Journal of Cell Science, Vol 111, Issue 20 3091-3100, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

A cell cycle regulated MAP kinase with a possible role in cytokinesis in tobacco cells

O Calderini, L Bogre, O Vicente, P Binarova, E Heberle-Bors and C Wilson
Institute of Microbiology and Genetics, University of Vienna, Dr Bohrgasse 9, A-1030 Vienna, Austria.

Mitogen-activated protein (MAP) kinases have been demonstrated to have a role in meiosis but their involvement in mitotic events is less clear. Using a peptide antibody raised against the tobacco MAP kinase p43(Ntf6) and extracts from synchronized tobacco cell suspension cultures, we show that this kinase is activated specifically during mitosis. Entry into mitosis appears to be necessary for the activation of the kinase, which occurs as a post-translational event. The activation of the kinase occurs in late anaphase/early telophase. The p43(Ntf6) protein shows a transient localization to the cell plate in anaphase cells, in the middle of the two microtubule arrays characteristic of the phragmoplast, a plant-specific structure involved in laying down the new cell wall. The combined data support a role for the MAP kinase p43(Ntf6) in cytokinesis in tobacco cells.

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