|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 20 3045-3057, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
AP Kowalczyk, P Navarro, E Dejana, EA Bornslaeger, KJ Green, DS Kopp and JE Borgwardt
Departments of Dermatology, Pathology, and The Robert H. Lurie Cancer Center, Northwestern University Medical School, Chicago, IL, USA. akowalc@emory.edu.
Vascular endothelial cells assemble adhesive intercellular junctions comprising a unique cadherin, VE-cadherin, which is coupled to the actin cytoskeleton through cytoplasmic interactions with plakoglobin, beta-catenin and alpha -catenin. However, the potential linkage between VE-cadherin and the vimentin intermediate filament cytoskeleton is not well characterized. Recent evidence indicates that lymphatic and vascular endothelial cells express desmoplakin, a cytoplasmic desmosomal protein that attaches intermediate filaments to the plasma membrane in epithelial cells. In the present study, desmoplakin was localized to intercellular junctions in human dermal microvascular endothelial cells. To determine if VE-cadherin could associate with desmoplakin, VE-cadherin, plakoglobin, and a desmoplakin amino-terminal polypeptide (DP-NTP) were co-expressed in L-cell fibroblasts. In the presence of VE-cadherin, both plakoglobin and DP-NTP were recruited to cell-cell borders. Interestingly, beta-catenin could not substitute for plakoglobin in the recruitment of DP-NTP to cell borders, and DP-NTP bound to plakoglobin but not beta-catenin in the yeast two-hybrid system. In addition, DP-NTP colocalized at cell-cell borders with alpha-catenin in the L-cell lines, and endogenous desmoplakin and alpha-catenin colocalized in cultured dermal microvascular endothelial cells. This is in striking contrast to epithelial cells, where desmoplakin and alpha -+catenin are restricted to desmosomes and adherens junctions, respectively. These results suggest that endothelial cells assemble unique junctional complexes that couple VE-cadherin to both the actin and intermediate filament cytoskeleton.
This article has been cited by other articles:
|
|
 |
|
  S. Kim and P. A. Coulombe Intermediate filament scaffolds fulfill mechanical, organizational, and signaling functions in the cytoplasm Genes & Dev., July 1, 2007; 21(13): 1581 - 1597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Liwosz, T. Lei, and M. A. Kukuruzinska N-Glycosylation Affects the Molecular Organization and Stability of E-cadherin Junctions J. Biol. Chem., August 11, 2006; 281(32): 23138 - 23149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Xiao, J. Garner, K. M. Buckley, P. A. Vincent, C. M. Chiasson, E. Dejana, V. Faundez, and A. P. Kowalczyk p120-Catenin Regulates Clathrin-dependent Endocytosis of VE-Cadherin Mol. Biol. Cell, November 1, 2005; 16(11): 5141 - 5151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. J. Kim, C. Sauer, K. Testa, J. K. Wahl, R. A. Svoboda, K. R. Johnson, M. J. Wheelock, and K. A. Knudsen Modulating the strength of cadherin adhesion: evidence for a novel adhesion complex J. Cell Sci., September 1, 2005; 118(17): 3883 - 3894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Tzima, J. S. Reader, M. Irani-Tehrani, K. L. Ewalt, M. A. Schwartz, and P. Schimmel VE-cadherin Links tRNA Synthetase Cytokine to Anti-angiogenic Function J. Biol. Chem., January 28, 2005; 280(4): 2405 - 2408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Grossmann, C. Grund, J. Huelsken, M. Behrend, B. Erdmann, W. W. Franke, and W. Birchmeier Requirement of plakophilin 2 for heart morphogenesis and cardiac junction formation J. Cell Biol., October 11, 2004; 167(1): 149 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bazzoni and E. Dejana Endothelial Cell-to-Cell Junctions: Molecular Organization and Role in Vascular Homeostasis Physiol Rev, July 1, 2004; 84(3): 869 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhou, A. Stuart, L. E. Dettin, G. Rodriguez, B. Hoel, and G. I. Gallicano Desmoplakin is required for microvascular tube formation in culture J. Cell Sci., July 1, 2004; 117(15): 3129 - 3140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Vincent, K. Xiao, K. M. Buckley, and A. P. Kowalczyk VE-cadherin: adhesion at arm's length Am J Physiol Cell Physiol, May 1, 2004; 286(5): C987 - C997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Xiao, D. F. Allison, K. M. Buckley, M. D. Kottke, P. A. Vincent, V. Faundez, and A. P. Kowalczyk Cellular levels of p120 catenin function as a set point for cadherin expression levels in microvascular endothelial cells J. Cell Biol., November 10, 2003; 163(3): 535 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hatsell, L. Medina, J. Merola, R. Haltiwanger, and P. Cowin Plakoglobin Is O-Glycosylated Close to the N-terminal Destruction Box J. Biol. Chem., September 26, 2003; 278(39): 37745 - 