spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fages, C.
Right arrow Articles by Rauvala, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fages, C.
Right arrow Articles by Rauvala, H.

Journal of Cell Science, Vol 111, Issue 20 3073-3080, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

Regulation of mRNA localization by transmembrane signalling: local interaction of HB-GAM (heparin-binding growth-associated molecule) with the cell surface localizes beta-actin mRNA

C Fages, M Kaksonen, T Kinnunen, EL Punnonen and H Rauvala
Laboratory of Molecular Neurobiology, Institute of Biotechnology, the Division of Biochemistry, Department of Biosciences, and Electron Microscopy Unit, Institute of Biotechnology, FIN-00014 University of Helsinki, Finland.

Localization of mRNAs is currently thought to be partially responsible for molecular sorting to specific compartments within the cell. In mammalian cells the best-studied example is the beta-actin mRNA that is localized to the cell processes, and its localization is necessary in migratory responses of cells. It is reasonable to assume that mRNA localization within cells is coupled to transmembrane signalling due to extracellular factors, but little is known about such putative mechanisms. We show here that HB-GAM, an extracellular matrix-associated factor that enhances migratory responses in cells, is able to localize beta-actin mRNA when locally applied to cells via microbeads. The HB-GAM-induced mRNA localization is specifically inhibited by low concentrations of heparin and by heparitinase treatment of cells, showing that cell-surface heparin-type glycans are required for the effect. The finding that soluble N-syndecan is also inhibitory suggests that the transmembrane proteoglycan N-syndecan, previously identified as an HB-GAM receptor, is involved in the mRNA-localizing effect of HB-GAM. Inhibition of the mRNA localization by the src-kinase inhibitor PP1 is compatible with an N-syndecan-mediated effect since the receptor function of N-syndecan has been recently found to depend on the src-kinase signalling pathway. The mRNA-localizing activity of N-syndecan is also suggested by the finding that affinity-purified anti-N-syndecan antibodies coated on microbeads are able to localize beta-actin mRNA.


This article has been cited by other articles:


Home page
 IOVSHome page
M. Inatani, M. Honjo, A. Oohira, N. Kido, Y. Otori, Y. Tano, Y. Honda, and H. Tanihara
Spatiotemporal Expression Patterns of N-Syndecan, a Transmembrane Heparan Sulfate Proteoglycan, in Developing Retina
Invest. Ophthalmol. Vis. Sci., May 1, 2002; 43(5): 1616 - 1621.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C Fages, R Nolo, H. Huttunen, E Eskelinen, and H Rauvala
Regulation of cell migration by amphoterin
J. Cell Sci., January 2, 2000; 113(4): 611 - 620.
[Abstract] [PDF]

Home page
 J. Neurosci.Home page
S. E. Lauri, S. Kaukinen, T. Kinnunen, A. Ylinen, S. Imai, K. Kaila, T. Taira, and H. Rauvala
Reg1ulatory Role and Molecular Interactions of a Cell-Surface Heparan Sulfate Proteoglycan (N-syndecan) in Hippocampal Long-Term Potentiation
J. Neurosci., February 15, 1999; 19(4): 1226 - 1235.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1998