|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 17 2681-2688, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
K Riento, T Galli, S Jansson, C Ehnholm, E Lehtonen and VM Olkkonen
Department of Biochemistry, National Public Health Institute, Mannerheimintie 166, FIN-00300, Helsinki, Finland.
The docking/fusion of transport vesicles mediated by the soluble NSF attachment protein receptors (SNAREs) is thought to be regulated by Sec1-related proteins. Munc-18-2, a member of this family, is predominantly expressed in the epithelial cells of several tissues. We demonstrate here that Munc-18-2 colocalizes with syntaxin 3 at the apical plasma membrane of intestinal epithelium and Caco-2 cells. The presence of a physical complex of the two proteins is verified by 2-way coimmunoprecipitation. The quantity of the complex is reduced by treatment of Caco-2 cells with the alkylating agent N-ethylmaleimide which also has an inhibitory effect on the ability of Munc-18-2 to associate with syntaxin 3 in vitro. The amount of Munc-18-2 in the complex increases upon treatment of the cells with the protein kinase C activator phorbol myristate acetate, indicating a functional connection between the complex and cell signalling. Increasing the amount of Munc-18-2 bound to syntaxin 3 by overexpression results in a marked decrease in the SNARE proteins SNAP-23 and cellubrevin bound to the syntaxin. These results define a novel functional complex of Munc-18-2 and syntaxin 3 involved in the regulation of apical membrane transport.
This article has been cited by other articles:
|
|
 |
|
  G. Procino, C. Barbieri, G. Tamma, L. De Benedictis, J. E. Pessin, M. Svelto, and G. Valenti AQP2 exocytosis in the renal collecting duct - involvement of SNARE isoforms and the regulatory role of Munc18b J. Cell Sci., June 15, 2008; 121(12): 2097 - 2106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Arunachalam, L. Han, N. G. Tassew, Y. He, L. Wang, L. Xie, Y. Fujita, E. Kwan, B. Davletov, P. P. Monnier, et al. Munc18-1 Is Critical for Plasma Membrane Localization of Syntaxin1 but Not of SNAP-25 in PC12 Cells Mol. Biol. Cell, February 1, 2008; 19(2): 722 - 734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gulyas-Kovacs, H. de Wit, I. Milosevic, O. Kochubey, R. Toonen, J. Klingauf, M. Verhage, and J. B. Sorensen Munc18-1: Sequential Interactions with the Fusion Machinery Stimulate Vesicle Docking and Priming J. Neurosci., August 8, 2007; 27(32): 8676 - 8686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. P.L. Lam, K. Hyvarinen, M. Kauppi, L. Cosen-Binker, S. Laitinen, S. Keranen, H. Y. Gaisano, and V. M. Olkkonen A Cytosolic Splice Variant of Cab45 Interacts with Munc18b and Impacts on Amylase Secretion by Pancreatic Acini Mol. Biol. Cell, July 1, 2007; 18(7): 2473 - 2480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zhang, L. Lilja, S. A. Mandic, J. Gromada, K. Smidt, J. Janson, Y. Takai, C. Bark, P.-O. Berggren, and B. Meister Tomosyn Is Expressed in {beta}-Cells and Negatively Regulates Insulin Exocytosis Diabetes, March 1, 2006; 55(3): 574 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Nicoletta, J. J. Ross, G. Li, Q. Cheng, J. Schwartz, E. A. Alexander, and J. H. Schwartz Munc-18-2 regulates exocytosis of H+-ATPase in rat inner medullary collecting duct cells Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1366 - C1374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Schuck, A. Manninen, M. Honsho, J. Fullekrug, and K. Simons Generation of single and double knockdowns in polarized epithelial cells by retrovirus-mediated RNA interference PNAS, April 6, 2004; 101(14): 4912 - 4917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Serano and G. M. Rubin The Drosophila synaptotagmin-like protein bitesize is required for growth and has mRNA localization sequences within its open reading frame PNAS, November 11, 2003; 100(23): 13368 - 13373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. TUMA and A. L. HUBBARD Transcytosis: Crossing Cellular Barriers Physiol Rev, July 