|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 17 2689-2696, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
TS Karpova, SL Moltz, LE Riles, U Guldener, JH Hegemann, S Veronneau, H Bussey and JA Cooper
Department of Cell Biology and Physiology, Washington University, St Louis, MO 63110, USA. tkarpova@cellbio.wustl.edu
The yeast actin cytoskeleton is polarized during most of the cell cycle. Certain environmental factors and mutations are associated with depolarization of the actin cytoskeleton. Is depolarization of the actin cytoskeleton a specific response, or is it a nonspecific reaction to harsh conditions or poor metabolism? If depolarization is a nonspecific response, then any mutation that slows growth should induce depolarization. In addition, the number of genes with the depolarization phenotype should constitute a relatively large part of the genome. To address this question, we determined the effect of slow growth on the actin cytoskeleton, and we determined the frequency of mutations that affect the actin cytoskeleton. Eight mutants with slow growth showed no defect in actin polarization, indicating that slow growth alone is not sufficient to cause depolarization. Among 273 viable haploids disrupted for ORFs of chromosome I and VIII and 950 viable haploids with random genome disruptions, none had depolarization of the cytoskeleton. We conclude that depolarization of the actin cytoskeleton is a specific phenotype.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Harsay and R. Schekman Avl9p, a Member of a Novel Protein Superfamily, Functions in the Late Secretory Pathway Mol. Biol. Cell, April 1, 2007; 18(4): 1203 - 1219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Koo, B. Lee, M. E. Young, S. C. Koo, J. A. Cooper, D. Baek, C. O. Lim, S. Y. Lee, D.-J. Yun, and M. J. Cho Pn-AMP1, a Plant Defense Protein, Induces Actin Depolarization in Yeasts Plant Cell Physiol., November 15, 2004; 45(11): 1669 - 1680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Young, J. A. Cooper, and P. C. Bridgman Yeast actin patches are networks of branched actin filaments J. Cell Biol., August 30, 2004; 166(5): 629 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yu and M. Cai The yeast dynamin-related GTPase Vps1p functions in the organization of the actin cytoskeleton via interaction with Sla1p J. Cell Sci., August 1, 2004; 117(17): 3839 - 3853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Zakrzewska, M. Perron, A. Laroche, and D. Pallotta A Role for GEA1 and GEA2 in the Organization of the Actin Cytoskeleton in Saccharomyces cerevisiae Genetics, November 1, 2003; 165(3): 985 - 995. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Torres, C. J. Di Como, E. Herrero, and M. A. de la Torre-Ruiz Regulation of the Cell Integrity Pathway by Rapamycin-sensitive TOR Function in Budding Yeast J. Biol. Chem., November 1, 2002; 277(45): 43495 - 43504. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Young, T. S. Karpova, B. Brugger, D. M. Moschenross, G. K. Wang, R. Schneiter, F. T. Wieland, and J. A. Cooper The Sur7p Family Defines Novel Cortical Domains in Saccharomyces cerevisiae, Affects Sphingolipid Metabolism, and Is Involved in Sporulation Mol. Cell. Biol., February 1, 2002; 22(3): 927 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Smith, S. Swamy, and L. Pon The life cycle of actin patches in mating yeast J. Cell Sci., January 4, 2001; 114(8): 1505 - 1513. [Abstract] [PDF]
|
|
|
|
|
|
T. S. Karpova, S. L. Reck-Peterson, N. B. Elkind, M. S. Mooseker, P. J. Novick, and J. A. Cooper Role of Actin and Myo2p in Polarized Secretion and Growth of Saccharomyces cerevisiae Mol. Biol. Cell, May 1, 2000; 11(5): 1727 - 1737. [Abstract] [Full Text]
|
|
|
|
|
|
R. G. Spelbrink and S. F. Nothwehr The Yeast GRD20 Gene Is Required for Protein Sorting in the trans-Golgi Network/Endosomal System and for Polarization of the Actin Cytoskeleton Mol. Biol. Cell, December 1, 1999; 10(12): 4263 - 4281. [Abstract] [Full Text]
|
|
