|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 22 3333-3346, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
O Gavet, S Ozon, V Manceau, S Lawler, P Curmi and A Sobel
INSERM U440, IFM, 75005 Paris, France.
Stathmin is a small regulatory phosphoprotein integrating diverse intracellular signaling pathways. It is also the generic element of a protein family including the neural proteins SCG10, SCLIP, RB3 and its two splice variants RB3' and RB3". Stathmin itself was shown to interact in vitro with tubulin in a phosphorylation-dependent manner, sequestering free tubulin and hence promoting microtubule depolymerization. We investigated the intracellular distribution and tubulin depolymerizing activity in vivo of all known members of the stathmin family. Whereas stathmin is not associated with interphase microtubules in HeLa cells, a fraction of it is concentrated at the mitotic spindle. We generated antisera specific for stathmin phosphoforms, which allowed us to visualize the regulation of phosphorylation-dephosphorylation during the successive stages of mitosis, and the partial localization of stathmin phosphorylated on serine 16 at the mitotic spindle. Results from overexpression experiments of wild-type and novel phosphorylation site mutants of stathmin further suggest that it induces depolymerization of interphase and mitotic microtubules in its unphosphorylated state but is inactivated by phosphorylation in mitosis. Phosphorylation of mutants 16A25A and 38A63A on sites 38 and 63 or 16 and 25, respectively, was sufficient for the formation of a functional spindle, whereas mutant 16A25A38A63E retained a microtubule depolymerizing activity. Transient expression of each of the neural phosphoproteins of the stathmin family showed that they are at least partially associated to the Golgi apparatus and not to other major membrane compartments, probably through their different NH2-terminal domains, as described for SCG10. Most importantly, like stathmin and SCG10, overexpressed SCLIP, RB3 and RB3" were able to depolymerize interphase microtubules. Altogether, our results demonstrate in vivo the functional conservation of the stathmin domain within each protein of the stathmin family, with a microtubule destabilizing activity most likely essential for their specific biological function(s).
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Manna, D. A. Thrower, S. Honnappa, M. O. Steinmetz, and L. Wilson Regulation of Microtubule Dynamic Instability in Vitro by Differentially Phosphorylated Stathmin J. Biol. Chem., June 5, 2009; 284(23): 15640 - 15649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Singer, M. Malz, E. Herpel, A. Warth, M. Bissinger, M. Keith, T. Muley, M. Meister, H. Hoffmann, R. Penzel, et al. Coordinated Expression of Stathmin Family Members by Far Upstream Sequence Element-Binding Protein-1 Increases Motility in Non-Small Cell Lung Cancer Cancer Res., March 15, 2009; 69(6): 2234 - 2243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Belletti, M. S. Nicoloso, M. Schiappacassi, S. Berton, F. Lovat, K. Wolf, V. Canzonieri, S. D'Andrea, A. Zucchetto, P. Friedl, et al. Stathmin Activity Influences Sarcoma Cell Shape, Motility, and Metastatic Potential Mol. Biol. Cell, May 1, 2008; 19(5): 2003 - 2013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Pandithage, R. Lilischkis, K. Harting, A. Wolf, B. Jedamzik, J. Luscher-Firzlaff, J. Vervoorts, E. Lasonder, E. Kremmer, B. Knoll, et al. The regulation of SIRT2 function by cyclin-dependent kinases affects cell motility J. Cell Biol., March 5, 2008; 180(5): 915 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ohkawa, K. Fujitani, E. Tokunaga, S. Furuya, and K. Inokuchi The microtubule destabilizer stathmin mediates the development of dendritic arbors in neuronal cells J. Cell Sci., April 15, 2007; 120(8): 1447 - 1456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Baldassa, N. Gnesutta, U. Fascio, E. Sturani, and R. Zippel SCLIP, a Microtubule-destabilizing Factor, Interacts with RasGRF1 and Inhibits Its Ability to Promote Rac Activation and Neurite Outgrowth J. Biol. Chem., January 26, 2007; 282(4): 2333 - 2345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Horiuchi, A. Asada, S.