|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 20 3101-3108, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
E Greenwood, H Nishitani and P Nurse
Cell Cycle Laboratory, Imperial Cancer Research Fund, London, WC2A 3PX, UK. e.greenwood@icrf.icnet.uk.
The DNA replication checkpoint is required to maintain the integrity of the genome, inhibiting mitosis until S phase has been successfully completed. The checkpoint preventing premature mitosis in Schizosaccharomyces pombe relies on phosphorylation of the tyrosine-15 residue on cdc2p to prevent its activation and hence mitosis. The cdc18 gene is essential for both generating the DNA replication checkpoint and the initiation of S phase, thus providing a key role for the overall control and coordination of the cell cycle. We show that the C terminus of the protein is capable of both initiating DNA replication and the checkpoint function of cdc18p. The C terminus of cdc18p acts upstream of the DNA replication checkpoint genes rad1, rad3, rad9, rad17, hus1 and cut5 and requires the wee1p/mik1p tyrosine kinases to block mitosis. The N terminus of cdc18p can also block mitosis but does so in the absence of the DNA replication checkpoint genes and the wee1p/mik1p kinases therefore acting downstream of these genes. Because the N terminus of cdc18p associates with cdc2p in vivo, we suggest that by binding the cdc2p/cdc13p mitotic kinase directly, it exerts an effect independently of the normal checkpoint control, probably in an unphysiological manner.
This article has been cited by other articles:
|
|
 |
|
  N. Fersht, D. Hermand, J. Hayles, and P. Nurse Cdc18/CDC6 activates the Rad3-dependent checkpoint in the fission yeast Nucleic Acids Res., August 13, 2007; 35(16): 5323 - 5337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Boronat and J. L. Campbell Mitotic Cdc6 Stabilizes Anaphase-Promoting Complex Substrates by a Partially Cdc28-Independent Mechanism, and This Stabilization Is Suppressed by Deletion of Cdc55 Mol. Cell. Biol., February 1, 2007; 27(3): 1158 - 1171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Chen, L. Calzone, A. Csikasz-Nagy, F. R. Cross, B. Novak, and J. J. Tyson Integrative Analysis of Cell Cycle Control in Budding Yeast Mol. Biol. Cell, August 1, 2004; 15(8): 3841 - 3862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Synnes, E. A. Nilssen, E. Boye, and B. Grallert A novel chk1-dependent G1/M checkpoint in fission yeast J. Cell Sci., September 15, 2002; 115(18): 3609 - 3618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Gopalakrishnan, P. Simancek, C. Houchens, H. A. Snaith, M. G. Frattini, S. Sazer, and T. J. Kelly Redundant control of rereplication in fission yeast PNAS, October 16, 2001; (2001) 221467598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tournier and J. B.A. Millar A Role for the START Gene-specific Transcription Factor Complex in the Inactivation of Cyclin B and Cut2 Destruction Mol. Biol. Cell, October 1, 2000; 11(10): 3411 - 3424. [Abstract] [Full Text]
|
|
|
|
|
|
J. Raleigh and M. O'Connell The G(2) DNA damage checkpoint targets both Wee1 and Cdc25 J. Cell Sci., January 5, 2000; 113(10): 1727 - 1736. [Abstract] [PDF]
|
|
|
|
|
|
C. P. C. De Souza, X. S. Ye, and S. A. Osmani Checkpoint Defects Leading to Premature Mitosis Also Cause Endoreplication of DNA in Aspergillus nidulans Mol. Biol. Cell, November 1, 1999; 10(11): 3661 - 3674. [Abstract] [Full Text]
|
|
|
|
|
|
V. Gopalakrishnan, P. Simancek, C. Houchens, H. A. Snaith, M. G. Frattini, S. Sazer, and T. J. Kelly Redundant control of rereplication in fission yeast PNAS, November 6, 2001; 98(23): 13114 - 13119. [Abstract] [Full Text] [PDF]
|
|
