|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 14 2017-2027, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
KA Swan, AF Severson, JC Carter, PR Martin, H Schnabel, R Schnabel and B Bowerman
Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
A maternally expressed Caenorhabditis elegans gene called cyk-1 is required for polar body extrusion during meiosis and for a late step in cytokinesis during embryonic mitosis. Other microfilament- and microtubule-dependent processes appear normal in cyk-1 mutant embryos, indicating that cyk-1 regulates a specific subset of cytoskeletal functions. Because cytokinesis initiates normally and cleavage furrows ingress extensively in cyk-1 mutant embryos, we propose that the wild-type cyk-1 gene is required for a late step in cytokinesis. Cleavage furrows regress after completion of mitosis in cyk-1 mutants, leaving multiple nuclei in a single cell. Positional cloning and sequence analysis of the cyk-1 gene reveal that it encodes an FH protein, a newly defined family of proteins that appear to interact with the cytoskeleton during cytokinesis and in the regulation of cell polarity. Consistent with cyk-1 function being required for a late step in embryonic cytokinesis, we show that the CYK-1 protein co-localizes with actin microfilaments as a ring at the leading edge of the cleavage furrow, but only after extensive furrow ingression. We discuss our findings in the context of other studies suggesting that FH genes in yeast and insects function early in cytokinesis to assemble a cleavage furrow.
This article has been cited by other articles:
|
|
 |
|
  E. M. Neidt, C. T. Skau, and D. R. Kovar The Cytokinesis Formins from the Nematode Worm and Fission Yeast Differentially Mediate Actin Filament Assembly J. Biol. Chem., August 29, 2008; 283(35): 23872 - 23883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. F. Homem and M. Peifer Diaphanous regulates myosin and adherens junctions to control cell contractility and protrusive behavior during morphogenesis Development, March 15, 2008; 135(6): 1005 - 1018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Thullberg, A. Gad, S. Le Guyader, and S. Stromblad Oncogenic H-Ras V12 promotes anchorage-independent cytokinesis in human fibroblasts PNAS, December 18, 2007; 104(51): 20338 - 20343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhang and D. N. Robinson Balance of actively generated contractile and resistive forces controls cytokinesis dynamics PNAS, May 17, 2005; 102(20): 7186 - 7191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. D'Avino, M. S. Savoian, and D. M. Glover Cleavage furrow formation and ingression during animal cytokinesis: a microtubule legacy J. Cell Sci., April 15, 2005; 118(8): 1549 - 1558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Glotzer The Molecular Requirements for Cytokinesis Science, March 18, 2005; 307(5716): 1735 - 1739. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. D'Avino, M. S. Savoian, and D. M. Glover Mutations in sticky lead to defective organization of the contractile ring during cytokinesis and are enhanced by Rho and suppressed by Rac J. Cell Biol., July 5, 2004; 166(1): 61 - 71. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Bito Dynamic Control of Neuronal Morphogenesis by Rho Signaling J. Biochem., September 1, 2003; 134(3): 315 - 319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Evangelista, S. Zigmond, and C. Boone Formins: signaling effectors for assembly and polarization of actin filaments J. Cell Sci., July 1, 2003; 116(13): 2603 - 2611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ono, M. Parast, C. Alberico, G. M. Benian, and S. Ono Specific requirement for two ADF/cofilin isoforms in distinct actin-dependent processes in Caenorhabditis elegans J. Cell Sci., May 15, 2003; 116(10): 2073 - 2085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kitayama and T. Q. P. Uyeda ForC, a novel type of formin family protein lacking an FH1 domain, is involved in multicellular development in Dictyostelium discoideum J. Cell Sci., February 15, 2003; 116(4): 711 - 723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Herrmann, T. Dittmar, and K. S. Erdmann The Protein Tyrosine Phosphatase PTP-BL Associates with the Midbody and Is Involved in the Regulation of Cytokinesis Mol. Biol. Cell, January 1, 2003; 14(1): 230 - 240. [Abstract] [Full Text]