1, 2003; 83(3): 871 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Martin-Verdeaux, I. Pombo, B. Iannascoli, M. Roa, N. Varin-Blank, J. Rivera, and U. Blank Evidence of a role for Munc18-2 and microtubules in mast cell granule exocytosis J. Cell Sci., January 15, 2003; 116(2): 325 - 334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kauppi, G. Wohlfahrt, and V. M. Olkkonen Analysis of the Munc18b-Syntaxin Binding Interface. USE OF A MUTANT Munc18b TO DISSECT THE FUNCTIONS OF SYNTAXINS 2 AND 3 J. Biol. Chem., November 8, 2002; 277(46): 43973 - 43979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, S. H. Low, M. Miura, and T. Weimbs SNARE expression and localization in renal epithelial cells suggest mechanism for variability of trafficking phenotypes Am J Physiol Renal Physiol, November 1, 2002; 283(5): F1111 - F1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rowe, F. Calegari, E. Taverna, R. Longhi, and P. Rosa Syntaxin 1A is delivered to the apical and basolateral domains of epithelial cells: the role of munc-18 proteins J. Cell Sci., March 11, 2002; 114(18): 3323 - 3332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Low, L. Y. Marmorstein, M. Miura, X. Li, N. Kudo, A. D. Marmorstein, and T. Weimbs Retinal pigment epithelial cells exhibit unique expression and localization of plasma membrane syntaxins which may contribute to their trafficking phenotype J. Cell Sci., January 12, 2002; 115(23): 4545 - 4553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ameen, Y Figueroa, and P. Salas Anomalous apical plasma membrane phenotype in CK8-deficient mice indicates a novel role for intermediate filaments in the polarization of simple epithelia J. Cell Sci., January 2, 2001; 114(3): 563 - 575. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Zahraoui, D. Louvard, and T. Galli Tight Junction, a Platform for Trafficking and Signaling Protein Complexes J. Cell Biol., November 20, 2000; 151(5): F31 - F36. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Low, M. Miura, P. A. Roche, A. C. Valdez, K. E. Mostov, and T. Weimbs Intracellular Redirection of Plasma Membrane Trafficking after Loss of Epithelial Cell Polarity Mol. Biol. Cell, September 1, 2000; 11(9): 3045 - 3060. [Abstract] [Full Text]
|
|
|
|
|
|
K. Riento, M. Kauppi, S. Keranen, and V. M. Olkkonen Munc18-2, a Functional Partner of Syntaxin 3, Controls Apical Membrane Trafficking in Epithelial Cells J. Biol. Chem., April 28, 2000; 275(18): 13476 - 13483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tan and S. C. Hooi Syncollin is differentially expressed in rat proximal small intestine and regulated by feeding behavior Am J Physiol Gastrointest Liver Physiol, February 1, 2000; 278(2): G308 - G320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Cabaniols, V. Ravichandran, and P.A. Roche Phosphorylation of SNAP-23 by the Novel Kinase SNAK Regulates t-SNARE Complex Assembly Mol. Biol. Cell, December 1, 1999; 10(12): 4033 - 4041. [Abstract] [Full Text]
|
|
|
|
|
|
S. Saxena, M. W. Quick, A. Tousson, Y. Oh, and D. G. Warnock Interaction of Syntaxins with the Amiloride-sensitive Epithelial Sodium Channel J. Biol. Chem., July 23, 1999; 274(30): 20812 - 20817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Lafont, P. Verkade, T. Galli, C. Wimmer, D. Louvard, and K. Simons Raft association of SNAP receptors acting in apical trafficking in Madin-Darby canine kidney cells PNAS, March 30, 1999; 96(7): 3734 - 3738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B Quinones, K Riento, V. Olkkonen, S Hardy, and M. Bennett Syntaxin 2 splice variants exhibit differential expression patterns, biochemical properties and subcellular localizations J. Cell Sci., January 12, 1999; 112(23): 4291 - 4304. [Abstract] [PDF]
|
|
|
|
|
|
O. Olsen, H. Liu, J. B. Wade, J. Merot, and P. A. Welling Basolateral membrane expression of the Kir 2.3 channel is coordinated by PDZ interaction with Lin-7/CASK complex Am J Physiol Cell Physiol, January 1, 2002; 282(1): C183 - C195. [Abstract] [Full Text] [PDF]
|
|
