|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 13 1767-1778, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
CL Ho, JL Martys, A Mikhailov, GG Gundersen and RK Liem
Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
In order to study the dynamic behavior of intermediate filament networks in living cells, we have prepared constructs fusing green fluorescent protein to intermediate filament proteins. Vimentin fused to green fluorescent protein labeled the endogenous intermediate filament network. We generated stable SW13 and NIH3T3 cell lines that express an enhanced green fluorescent protein fused to the N-terminus of full-length vimentin. We were able to observe the dynamic behavior of the intermediate filament network in these cells for periods as long as 4 hours (images acquired every 2 minutes). In both cell lines, the vimentin network constantly moves in a wavy manner. In the NIH3T3 cells, we observed extension of individual vimentin filaments at the edge of the cell. This movement is dependent on microtubules, since the addition of nocodazole stopped the extension of the intermediate filaments. Injection of anti-IFA causes the redistribution or 'collapse' of intermediate filaments. We injected anti-IFA antibodies into NIH3T3 cells stably expressing green fluorescent protein fused to vimentin and found that individual intermediate filaments move slowly towards the perinuclear area without obvious disassembly. These results demonstrate that individual intermediate filaments are translocated during the collapse, rather than undergoing disassembly-induced redistribution. Injections of tubulin antibodies disrupt the interactions between intermediate filaments and stable microtubules and cause the collapse of the vimentin network showing that these interactions play an important role in keeping the intermediate filament network extended. The nocodazole inhibition of intermediate filament extension and the anti-IFA microinjection experiments are consistent with a model in which intermediate filaments exhibit an extended distribution when tethered to microtubules, but are translocated to the perinuclear area when these connections are severed.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  E. W. Flitney, E. R. Kuczmarski, S. A. Adam, and R. D. Goldman Insights into the mechanical properties of epithelial cells: the effects of shear stress on the assembly and remodeling of keratin intermediate filaments FASEB J, July 1, 2009; 23(7): 2110 - 2119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Charbon, M. T. Cabeen, and C. Jacobs-Wagner Bacterial intermediate filaments: in vivo assembly, organization, and dynamics of crescentin Genes & Dev., May 1, 2009; 23(9): 1131 - 1144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Cleveland, K. Yamanaka, and P. Bomont Gigaxonin controls vimentin organization through a tubulin chaperone-independent pathway Hum. Mol. Genet., April 15, 2009; 18(8): 1384 - 1394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Bartolini, J. B. Moseley, J. Schmoranzer, L. Cassimeris, B. L. Goode, and G. G. Gundersen The formin mDia2 stabilizes microtubules independently of its actin nucleation activity J. Cell Biol., October 14, 2008; 181(3): 523 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-D. Perng, S.-F. Wen, T. Gibbon, J. Middeldorp, J. Sluijs, E. M. Hol, and R. A. Quinlan Glial Fibrillary Acidic Protein Filaments Can Tolerate the Incorporation of Assembly-compromised GFAP-{delta}, but with Consequences for Filament Organization and {alpha}B-Crystallin Association Mol. Biol. Cell, October 1, 2008; 19(10): 4521 - 4533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kueper, T. Grune, S. Prahl, H. Lenz, V. Welge, T. Biernoth, Y. Vogt, G.-M. Muhr, A. Gaemlich, T. Jung, et al. Vimentin Is the Specific Target in Skin Glycation: STRUCTURAL PREREQUISITES, FUNCTIONAL CONSEQUENCES, AND ROLE IN SKIN AGING J. Biol. Chem., August 10, 2007; 282(32): 23427 - 23436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yang, J. Gao, M. Adamian, X.-H. Wen, B. Pawlyk, L. Zhang, M. J. Sanderson, J. Zuo, C. L. Makino, and T. Li The Ciliary Rootlet Maintains Long-Term Stability of Sensory Cilia Mol. Cell. Biol., May 15, 2005; 25(10): 4129 - 4137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Perlson, K. F. Medzihradszky, Z. Darula, D. W. Munno, N. I. Syed, A. L. Burlingame, and M. Fainzilber Differential Proteomics Reveals Multiple Components in Retrogradely Transported Axoplasm After Nerve Injury Mol. Cell. Proteomics, May 1, 2004; 3(5): 510 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Windoffer, S. Woll, P. Strnad, and R. E. Leube Identification of Novel Principles of Keratin Filament Network Turnover in Living Cells Mol. Biol. Cell, May 1, 2004; 15(5): 2436 - 2448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Werner, R. Windoffer, P. Strnad, C. Grund, R. E. Leube, and