|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 18 2779-2788, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
HE Stoffler, U Honnert, CA Bauer, D Hofer, H Schwarz, RT Muller, D Drenckhahn and M Bahler
Adolf-Butenandt-Institute, Cell Biology, Ludwig-Maximilians-University, D-80336 Munich, Germany.
Myr 3, a member of the myosin-I family from rat, is shown in this study to be localized at adherens-type intercellular junctions in epithelial and nonepithelial tissues. Formation of intercellular junctions and the accompanying recruitment of myr 3 to these junctions involves signaling by the Rho subfamily of small GTP-binding proteins. This conclusion is based on studies with HtTA-1 HeLa cells that were induced by overexpression of constitutively active Cdc42Hs to form typical adherens-type intercellular junctions enriched in cadherins (N-cadherin), beta-catenin, filamentous actin and myr 3. Recruitement of myr 3 to Cdc42-induced adherens junctions in HeLa cells was dependent on a short region of the tail domain and a functional myosin motor domain, but was independent of its myosin-I tail homology and SH3 regions. Overexpression of constitutively active Rac1 induced a distinct type of adherens junction in HeLa cells that was characterized by elaborate intercellular interdigitations enriched in N-cadherin, beta-catenin and F-actin. Myr 3 was often present, but not specifically enriched in the intercellular junctions induced by constitutively active Rac1.
This article has been cited by other articles:
|
|
 |
|
  C. Schietroma, H.-Y. Yu, M. C. Wagner, J. A. Umbach, W. M. Bement, and C. B. Gundersen A Role for Myosin 1e in Cortical Granule Exocytosis in Xenopus Oocytes J. Biol. Chem., October 5, 2007; 282(40): 29504 - 29513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, M. Betson, J. Erasmus, K. Zeikos, M. Bailly, L. P. Cramer, and V. M. M. Braga Actin at cell-cell junctions is composed of two dynamic and functional populations J. Cell Sci., December 1, 2005; 118(23): 5549 - 5562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Mezgueldi, N. Tang, S. S. Rosenfeld, and E. M. Ostap The Kinetic Mechanism of Myo1e (Human Myosin-IC) J. Biol. Chem., June 7, 2002; 277(24): 21514 - 21521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B Wojciak-Stothard, S Potempa, T Eichholtz, and A. Ridley 9Rgr; and Rac but not Cdc42 regulate endothelial cell permeability J. Cell Sci., January 4, 2001; 114(7): 1343 - 1355. [Abstract] [PDF]
|
|
|
|
 |
|
 Y. Takai, T. Sasaki, and T. Matozaki Small GTP-Binding Proteins Physiol Rev, January 1, 2001; 81(1): 153 - 208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Hirsch, D. M. Pirone, and P. D. Burbelo A New Family of Cdc42 Effector Proteins, CEPs, Function in Fibroblast and Epithelial Cell Shape Changes J. Biol. Chem., January 5, 2001; 276(2): 875 - 883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Senda, S.-F. Lee, G. P. Cote, and M. A. Titus Recruitment of a Specific Amoeboid Myosin I Isoform to the Plasma Membrane in Chemotactic Dictyostelium Cells J. Biol. Chem., January 19, 2001; 276(4): 2898 - 2904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Wei and R. S. Adelstein Pitx2a Expression Alters Actin-Myosin Cytoskeleton and Migration of HeLa Cells through Rho GTPase Signaling Mol. Biol. Cell, February 1, 2002; 13(2): 683 - 697. [Abstract] [Full Text] [PDF]
|
|
