|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 12 1741-1749, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
MA van der Heyden, MB Rook, MM Hermans, G Rijksen, J Boonstra, LH Defize and OH Destree
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, The Netherlands.
Wnt mediated signal transduction is considered to regulate activity of target genes. In Xenopus embryos, ectopic Wnt1 and Wnt8 expression induces gap-junctional communication. During murine brain formation, Wnt1 and the gap-junctional protein connexin43 (Cx43) are co-expressed at the mid/hindbrain border, while interference with Wnt1 or Cx43 expression during embryogenesis leads to severe brain defects in the mid/hindbrain region. In PC12 cells, Wnt1 expression leads to an apparent increase in cell-cell adhesion. We investigated the effects of Wnt1 overexpression on gap-junctional communication in PC12 cells. Wnt1 expressing clones displayed an increased electrical and chemical coupling. This coincides with an increased expression of Cx43 mRNA and protein, while other connexins, Cx26, Cx32, Cx37, Cx40 and Cx45, were not up-regulated. Also, induction of Wnt1 expression in a mammary epithelial cell line leads to an increase in gap-junctional communication and Cx43 protein expression. In transient transactivation assays in P19 EC cells we found that Wnt1 and Li+, an ion that mimics Wnt signalling, increased transcription from the rat Cx43 promoter, potentially via TCF/LEF binding elements, in a pathway separate from cAMP-induced Cx43 transactivation. The results demonstrate that Cx43 acts as a functional target of Wnt1 signalling, and Cx43 expression can be regulated by Wnt1 at the transcriptional level. Our data suggest that Wnt1-induced cell fate determination is likely to involve regulation of gap-junctional communication.
This article has been cited by other articles:
|
|
 |
|
  X.-q. Gan, J.-y. Wang, Y. Xi, Z.-l. Wu, Y.-p. Li, and L. Li Nuclear Dvl, c-Jun, {beta}-catenin, and TCF form a complex leading to stabiLization of {beta}-catenin-TCF interaction J. Cell Biol., March 24, 2008; 180(6): 1087 - 1100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Saaf, J. M. Halbleib, X. Chen, S. T. Yuen, S. Y. Leung, W. J. Nelson, and P. O. Brown Parallels between Global Transcriptional Programs of Polarizing Caco-2 Intestinal Epithelial Cells In Vitro and Gene Expression Programs in Normal Colon and Colon Cancer Mol. Biol. Cell, November 1, 2007; 18(11): 4245 - 4260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Mak, H. L. Kenerson, L. D. Aicher, E. A. Barnes, and R. S. Yeung Aberrant {beta}-Catenin Signaling in Tuberous Sclerosis Am. J. Pathol., July 1, 2005; 167(1): 107 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. T. Bommer, C. Jager, E.-M. Durr, S. Baehs, S. T. Eichhorst, T. Brabletz, G. Hu, T. Frohlich, G. Arnold, D. C. Kress, et al. DRO1, a Gene Down-regulated by Oncogenes, Mediates Growth Inhibition in Colon and Pancreatic Cancer Cells J. Biol. Chem., March 4, 2005; 280(9): 7962 - 7975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. E.J. Teunissen, H. J. Jongsma, and M. F.A. Bierhuizen Regulation of myocardial connexins during hypertrophic remodelling Eur. Heart J., November 2, 2004; 25(22): 1979 - 1989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. N. Everly Jr., S. Kusano, and N. Raab-Traub Accumulation of Cytoplasmic {beta}-Catenin and Nuclear Glycogen Synthase Kinase 3{beta} in Epstein-Barr Virus-Infected Cells J. Virol., November 1, 2004; 78(21): 11648 - 11655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. King and P. D. Lampe Mice deficient for the gap junction protein Connexin32 exhibit increased radiation-induced tumorigenesis associated with elevated mitogen-activated protein kinase (p44/Erk1, p42/Erk2) activation Carcinogenesis, May 1, 2004; 25(5): 669 - 680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. E.J Teunissen and M. F.A Bierhuizen Transcriptional control of myocardial connexins Cardiovasc