Journal of Cell Science, Vol 111, Issue 17 2519-2527, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

Evidence for MAP kinase activation during mitotic division

S Chiri, C De Nadai and B Ciapa
Groupe de Recherche Sur l'Interaction Gametique (GRIG), CJF 9504 INSERM, Faculte de Medecine, Avenue de Valombrose, Cedex 02 France.

MAP kinases have been implicated in the control of a broad spectrum of cellular events in many types of cells. In somatic cells, MAP kinase activation seems to be triggered after exit from a quiescent state (in G0 or G2) only and then inactivated by entry into a proliferative state. In oocytes of various species, a one-time activation of MAP kinase that is apparently not repeated during the succeeding mitotic cycles occurs after meiotic activation. However, several reports suggest that a myelin basic protein (MBP) kinase activity, unrelated to that of maturation promoting factor, can sometimes be detected during mitotic divisions in various types of cells and oocytes. We have reinvestigated this problem in order to determine the origin and the role of MBP kinase that is stimulated at time of mitosis in the fertilized eggs of the sea urchin Paracentrotus lividus. We used anti-ERK1 antibodies or substrates specific for different MAP kinases, and performed in-gel phosphorylation experiments. Our results suggest that an ERK1-like protein was responsible for part of the MBP kinase activity that is stimulated during the first mitotic divisions. Furthermore, we observed that wortmannin, an inhibitor of PI 3-kinase that arrests the fertilized sea urchin eggs at the prometaphase stage, inhibited the inactivation of MAP kinase normally observed when the eggs divide, suggesting a role for PI 3-kinase in the deactivation process of MAP kinase. We also discuss how the activities of MPF and MAP kinase may be interconnected to regulate the first mitotic divisions of the early sea urchin embryo.

This article has been cited by other articles:

				W. L. Zhang, P. Huitorel, A.-M. Geneviere, S. Chiri, and B. Ciapa Inactivation of MAPK in mature oocytes triggers progression into mitosis via a Ca2+-dependent pathway but without completion of S phase J. Cell Sci., September 1, 2006; 119(17): 3491 - 3501. [Abstract] [Full Text] [PDF]

				R. Philipova, M. G. Larman, C. P. Leckie, P. K. Harrison, L. Groigno, and M. Whitaker Inhibiting MAP Kinase Activity Prevents Calcium Transients and Mitosis Entry in Early Sea Urchin Embryos J. Biol. Chem., July 1, 2005; 280(26): 24957 - 24967. [Abstract] [Full Text] [PDF]

				R. Philipova, J. Kisielewska, P. Lu, M. Larman, J.-Y. Huang, and M. Whitaker ERK1 activation is required for S-phase onset and cell cycle progression after fertilization in sea urchin embryos Development, February 1, 2005; 132(3): 579 - 589. [Abstract] [Full Text] [PDF]

				M. A. Morgan, O. Dolp, and C. W. M. Reuter Cell-cycle-dependent activation of mitogen-activated protein kinase kinase (MEK-1/2) in myeloid leukemia cell lines and induction of growth inhibition and apoptosis by inhibitors of RAS signaling Blood, March 15, 2001; 97(6): 1823 - 1834. [Abstract] [Full Text] [PDF]

				M Levasseur and A McDougall Sperm-induced calcium oscillations at fertilisation in ascidians are controlled by cyclin B1-dependent kinase activity Development, January 2, 2000; 127(3): 631 - 641. [Abstract] [PDF]

				L. Bögre, O. Calderini, P. Binarova, M. Mattauch, S. Till, S. Kiegerl, C. Jonak, C. Pollaschek, P. Barker, N. S. Huskisson, et al. A MAP Kinase Is Activated Late in Plant Mitosis and Becomes Localized to the Plane of Cell Division PLANT CELL, January 1, 1999; 11(1): 101 - 114. [Abstract] [Full Text]

				T. M. Guadagno and J. E. Ferrell Jr. Requirement for MAPK Activation for Normal Mitotic Progression in Xenopus Egg Extracts Science, November 13, 1998; 282(5392): 1312 - 1315. [Abstract] [Full Text]

				R Philipova and M Whitaker MAP kinase activity increases during mitosis in early sea urchin embryos J. Cell Sci., January 9, 1998; 111(17): 2497 - 2505. [Abstract] [PDF]

				C De Nadai, P Huitorel, S Chiri, and B Ciapa Effect of wortmannin, an inhibitor of phosphatidylinositol 3-kinase, on the first mitotic divisions of the fertilized sea urchin egg J. Cell Sci., January 9, 1998; 111(17): 2507 - 2518. [Abstract] [PDF]