|
|
|
|
|
|
U. Gruneberg, M. Glotzer, A. Gartner, and E. A. Nigg The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo J. Cell Biol., September 3, 2002; 158(5): 901 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Litvak, D. Tian, S. Carmon, and S. Lev Nir2, a Human Homolog of Drosophila melanogaster Retinal Degeneration B Protein, Is Essential for Cytokinesis Mol. Cell. Biol., July 15, 2002; 22(14): 5064 - 5075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Somma, B. Fasulo, G. Cenci, E. Cundari, and M. Gatti Molecular Dissection of Cytokinesis by RNA Interference in Drosophila Cultured Cells Mol. Biol. Cell, July 1, 2002; 13(7): 2448 - 2460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Guertin, S. Trautmann, and D. McCollum Cytokinesis in Eukaryotes Microbiol. Mol. Biol. Rev., June 1, 2002; 66(2): 155 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakano, J. Imai, R. Arai, A. Toh-e, Y. Matsui, and I. Mabuchi The small GTPase Rho3 and the diaphanous/formin For3 function in polarized cell growth in fission yeast J. Cell Sci., January 12, 2002; 115(23): 4629 - 4639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Piekny and P. E. Mains Rho-binding kinase (LET-502) and myosin phosphatase (MEL-11) regulate cytokinesis in the early Caenorhabditis elegans embryo J. Cell Sci., January 6, 2002; 115(11): 2271 - 2282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fares and I. Greenwald Genetic Analysis of Endocytosis in Caenorhabditis elegans: Coelomocyte Uptake Defective Mutants Genetics, September 1, 2001; 159(1): 133 - 145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Golden, P. L. Sadler, M. R. Wallenfang, J. M. Schumacher, D. R. Hamill, G. Bates, B. Bowerman, G. Seydoux, and D. C. Shakes Metaphase to Anaphase (mat) Transition-defective Mutants in Caenorhabditis elegans J. Cell Biol., December 27, 2000; 151(7): 1469 - 1482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Jantsch-Plunger, P. Gonczy, A. Romano, H. Schnabel, D. Hamill, R. Schnabel, A. A. Hyman, and M. Glotzer CYK-4: A Rho Family GTPase Activating Protein (GAP) Required for Central Spindle Formation and Cytokinesis J. Cell Biol., June 26, 2000; 149(7): 1391 - 1404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G Manandhar, R. Moreno, C Simerly, K Toshimori, and G Schatten Contractile apparatus of the normal and abortive cytokinetic cells during mouse male meiosis J. Cell Sci., January 12, 2000; 113(23): 4275 - 4286. [Abstract] [PDF]
|
|
|
|
|
|
M. A. Lorson, H. R. Horvitz, and S. van den Heuvel LIN-5 Is a Novel Component of the Spindle Apparatus Required for Chromosome Segregation and Cleavage Plane Specification in Caenorhabditis elegans J. Cell Biol., January 10, 2000; 148(1): 73 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Brill, G. Hime, M Scharer-Schuksz, and M. Fuller A phospholipid kinase regulates actin organization and intercellular bridge formation during germline cytokinesis Development, January 9, 2000; 127(17): 3855 - 3864. [Abstract] [PDF]
|
|
|
|
|
|
F Motegi, K Nakano, and I Mabuchi Molecular mechanism of myosin-II assembly at the division site in Schizosaccharomyces pombe J. Cell Sci., January 5, 2000; 113(10): 1813 - 1825. [Abstract] [PDF]
|
|
|
|
|
|
C. Magie, M. Meyer, M. Gorsuch, and S. Parkhurst Mutations in the Rho1 small GTPase disrupt morphogenesis and segmentation during early Drosophila development Development, January 12, 1999; 126(23): 5353 - 5364. [Abstract] [PDF]
|
|
|
|
|
|
J. M. Schumacher, A. Golden, and P. J. Donovan AIR-2: An Aurora/Ipl1-related Protein Kinase Associated with Chromosomes and Midbody Microtubules Is Required for Polar Body Extrusion and Cytokinesis in Caenorhabditis elegans Embryos J. Cell Biol., December 14, 1998; 143(6): 1635 - 1646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Demeter and S. Sazer imp2, a New Component of the Actin Ring in the Fission Yeast Schizosaccharomyces pombe J. Cell Biol., October 19, 1998; 143(2): 415 - 427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Sharpless and S. D. Harris Functional Characterization and Localization of the Aspergillus nidulans Formin SEPA Mol. Biol. Cell, February 1, 2002; 13(2): 469 - 479. [Abstract] [Full Text] [PDF]
|
|