T. M. Magin Epidermolysis Bullosa Simplex-Type Mutations Alter the Dynamics of the Keratin Cytoskeleton and Reveal a Contribution of Actin to the Transport of Keratin Subunits Mol. Biol. Cell, March 1, 2004; 15(3): 990 - 1002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. T. Helfand, L. Chang, and R. D. Goldman Intermediate filaments are dynamic and motile elements of cellular architecture J. Cell Sci., January 15, 2004; 117(2): 133 - 141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. T. Helfand, P. Loomis, M. Yoon, and R. D. Goldman Rapid transport of neural intermediate filament protein J. Cell Sci., June 1, 2003; 116(11): 2345 - 2359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liovic, M. M. Mogensen, A. R. Prescott, and E. B. Lane Observation of keratin particles showing fast bidirectional movement colocalized with microtubules J. Cell Sci., April 15, 2003; 116(8): 1417 - 1427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Goto, Y. Yasui, A. Kawajiri, E. A. Nigg, Y. Terada, M. Tatsuka, K.-i. Nagata, and M. Inagaki Aurora-B Regulates the Cleavage Furrow-specific Vimentin Phosphorylation in the Cytokinetic Process J. Biol. Chem., February 28, 2003; 278(10): 8526 - 8530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ashok and W. J. Atwood Contrasting Roles of Endosomal pH and the Cytoskeleton in Infection of Human Glial Cells by JC Virus and Simian Virus 40 J. Virol., December 20, 2002; 77(2): 1347 - 1356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Delhaas, S. van Engeland, J. Broers, C. Bouten, N. Kuijpers, F. Ramaekers, and L. H. E. H. Snoeckx Quantification of cytoskeletal deformation in living cells based on hierarchical feature vector matching Am J Physiol Cell Physiol, August 1, 2002; 283(2): C639 - C645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Xu, T. Sikaneta, B. M. Sullivan, Q. Zhang, M. Andreucci, T. Stehle, I. Drummond, and M. A. Arnaout Polycystin-1 Interacts with Intermediate Filaments J. Biol. Chem., November 30, 2001; 276(49): 46544 - 46552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Yoon, M. Yoon, R. D. Moir, S. Khuon, F. W. Flitney, and R. D. Goldman Insights into the Dynamic Properties of Keratin Intermediate Filaments in Living Epithelial Cells J. Cell Biol., April 30, 2001; 153(3): 503 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Roy, P. Coffee, G. Smith, R. K. H. Liem, S. T. Brady, and M. M. Black Neurofilaments Are Transported Rapidly But Intermittently in Axons: Implications for Slow Axonal Transport J. Neurosci., September 15, 2000; 20(18): 6849 - 6861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Chou and R. D. Goldman Intermediate Filaments on the Move J. Cell Biol., August 7, 2000; 150(3): 101 - 106. [Full Text] [PDF]
|
|
|
|
|
|
S. Ackerley, A. J. Grierson, J. Brownlees, P. Thornhill, B. H. Anderton, P. N. Leigh, C. E. Shaw, and C. C.J. Miller Glutamate Slows Axonal Transport of Neurofilaments in Transfected Neurons J. Cell Biol., July 10, 2000; 150(1): 165 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Morris, K Evason, C Wiand, T. L'Ecuyer, and A. Fulton Misdirected vimentin messenger RNA alters cell morphology and motility J. Cell Sci., January 7, 2000; 113(13): 2433 - 2443. [Abstract] [PDF]
|
|
|
|
|
|
J. L. Martys, C.-L. Ho, R. K. H. Liem, and G. G. Gundersen Intermediate Filaments in Motion: Observations of Intermediate Filaments in Cells Using Green Fluorescent Protein-Vimentin Mol. Biol. Cell, May 1, 1999; 10(5): 1289 - 1295. [Full Text]
|
|
|
|
|
|
G. Kreitzer, G. Liao, and G. G. Gundersen Detyrosination of Tubulin Regulates the Interaction of Intermediate Filaments with Microtubules In Vivo via a Kinesin-dependent Mechanism Mol. Biol. Cell, April 1, 1999; 10(4): 1105 - 1118. [Abstract] [Full Text]
|
|
|
|
|
|
R Windoffer and R. Leube Detection of cytokeratin dynamics by time-lapse fluorescence microscopy in living cells J. Cell Sci., January 12, 1999; 112(24): 4521 - 4534. [Abstract] [PDF]
|
|
|
|
|
|
J. Yabe, A Pimenta, and T. Shea Kinesin-mediated transport of neurofilament protein oligomers in growing axons J. Cell Sci., January 11, 1999; 112(21): 3799 - 3814. [Abstract] [PDF]
|
|
|
|
|
|
W.-C. Sin, X.-Q. Chen, T. Leung, and L. Lim RhoA-Binding Kinase alpha Translocation Is Facilitated by the Collapse of the Vimentin Intermediate Filament Network Mol. Cell. Biol., November 1, 1998; 18(11): 6325 - 6339. [Abstract] [Full Text]
|
|
|
|
|
|
M. Yoon, R. D. Moir, V. Prahlad, and R. D. Goldman Motile Properties of Vimentin Intermediate Filament Networks in Living Cells J. Cell Biol., October 5, 1998; 143(1): 147 - 157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Meriane, S. Mary, F. Comunale, E. Vignal, P. Fort, and C. Gauthier-Rouviere Cdc42Hs and Rac1 GTPases Induce the Collapse of the Vimentin Intermediate Filament Network J. Biol. Chem., October 13, 2000; 275(42): 33046 - 33052. [Abstract] [Full Text] [PDF]
|
|
