|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 14 1973-1980, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
S Valgeirsdottir, L Claesson-Welsh, E Bongcam-Rudloff, U Hellman, B Westermark and CH Heldin
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
In this study we demonstrate that stimulation with platelet-derived growth factor (PDGF) leads to a marked reorganization of the vimentin filaments in porcine aortic endothelial (PAE) cells ectopically expressing the PDGF beta-receptor. Within 20 minutes after stimulation, the well-spread fine fibrillar vimentin was reorganized as the filaments aggregated into a dense coil around the nucleus. The solubility of vimentin upon Nonidet-P40-extraction of cells decreased considerably after PDGF stimulation, indicating that PDGF caused a redistribution of vimentin to a less soluble compartment. In addition, an increased tyrosine phosphorylation of vimentin was observed. The redistribution of vimentin was not a direct consequence of its tyrosine phosphorylation, since treatment of cells with an inhibitor for the cytoplasmic tyrosine kinase Src, attenuated phosphorylation but not redistribution of vimentin. These changes in the distribution of vimentin occurred in conjunction with reorganization of actin filaments. In PAE cells expressing a Y740/751F mutant receptor that is unable to bind and activate phosphatidylinositol 3'-kinase (PI3-kinase), the distribution of vimentin was virtually unaffected by PDGF stimulation. Thus, PI3-kinase is important for vimentin reorganization, in addition to its previously demonstrated role in actin reorganization. The small GTPase Rac has previously been shown to be involved downstream of PI3-kinase in the reorganization of actin filaments. In PAE cells overexpressing dominant negative Rac1 (N17Rac1), no change in the fine fibrillar vimentin network was seen after PDGF-BB stimulation, whereas in PAE cells overexpressing constitutively active Rac1 (V12Rac1), there was a dramatic change in vimentin filament organization independent of PDGF stimulation. These data indicate that PDGF causes a reorganization of microfilaments as well as intermediate filaments in its target cells and suggest an important role for Rac downstream of PI3-kinase in the PDGF stimulated reorganization of both actin and vimentin filaments.
This article has been cited by other articles:
|
|
 |
|
  K. W. Kang, S. J. Lee, J. W. Park, and S. G. Kim Phosphatidylinositol 3-Kinase Regulates Nuclear Translocation of NF-E2-Related Factor 2 through Actin Rearrangement in Response to Oxidative Stress Mol. Pharmacol., November 1, 2002; 62(5): 1001 - 1010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Kim, E. J. Song, and K.-J. Lee Proteomic Analysis of Protein Phosphorylations in Heat Shock Response and Thermotolerance J. Biol. Chem., June 21, 2002; 277(26): 23193 - 23207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Ridley Rho GTPases and cell migration J. Cell Sci., January 8, 2001; 114(15): 2713 - 2722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sanlioglu, P. K. Benson, J. Yang, E. M. Atkinson, T. Reynolds, and J. F. Engelhardt Endocytosis and Nuclear Trafficking of Adeno-Associated Virus Type 2 Are Controlled by Rac1 and Phosphatidylinositol-3 Kinase Activation J. Virol., October 1, 2000; 74(19): 9184 - 9196. [Abstract] [Full Text]
|
|
|
|
|
|
C Gilles, M Polette, J. Zahm, J. Tournier, L Volders, J. Foidart, and P Birembaut Vimentin contributes to human mammary epithelial cell migration J. Cell Sci., January 12, 1999; 112(24): 4615 - 4625. [Abstract] [PDF]
|
|
|
|
|
|
L Feng, X Zhou, J Liao, and M. Omary Pervanadate-mediated tyrosine phosphorylation of keratins 8 and 19 via a p38 mitogen-activated protein kinase-dependent pathway J. Cell Sci., January 7, 1999; 112(13): 2081 - 2090. [Abstract] [PDF]
|
|
|
|
|
|
M. Meriane, S. Mary, F. Comunale, E. Vignal, P. Fort, and C. Gauthier-Rouviere Cdc42Hs and Rac1 GTPases Induce the Collapse of the Vimentin Intermediate Filament Network J. Biol. Chem., October 13, 2000; 275(42): 33046 - 33052. [Abstract] [Full Text] [PDF]
|
|
