and T. Nozaki Molecular Cloning and Characterization of a Protein Farnesyltransferase from the Enteric Protozoan Parasite Entamoeba histolytica J. Biol. Chem., January 16, 2004; 279(3): 2316 - 2323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Labruyere, C. Zimmer, V. Galy, J.-C. Olivo-Marin, and N. Guillen EhPAK, a member of the p21-activated kinase family, is involved in the control of Entamoeba histolytica migration and phagocytosis J. Cell Sci., January 1, 2003; 116(1): 61 - 71. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hirose, T. Kawashima, I. Iwamoto, T. Nosaka, and T. Kitamura MgcRacGAP Is Involved in Cytokinesis through Associating with Mitotic Spindle and Midbody J. Biol. Chem., February 16, 2001; 276(8): 5821 - 5828. [Abstract] [Full Text] [PDF]
|
|