-i. Hisanaga, A. Toh-e, and M. Nishizawa Identifying novel substrates for mouse Cdk5 kinase using the yeast Saccharomyces cerevisiae Genes Cells, December 1, 2006; 11(12): 1393 - 1404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tamura, M. Yoshie, H. Nishi, Y. Osakabe, K. Isaka, T. Hara, and H. Kogo Expression of stathmin in human uterus and decidualizing endometrial stromal cells. Reproduction, October 1, 2006; 132(4): 625 - 636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Mense, A. Sengupta, C. Lan, M. Zhou, G. Bentsman, D. J. Volsky, R. M. Whyatt, F. P. Perera, and L. Zhang The Common Insecticides Cyfluthrin and Chlorpyrifos Alter the Expression of a Subset of Genes with Diverse Functions in Primary Human Astrocytes Toxicol. Sci., September 1, 2006; 93(1): 125 - 135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Manna, D. Thrower, H. P. Miller, P. Curmi, and L. Wilson Stathmin Strongly Increases the Minus End Catastrophe Frequency and Induces Rapid Treadmilling of Bovine Brain Microtubules at Steady State in Vitro J. Biol. Chem., January 27, 2006; 281(4): 2071 - 2078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. H. Ng, B. H. Lin, C. P. Lim, G. Huang, T. Zhang, V. Poli, and X. Cao Stat3 regulates microtubules by antagonizing the depolymerization activity of stathmin J. Cell Biol., January 17, 2006; 172(2): 245 - 257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Charbaut, S. Chauvin, H. Enslen, S. Zamaroczy, and A. Sobel Two separate motifs cooperate to target stathmin-related proteins to the Golgi complex J. Cell Sci., May 15, 2005; 118(10): 2313 - 2323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Unwin, D. W. Sternberg, Y. Lu, A. Pierce, D. G. Gilliland, and A. D. Whetton Global Effects of BCR/ABL and TEL/PDGFR{beta} Expression on the Proteome and Phosphoproteome: IDENTIFICATION OF THE RHO PATHWAY AS A TARGET OF BCR/ABL J. Biol. Chem., February 25, 2005; 280(8): 6316 - 6326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Strey, D. Spellman, A. Stieber, J. O. Gonatas, X. Wang, J. D. Lambris, and N. K. Gonatas Dysregulation of Stathmin, a Microtubule-Destabilizing Protein, and Up-Regulation of Hsp25, Hsp27, and the Antioxidant Peroxiredoxin 6 in a Mouse Model of Familial Amyotrophic Lateral Sclerosis Am. J. Pathol., November 1, 2004; 165(5): 1701 - 1718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Lallemand-Breitenbach, M. Quesnoit, V. Braun, A. El Marjou, C. Pous, B. Goud, and F. Perez CLIPR-59 Is a Lipid Raft-associated Protein Containing a Cytoskeleton-associated Protein Glycine-rich Domain (CAP-Gly) That Perturbs Microtubule Dynamics J. Biol. Chem., September 24, 2004; 279(39): 41168 - 41178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Zahedi, Z. Wang, S. Barone, K. Tehrani, N. Yokota, S. Petrovic, H. Rabb, and M. Soleimani Identification of stathmin as a novel marker of cell proliferation in the recovery phase of acute ischemic renal failure Am J Physiol Cell Physiol, May 1, 2004; 286(5): C1203 - C1211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ban, Y. Irino, K. Fukami, and H. Tanaka Human Mitotic Spindle-associated Protein PRC1 Inhibits MgcRacGAP Activity toward Cdc42 during the Metaphase J. Biol. Chem., April 16, 2004; 279(16): 16394 - 16402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wittmann, G. M. Bokoch, and C. M. Waterman-Storer Regulation of Microtubule Destabilizing Activity of Op18/Stathmin Downstream of Rac1 J. Biol. Chem., February 13, 2004; 279(7): 6196 - 6203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Walter-Yohrling, X. Cao, M. Callahan, W. Weber, S. Morgenbesser, S. L. Madden, C. Wang, and B. A. Teicher Identification of Genes Expressed in Malignant Cells That Promote Invasion Cancer Res., December 15, 2003; 63(24): 8939 - 8947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Honnappa, B. Cutting, W. Jahnke, J. Seelig, and M. O. Steinmetz Thermodynamics of the Op18/Stathmin-Tubulin Interaction J. Biol. Chem., October 3, 2003; 278(40): 38926 - 38934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Holmfeldt, K. Brannstrom, S. Stenmark, and M. Gullberg Deciphering the Cellular Functions of the Op18/Stathmin Family of Microtubule-Regulators by Plasma Membrane-targeted Localization Mol. Biol. Cell, September 1, 2003; 14(9): 3716 - 3729. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Chen, H. Wang, T. G. Gharib, C.-C. Huang, D. G. Thomas, K. A. Shedden, R. Kuick, J. M. G. Taylor, S. L. R. Kardia, D. E. Misek, et al. Overexpression of Oncoprotein 18 Correlates with Poor Differentiation in Lung Adenocarcinomas Mol. Cell. Proteomics, February 1, 2003; 2(2): 107 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Alli, J. Bash-Babula, J.-M. Yang, and W. N. Hait Effect of Stathmin on the Sensitivity to Antimicrotubule Drugs in Human Breast Cancer Cancer Res., December 1, 2002; 62(23): 6864 - 6869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu, T. K. Chatterjee, and R. A. Fisher RGS6 Interacts with SCG10 and Promotes Neuronal Differentiation. ROLE OF THE G GAMMA SUBUNIT-LIKE (GGL) DOMAIN OF RGS6 J. Biol. Chem., September 27, 2002; 277(40): 37832 - 37839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Amayed, D. Pantaloni, and M.-F. Carlier The Effect of Stathmin Phosphorylation on Microtubule Assembly Depends on Tubulin Critical Concentration J. Biol. Chem., June 14, 2002; 277(25): 22718 - 22724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Nixon, G. Grenningloh, and P. J. Casey The Interaction of RGSZ1 with SCG10 Attenuates the Ability of SCG10 to Promote Microtubule Disassembly J. Biol. Chem., May 10, 2002; 277(20): 18127 - 18133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. P. Budde, A. Kumagai, W. G. Dunphy, and R. Heald Regulation of Op18 during Spindle Assembly in Xenopus Egg Extracts J. Cell Biol., April 2, 2001; 153(1): 149 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Küntziger, O. Gavet, V. Manceau, A. Sobel, and M. Bornens Stathmin/Op18 Phosphorylation Is Regulated by Microtubule Assembly Mol. Biol. Cell, February 1, 2001; 12(2): 437 - 448. [Abstract] [Full Text]
|
|
|
|
|
|
V. Redeker, S. Lachkar, S. Siavoshian, E. Charbaut, J. Rossier, A. Sobel, and P. A. Curmi Probing the Native Structure of Stathmin and Its Interaction Domains with Tubulin. COMBINED USE OF LIMITED PROTEOLYSIS, SIZE EXCLUSION CHROMATOGRAPHY, AND MASS SPECTROMETRY J. Biol. Chem., March 15, 2000; 275(10): 6841 - 6849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y Gachet, S Tournier, M Lee, A Lazaris-Karatzas, T Poulton, and U. Bommer The growth-related, translationally controlled protein P23 has properties of a tubulin binding protein and associates transiently with microtubules during the cell cycle J. Cell Sci., January 4, 1999; 112(8): 1257 - 1271. [Abstract] [PDF]
|
|
|
|
|
|
H. Daub, K. Gevaert, J. Vandekerckhove, A. Sobel, and A. Hall Rac/Cdc42 and p65PAK Regulate the Microtubule-destabilizing Protein Stathmin through Phosphorylation at Serine 16 J. Biol. Chem., January 12, 2001; 276(3): 1677 - 1680. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Tasken, P. Collas, W. A. Kemmner, O. Witczak, M. Conti, and K. Tasken Phosphodiesterase 4D and Protein Kinase A Type II Constitute a Signaling Unit in the Centrosomal Area J. Biol. Chem., June 15, 2001; 276(25): 21999 - 22002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Charbaut, P. A. Curmi, S. Ozon, S. Lachkar, V. Redeker, and A. Sobel Stathmin Family Proteins Display Specific Molecular and Tubulin Binding Properties J. Biol. Chem., May 4, 2001; 276(19): 16146 - 16154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kuntziger, O. Gavet, A. Sobel, and M. Bornens Differential Effect of Two Stathmin/Op18 Phosphorylation Mutants on Xenopus Embryo Development J. Biol. Chem., June 15, 2001; 276(25): 22979 - 22984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ozon, A. Guichet, O. Gavet, S. Roth, and A. Sobel Drosophila Stathmin: A Microtubule-destabilizing Factor Involved in Nervous System Formation Mol. Biol. Cell, February 1, 2002; 13(2): 698 - 710. [Abstract] [Full Text] [PDF]
|
|