Res, May 1, 2004; 62(2): 246 - 255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Spinella, L. Rosano, V. Di Castro, M. R. Nicotra, P. G. Natali, and A. Bagnato Endothelin-1 Decreases Gap Junctional Intercellular Communication by Inducing Phosphorylation of Connexin 43 in Human Ovarian Carcinoma Cells J. Biol. Chem., October 17, 2003; 278(42): 41294 - 41301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. SAEZ, V. M. BERTHOUD, M. C. BRANES, A. D. MARTINEZ, and E. C. BEYER Plasma Membrane Channels Formed by Connexins: Their Regulation and Functions Physiol Rev, October 1, 2003; 83(4): 1359 - 1400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Easwaran, S. H. Lee, L. Inge, L. Guo, C. Goldbeck, E. Garrett, M. Wiesmann, P. D. Garcia, J. H. Fuller, V. Chan, et al. {beta}-Catenin Regulates Vascular Endothelial Growth Factor Expression in Colon Cancer Cancer Res., June 15, 2003; 63(12): 3145 - 3153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A.G. van der Heyden and L. H.K. Defize Twenty one years of P19 cells: what an embryonal carcinoma cell line taught us about cardiomyocyte differentiation Cardiovasc Res, May 1, 2003; 58(2): 292 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A.G. van der Heyden, M. J.A. van Kempen, Y. Tsuji, M. B. Rook, H. J. Jongsma, and T. Opthof P19 embryonal carcinoma cells: a suitable model system for cardiac electrophysiological differentiation at the molecular and functional level Cardiovasc Res, May 1, 2003; 58(2): 410 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Husoy, V. Cruciani, H. K. Knutsen, S.-O. Mikalsen, H. B. Olstorn, and J. Alexander Cells heterozygous for the ApcMin mutation have decreased gap junctional intercellular communication and connexin43 level, and reduced microtubule polymerization Carcinogenesis, April 1, 2003; 24(4): 643 - 650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Carystinos, M. Kandouz, M. A. Alaoui-Jamali, and G. Batist Unexpected Induction of the Human Connexin 43 Promoter by the Ras Signaling Pathway Is Mediated by a Novel Putative Promoter Sequence Mol. Pharmacol., April 1, 2003; 63(4): 821 - 831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Schwarz, I. Wanke, U. Wulbrand, O. Moennikes, and A. Buchmann Role of Connexin32 and {beta}-Catenin in Tumor Promotion in Mouse Liver Toxicol Pathol, January 1, 2003; 31(1): 99 - 102. [Abstract] [PDF]
|
|
|
|
|
|
W. Holnthoner, M. Pillinger, M. Groger, K. Wolff, A. W. Ashton, C. Albanese, P. Neumeister, R. G. Pestell, and P. Petzelbauer Fibroblast Growth Factor-2 Induces Lef/Tcf-dependent Transcription in Human Endothelial Cells J. Biol. Chem., November 22, 2002; 277(48): 45847 - 45853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Koval Sharing signals: connecting lung epithelial cells with gap junction channels Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L875 - L893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hadjiargyrou, F. Lombardo, S. Zhao, W. Ahrens, J. Joo, H. Ahn, M. Jurman, D. W. White, and C. T. Rubin Transcriptional Profiling of Bone Regeneration. INSIGHT INTO THE MOLECULAR COMPLEXITY OF WOUND REPAIR J. Biol. Chem., August 9, 2002; 277(33): 30177 - 30182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhai, R. Wu, D. R. Schwartz, D. Darrah, H. Reed, F. T. Kolligs, M. T. Nieman, E. R. Fearon, and K. R. Cho Role of {beta}-Catenin/T-Cell Factor-Regulated Genes in Ovarian Endometrioid Adenocarcinomas Am. J. Pathol., April 1, 2002; 160(4): 1229 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Ryu, S.-J. Kim, S.-H. Kim, C.-D. Oh, S.-G. Hwang, C.-H. Chun, S.-H. Oh, J.-K. Seong, T.-L. Huh, and J.