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Journal of Cell Science, Vol 111, Issue 17 2507-2518, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
C De Nadai, P Huitorel, S Chiri and B Ciapa
Groupe de Recherche sur l'Interaction Gametique (GRIG), CJF 9504 INSERM, Faculte de Medecine, Avenue de Valombrose, France. ciapa@ccrv.obs-vlfr.fr
We have reported earlier that the polyphosphoinositide messenger system may control mitosis in sea urchin eggs. Besides phospholipase C activation and its second messengers, phosphatidylinositol (PI) 3-kinase has been proposed to affect a wide variety of cellular processes in other cellular systems. Therefore, we have investigated whether PI 3-kinase could play a role in regulating the sea urchin early embryonic development. Our data presented here suggest that PI 3-kinase is present in sea urchin eggs. We found that wortmannin, an inhibitor of PI 3-kinase, led to arrest of the cell cycle. Chromosome condensation, nuclear envelope breakdown, microtubular aster polymerization, protein and DNA synthesis were not affected when fertilization was performed in the presence of the drug. However, maturation-promoting factor (MPF) activation was inhibited and centrosome duplication was perturbed preventing the formation of a bipolar mitotic spindle in wortmannin treated eggs. We discuss how PI 3-kinase might be involved in the cascade of events leading to the first mitotic divisions of the fertilized sea urchin egg.
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W. L. Zhang, P. Huitorel, A.-M. Geneviere, S. Chiri, and B. Ciapa Inactivation of MAPK in mature oocytes triggers progression into mitosis via a Ca2+-dependent pathway but without completion of S phase J. Cell Sci., September 1, 2006; 119(17): 3491 - 3501. [Abstract] [Full Text] [PDF]
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P. Salaun, S. Boulben, O. Mulner-Lorillon, R. Belle, N. Sonenberg, J. Morales, and P. Cormier Embryonic-stage-dependent changes in the level of eIF4E-binding proteins during early development of sea urchin embryos J. Cell Sci., April 1, 2005; 118(7): 1385 - 1394. [Abstract] [Full Text] [PDF]
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 E. C. Roberts, P. S. Shapiro, T. S. Nahreini, G. Pages, J. Pouyssegur, and N. G. Ahn Distinct Cell Cycle Timing Requirements for Extracellular Signal-Regulated Kinase and Phosphoinositide 3-Kinase Signaling Pathways in Somatic Cell Mitosis Mol. Cell. Biol., October 15, 2002; 22(20): 7226 - 7241. [Abstract] [Full Text] [PDF]
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 J Shearer, C De Nadai, F Emily-Fenouil, C Gache, M Whitaker, and B Ciapa Role of phospholipase Cgamma at fertilization and during mitosis in sea urchin eggs and embryos Development, January 5, 1999; 126(10): 2273 - 2284. [Abstract] [PDF]
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S Chiri, C De Nadai, and B Ciapa Evidence for MAP kinase activation during mitotic division J. Cell Sci., January 9, 1998; 111(17): 2519 - 2527. [Abstract] [PDF]
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