|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 21 3179-3188, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
S Lyle, M Christofidou-Solomidou, Y Liu, DE Elder, S Albelda and G Cotsarelis
University of Pennsylvania Medical Center, Departments of Pathology and Laboratory Medicine, Medicine and Dermatology, Philadelphia, PA 19104, USA.
Stem cells are vital for the homeostasis of self-renewing tissues such as the hair follicle. Epithelial stem cells have been implicated in tumorigenesis and wound healing, and their manipulation may have wide ranging applications including gene therapy and tissue transplantation. Rodent hair follicle stem cells have been localized to an area of the follicle called the bulge, however, the identification and characterization of human hair follicle stem cells has been hampered by a lack of cellular markers for this area. We have determined that the C8/144B monoclonal antibody, originally generated against a short intracytoplasmic peptide of CD8, preferentially immunostains hair follicle bulge keratinocytes without staining the remaining hair follicle. Using expression cloning, we identified cytokeratin 15 as the keratinocyte protein recognized by the C8/144B monoclonal antibody. By delineating the bulge using this antibody, we demonstrated that bulge cells possess a stem cell phenotype characterized by their slowly-cycling nature, preferential proliferation at the onset of new hair follicle growth, high level of beta1 integrin expression, and expression of cytokeratin 19.
This article has been cited by other articles:
|
|
 |
|
  D. Larouche, X. Tong, J. Fradette, P. A. Coulombe, and L. Germain Vibrissa hair bulge houses two populations of skin epithelial stem cells distinct by their keratin profile FASEB J, May 1, 2008; 22(5): 1404 - 1415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A Randall, T. J Jenner, N. A Hibberts, I. O De Oliveira, and T. Vafaee Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles J. Endocrinol., April 1, 2008; 197(1): 11 - 23. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kadoya, J.-i. Fukushi, Y. Matsumoto, Y. Yamaguchi, and W. B. Stallcup NG2 Proteoglycan Expression in Mouse Skin: Altered Postnatal Skin Development in the NG2 Null Mouse J. Histochem. Cytochem., March 1, 2008; 56(3): 295 - 303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Crigler, A. Kazhanie, T.-J. Yoon, J. Zakhari, J. Anders, B. Taylor, and V. M. Virador Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages FASEB J, July 1, 2007; 21(9): 2050 - 2063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Figueira, N. Di Girolamo, M. T. Coroneo, and D. Wakefield The Phenotype of Limbal Epithelial Stem Cells Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 144 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. F. Hu, Z.-J. Zhang, and M. Sieber-Blum An Epidermal Neural Crest Stem Cell (EPI-NCSC) Molecular Signature Stem Cells, December 1, 2006; 24(12): 2692 - 2702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kazantseva, A. Goltsov, R. Zinchenko, A. P. Grigorenko, A. V. Abrukova, Y. K. Moliaka, A. G. Kirillov, Z. Guo, S. Lyle, E. K. Ginter, et al. Human Hair Growth Deficiency Is Linked to a Genetic Defect in the Phospholipase Gene LIPH. Science, November 10, 2006; 314(5801): 982 - 985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yoshida, S. Shimmura, T. Kawakita, H. Miyashita, S. Den, J. Shimazaki, and K. Tsubota Cytokeratin 15 Can Be Used to Identify the Limbal Phenotype in Normal and Diseased Ocular Surfaces Invest. Ophthalmol. Vis. Sci., November 1, 2006; 47(11): 4780 - 4786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Adachi, H. Ulanovsky, Y. Li, B. Norman, J. Davis, and J. Piatigorsky Serial Analysis of Gene Expression (SAGE) in the Rat Limbal and Central Corneal Epithelium. Invest. Ophthalmol. Vis. Sci., September 1, 2006; 47(9): 3801 - 3810. