|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 20 3059-3071, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
J Faix, C Clougherty, A Konzok, U Mintert, J Murphy, R Albrecht, B Muhlbauer and J Kuhlmann
Max-Planck-Institut fur Biochemie, D-82152 Martinsried, Germany. faix@biochem.mpg.de.
DGAP1 of Dictyostelium discoideum is a cell cortex associated 95 kDa protein that shows homology to both RasGTPase-activating proteins (RasGAPs) and RasGAP-related proteins. When tested for RasGAP activity, recombinant DGAP1 protein did not promote the GTPase activity of human H-Ras or of Dictyostelium RasG in vitro. Instead, DGAP1 bound to Dictyostelium Rac1A and human Rac1, but not to human Cdc42. DGAP1 preferentially interacted with the activated GTP-bound forms of Rac1 and Rac1A, but did not affect the GTPase activities. Since Rho-type GTPases are implicated in the formation of specific F-actin structures and in the control of cell morphology, the microfilament system of mutants that either lack or overexpress DGAP1 has been analysed. DGAP1-null mutants showed elevated levels of F-actin that was organised in large leading edges, membrane ruffles or numerous large filopods. Expression of actin fused to green fluorescent protein (GFP) was used to monitor the actin dynamics in these cells, and revealed that the F-actin cytoskeleton of DGAP1-null cells was rapidly re-arranged to form ruffles and filopods. Conversely, in DGAP1-overexpressing cells, the formation of cellular projections containing F-actin was largely suppressed. Measurement of cell migration demonstrated that DGAP1 expression is inversely correlated with the speed of cell motility.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  R. J. W. Heath and R. H. Insall Dictyostelium MEGAPs: F-BAR domain proteins that regulate motility and membrane tubulation in contractile vacuoles J. Cell Sci., April 1, 2008; 121(7): 1054 - 1064. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rehberg, J. Kleylein-Sohn, J. Faix, T.-H. Ho, I. Schulz, and R. Graf Dictyostelium LIS1 Is a Centrosomal Protein Required for Microtubule/Cell Cortex Interactions, Nucleus/Centrosome Linkage, and Actin Dynamics Mol. Biol. Cell, June 1, 2005; 16(6): 2759 - 2771. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. N. Robinson, S. S. Ocon, R. S. Rock, and J. A. Spudich Dynacortin Is a Novel Actin Bundling Protein That Localizes to Dynamic Actin Structures J. Biol. Chem., March 8, 2002; 277(11): 9088 - 9095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Davy, H. Campbell, S Fountain, D de Jong, and M. Crouch The flightless I protein colocalizes with actin- and microtubule-based structures in motile Swiss 3T3 fibroblasts: evidence for the involvement of PI 3-kinase and Ras-related small GTPases J. Cell Sci., January 2, 2001; 114(3): 549 - 562. [Abstract] [PDF]
|
|
|
|
|
|
M Dumontier, P Hocht, U Mintert, and J Faix Rac1 GTPases control filopodia formation, cell motility, endocytosis, cytokinesis and development in Dictyostelium J. Cell Sci., January 6, 2000; 113(12): 2253 - 2265. [Abstract] [PDF]
|
|
|
|
|
|
A. Noegel and M Schleicher The actin cytoskeleton of Dictyostelium: a story told by mutants J. Cell Sci., January 3, 2000; 113(5): 759 - 766. [Abstract] [PDF]
|
|
|
|
|
|
J. Chubb, A Wilkins, G. Thomas, and R. Insall The Dictyostelium RasS protein is required for macropinocytosis, phagocytosis and the control of cell movement J. Cell Sci., January 2, 2000; 113(4): 709 - 719. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Y. Chung and R. A. Firtel Paka, a Putative Pak Family Member, Is Required for Cytokinesis and the Regulation of the Cytoskeleton in Dictyostelium discoideum Cells during Chemotaxis J. Cell Biol., November 1, 1999; 147(3): 559 - 576. [Abstract] [Full Text] [PDF]
|
|