-S. Chun Regulation of the chondrocyte phenotype by {beta}-catenin Development, January 12, 2002; 129(23): 5541 - 5550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Bauer, C. Lehmann, B. Fuss, F. Eckardt, and M. Hoch The Drosophila gap junction channel gene innexin 2 controls foregut development in response to Wingless signalling J. Cell Sci., January 5, 2002; 115(9): 1859 - 1867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wu, Y. Zhai, E. R. Fearon, and K. R. Cho Diverse Mechanisms of {beta}-Catenin Deregulation in Ovarian Endometrioid Adenocarcinomas Cancer Res., November 1, 2001; 61(22): 8247 - 8255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Tao, D. Pennica, L. Xu, R. F. Kalejta, and A. J. Levine Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1 Genes & Dev., July 15, 2001; 15(14): 1796 - 1807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Xu, W.E.I. Li, G.Y. Huang, R. Meyer, T. Chen, Y. Luo, M.P. Thomas, G.L. Radice, and C.W. Lo Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions J. Cell Biol., July 9, 2001; 154(1): 217 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. S. Fearnhead, M. P. Britton, and W. F. Bodmer The ABC of APC Hum. Mol. Genet., April 1, 2001; 10(7): 721 - 733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Takayasu, H. Horie, E. Hiyama, T. Matsunaga, Y. Hayashi, Y. Watanabe, S. Suita, M. Kaneko, F. Sasaki, K. Hashizume, et al. Frequent Deletions and Mutations of the {beta}-Catenin Gene Are Associated with Overexpression of Cyclin D1 and Fibronectin and Poorly Differentiated Histology in Childhood Hepatoblastoma Clin. Cancer Res., April 1, 2001; 7(4): 901 - 908. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. M. Monick, A. B. Carter, P. K. Robeff, D. M. Flaherty, M. W. Peterson, and G. W. Hunninghake Lipopolysaccharide Activates Akt in Human Alveolar Macrophages Resulting in Nuclear Accumulation and Transcriptional Activity of {{beta}}-Catenin J. Immunol., April 1, 2001; 166(7): 4713 - 4720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. T. Ziemer, D. Pennica, and A. J. Levine Identification of a Mouse Homolog of the Human BTEB2 Transcription Factor as a {beta}-Catenin-Independent Wnt-1-Responsive Gene Mol. Cell. Biol., January 15, 2001; 21(2): 562 - 574. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Lo Muzio A Possible Role for the Wnt~1 Pathway in Oral Carcinogenesis Critical Reviews in Oral Biology & Medicine, January 1, 2001; 12(2): 152 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kim, K. M. Pang, M. Evans, and E. D. Hay Overexpression of beta -Catenin Induces Apoptosis Independent of Its Transactivation Function with LEF-1 or the Involvement of Major G1 Cell Cycle Regulators Mol. Biol. Cell, October 1, 2000; 11(10): 3509 - 3523. [Abstract] [Full Text]
|
|
|
|
|
|
T. Toyofuku, Z. Hong, T. Kuzuya, M. Tada, and M. Hori Wnt/frizzled-2 Signaling Induces Aggregation and Adhesion among Cardiac Myocytes by Increased Cadherin-{beta}-Catenin Complex J. Cell Biol., July 11, 2000; 150(1): 225 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Paulson, P. Lampe, R. Meyer, E TenBroek, M. Atkinson, T. Walseth, and R. Johnson Cyclic AMP and LDL trigger a rapid enhancement in gap junction assembly through a stimulation of connexin trafficking J. Cell Sci., January 9, 2000; 113(17): 3037 - 3049. [Abstract] [PDF]
|
|
|
|
 |
|
 E. Pötter, C. Bergwitz, and G. Brabant The Cadherin-Catenin System: Implications for Growth and Differentiation of Endocrine Tissues Endocr. Rev., April 1, 1999; 20(2): 207 - 239. [Abstract] [Full Text]
|
|
|
|
|
|
M. Haertel-Wiesmann, Y. Liang, W. J. Fantl, and L. T. Williams Regulation of Cyclooxygenase-2 and Periostin by Wnt-3 in Mouse Mammary Epithelial Cells J. Biol. Chem., October 6, 2000; 275(41): 32046 - 32051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Meijer, W. J. A. G. Dictus, E. D. Keuning, and A. A. M. Thomas Translational Control of the Xenopus laevis Connexin-41 5'-Untranslated Region by Three Upstream Open Reading Frames J. Biol. Chem., September 29, 2000; 275(40): 30787 - 30793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Song, D. J. Sussman, and D. C. Seldin Endogenous Protein Kinase CK2 Participates in Wnt Signaling in Mammary Epithelial Cells J. Biol. Chem., July 28, 2000; 275(31): 23790 - 23797. [Abstract] [Full Text] [PDF]
|
|