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. W. Nijhof, K. M. Braun, A. Giangreco, C. van Pelt, H. Kawamoto, R. L. Boyd, R. Willemze, L. H. F. Mullenders, F. M. Watt, F. R. de Gruijl, et al. The cell-surface marker MTS24 identifies a novel population of follicular keratinocytes with characteristics of progenitor cells Development, August 1, 2006; 133(15): 3027 - 3037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yu, D. Fang, S. M. Kumar, L. Li, T. K. Nguyen, G. Acs, M. Herlyn, and X. Xu Isolation of a Novel Population of Multipotent Adult Stem Cells from Human Hair Follicles Am. J. Pathol., June 1, 2006; 168(6): 1879 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Zcharia, D. Philp, E. Edovitsky, H. Aingorn, S. Metzger, H. K. Kleinman, I. Vlodavsky, and M. Elkin Heparanase Regulates Murine Hair Growth Am. J. Pathol., April 1, 2005; 166(4): 999 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Roh, Q. Tao, and S. Lyle Dermal papilla-induced hair differentiation of adult epithelial stem cells from human skin Physiol Genomics, October 4, 2004; 19(2): 207 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Legg, U. B. Jensen, S. Broad, I. Leigh, and F. M. Watt Role of melanoma chondroitin sulphate proteoglycan in patterning stem cells in human interfollicular epidermis Development, December 15, 2003; 130(24): 6049 - 6063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Braun, C. Niemann, U. B. Jensen, J. P. Sundberg, V. Silva-Vargas, and F. M. Watt Manipulation of stem cell proliferation and lineage commitment: visualisation of label-retaining cells in wholemounts of mouse epidermis Development, November 1, 2003; 130(21): 5241 - 5255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kataoka, R. J. Medina, T. Kageyama, M. Miyazaki, T. Yoshino, T. Makino, and N.-h. Huh Participation of Adult Mouse Bone Marrow Cells in Reconstitution of Skin Am. J. Pathol., October 1, 2003; 163(4): 1227 - 1231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Xu, S. Lyle, Y. Liu, B. Solky, and G. Cotsarelis Differential Expression of Cyclin D1 in the Human Hair Follicle Am. J. Pathol., September 1, 2003; 163(3): 969 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Tunggal, J. Ravaux, M. Pesch, H. Smola, T. Krieg, F. Gaill, T. Sasaki, R. Timpl, C. Mauch, and M. Aumailley Defective Laminin 5 Processing in Cylindroma Cells Am. J. Pathol., February 1, 2002; 160(2): 459 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Borthwick, M. Shahbazian, Q. Todd Krantz, J. R. Dorin, and S. H. Randell Evidence for Stem-Cell Niches in the Tracheal Epithelium Am. J. Respir. Cell Mol. Biol., June 1, 2001; 24(6): 662 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. V. BOTCHKAREVA, M. KHLGATIAN, B. J. LONGLEY, V. A. BOTCHKAREV, and B. A. GILCHREST SCF/c-kit signaling is required for cyclic regeneration of the hair pigmentation unit FASEB J, March 1, 2001; 15(3): 645 - 658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Panteleyev, C. A. B. Jahoda, and A. M. Christiano Hair follicle predetermination J. Cell Sci., January 10, 2001; 114(19): 3419 - 3431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Stenn and R. Paus Controls of Hair Follicle Cycling Physiol Rev, January 1, 2001; 81(1): 449 - 494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wu, Z. H. Wang, A. Yan, S. Lyle, S. Fakharzadeh, J. K. Wahl, M. J. Wheelock, H. Ishikawa, J. Uitto, M. Amagai, et al. Protection against Pemphigus Foliaceus by Desmoglein 3 in Neonates N. Engl. J. Med., July 6, 2000; 343(1): 31 - 35. [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Fakharzadeh, Y. Zhang, R. Sarkar, and H. H. Kazazian Jr Correction of the coagulation defect in hemophilia A mice through factor VIII expression in skin Blood, May 1, 2000; 95(9): 2799 - 2805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Slack Stem Cells in Epithelial Tissues Science, February 25, 2000; 287(5457): 1431 - 1433. [Abstract] [Full Text]
|
|
|
|
|
|
R. Paus and G. Cotsarelis The Biology of Hair Follicles N. Engl. J. Med., August 12, 1999; 341(7): 491 - 497. [Full Text] [PDF]
|
|