37752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cattelino, S. Liebner, R. Gallini, A. Zanetti, G. Balconi, A. Corsi, P. Bianco, H. Wolburg, R. Moore, B. Oreda, et al. The conditional inactivation of the {beta}-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility J. Cell Biol., September 15, 2003; 162(6): 1111 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Young, X. Sui, T. D. Kiser, S. W. Hyun, P. Wang, S. Sakarya, D. J. Angelini, K. L. Schaphorst, J. D. Hasday, A. S. Cross, et al. Protein tyrosine phosphatase activity regulates endothelial cell-cell interactions, the paracellular pathway, and capillary tube stability Am J Physiol Lung Cell Mol Physiol, July 1, 2003; 285(1): L63 - L75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Xiao, D. F. Allison, M. D. Kottke, S. Summers, G. P. Sorescu, V. Faundez, and A. P. Kowalczyk Mechanisms of VE-cadherin Processing and Degradation in Microvascular Endothelial Cells J. Biol. Chem., May 23, 2003; 278(21): 19199 - 19208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. F. Davies, J. Zilberberg, and B. P. Helmke Spatial Microstimuli in Endothelial Mechanosignaling Circ. Res., March 7, 2003; 92(4): 359 - 370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Calkins, B. L. Hoepner, C. M. Law, M. R. Novak, S. V. Setzer, M. Hatzfeld, and A. P. Kowalczyk The Armadillo Family Protein p0071 Is a VE-cadherin- and Desmoplakin-binding Protein J. Biol. Chem., January 10, 2003; 278(3): 1774 - 1783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Venkiteswaran, K. Xiao, S. Summers, C. C. Calkins, P. A. Vincent, K. Pumiglia, and A. P. Kowalczyk Regulation of endothelial barrier function and growth by VE-cadherin, plakoglobin, and beta -catenin Am J Physiol Cell Physiol, September 1, 2002; 283(3): C811 - C821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bibert, M. Jaquinod, E. Concord, C. Ebel, E. Hewat, C. Vanbelle, P. Legrand, M. Weidenhaupt, T. Vernet, and D. Gulino-Debrac Synergy between Extracellular Modules of Vascular Endothelial Cadherin Promotes Homotypic Hexameric Interactions J. Biol. Chem., April 5, 2002; 277(15): 12790 - 12801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Johnson and K. Boekelheide Dynamic Testicular Adhesion Junctions Are Immunologically Unique. II. Localization of Classic Cadherins in Rat Testis Biol Reprod, April 1, 2002; 66(4): 992 - 1000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Chen, S. Bonne, M. Hatzfeld, F. van Roy, and K. J. Green Protein Binding and Functional Characterization of Plakophilin 2. EVIDENCE FOR ITS DIVERSE ROLES IN DESMOSOMES AND beta -CATENIN SIGNALING J. Biol. Chem., March 15, 2002; 277(12): 10512 - 10522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gallicano, C Bauer, and E Fuchs Rescuing desmoplakin function in extra-embryonic ectoderm reveals the importance of this protein in embryonic heart, neuroepithelium, skin and vasculature Development, January 3, 2001; 128(6): 929 - 941. [Abstract] [PDF]
|
|
|
|
|
|
B. Angst, C Marcozzi, and A. Magee The cadherin superfamily: diversity in form and function J. Cell Sci., January 2, 2001; 114(4): 629 - 641. [Abstract] [PDF]
|
|
|
|
|
|
E. Bornslaeger, L. Godsel, C. Corcoran, J. Park, M Hatzfeld, A. Kowalczyk, and K. Green Plakophilin 1 interferes with plakoglobin binding to desmoplakin, yet together with plakoglobin promotes clustering of desmosomal plaque complexes at cell-cell borders J. Cell Sci., January 2, 2001; 114(4): 727 - 738. [Abstract] [PDF]
|
|
|
|
|
|
S. Noria, D. B. Cowan, A. I. Gotlieb, and B. L. Langille Transient and Steady-State Effects of Shear Stress on Endothelial Cell Adherens Junctions Circ. Res., September 17, 1999; 85(6): 504 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Kowalczyk, M. Hatzfeld, E. A. Bornslaeger, D. S. Kopp, J. E. Borgwardt, C. M. Corcoran, A. Settler, and K. J. Green The Head Domain of Plakophilin-1 Binds to Desmoplakin and Enhances Its Recruitment to Desmosomes. IMPLICATIONS FOR CUTANEOUS DISEASE J. Biol. Chem., June 25, 1999; 274(26): 18145 - 18148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. North, W. Bardsley, J Hyam, E. Bornslaeger, H. Cordingley, B Trinnaman, M Hatzfeld, K. Green, A. Magee, and D. Garrod Molecular map of the desmosomal plaque J. Cell Sci., January 12, 1999; 112(23): 4325 - 4336. [Abstract] [PDF]
|
|
|
|
|
|
N. Ilan, L. Cheung, E. Pinter, and J. A. Madri Platelet-Endothelial Cell Adhesion Molecule-1 (CD31), a Scaffolding Molecule for Selected Catenin Family Members Whose Binding Is Mediated by Different Tyrosine and Serine/Threonine Phosphorylation J. Biol. Chem., July 7, 2000; 275(28): 21435 - 21443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Shasby, D. R. Ries, S. S. Shasby, and M. C. Winter Histamine stimulates phosphorylation of adherens junction proteins and alters their link to vimentin Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1330 - L1338. [Abstract] [Full Text] [PDF]
|
|
