Journal of Cell Science, Vol 111, Issue 23 3541-3550, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

Expression and potential role of Fsrg1, a murine bromodomain-containing homologue of the Drosophila gene female sterile homeotic

K Rhee, M Brunori, V Besset, R Trousdale and DJ Wolgemuth
Department of Genetics & Development, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.

We have isolated a cDNA which is a murine homologue of the Drosophila gene female sterile homeotic (fsh). This homologue, which we have designated Fsrg1*, contains two bromodomains and an ET motif characteristic of the Fsh sub-class of bromodomain-containing proteins. Northern blot hybridization analysis of adult tissues revealed that Fsrg1 was expressed at low levels rather ubiquitously, but most abundantly in the testis and ovary. Polyclonal antibodies raised against an Fsrg1 fusion protein were used to characterize the Fsrg1 gene product in tissues. Constructs were also generated in which the Fsrg1 cDNA was tagged with epitopes for hemaglutinin and used in transfection experiments. Immunoblot analysis revealed that the Fsrg1 protein migrates with a relative molecular mass of approximately 110 kDa, although the cDNA sequence would predict a protein of approximately 88 kDa. The migration at approximately 110 kDa was observed for both in vivo protein and protein produced in cultured cells. The Fsrg1 protein was localized to the nucleus when expressed in cultured cells, consistent with the presence of a nuclear localization signal motif in the Fsrg1 sequence. No kinase activity was detected for this nuclear protein as assessed in either autokinase or specific substrate assays. In situ hybridization analysis revealed strikingly high expression of Fsrg1 in granulosa cells of growing follicles in the adult ovary and suggested its possible involvement in folliculogenesis. Additional clues to its potential function were provided by the demonstration of its high level of expression in epithelia of tissues which undergo hormonally-modulated remodeling.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				Y.-L. Chang, B. King, S.-C. Lin, J. A. Kennison, and D.-H. Huang A Double-Bromodomain Protein, FSH-S, Activates the Homeotic Gene Ultrabithorax through a Critical Promoter-Proximal Region Mol. Cell. Biol., August 1, 2007; 27(15): 5486 - 5498. [Abstract] [Full Text] [PDF]

				R. M. Zayas, A. Hernandez, B. Habermann, Y. Wang, J. M. Stary, and P. A. Newmark The planarian Schmidtea mediterranea as a model for epigenetic germ cell specification: Analysis of ESTs from the hermaphroditic strain PNAS, December 20, 2005; 102(51): 18491 - 18496. [Abstract] [Full Text] [PDF]

				A. Boyer, J. G. Lussier, A. H. Sinclair, P. J. McClive, and D. W. Silversides Pre-Sertoli Specific Gene Expression Profiling Reveals Differential Expression of Ppt1 and Brd3 Genes Within the Mouse Genital Ridge at the Time of Sex Determination Biol Reprod, September 1, 2004; 71(3): 820 - 827. [Abstract] [Full Text] [PDF]

				A. Dey, F. Chitsaz, A. Abbasi, T. Misteli, and K. Ozato The double bromodomain protein Brd4 binds to acetylated chromatin during interphase and mitosis PNAS, July 22, 2003; 100(15): 8758 - 8763. [Abstract] [Full Text] [PDF]

				T. Maruyama, A. Farina, A. Dey, J. Cheong, V. P. Bermudez, T. Tamura, S. Sciortino, J. Shuman, J. Hurwitz, and K. Ozato A Mammalian Bromodomain Protein, Brd4, Interacts with Replication Factor C and Inhibits Progression to S Phase Mol. Cell. Biol., September 15, 2002; 22(18): 6509 - 6520. [Abstract] [Full Text] [PDF]

				T. E. Crowley, E. M. Kaine, M. Yoshida, A. Nandi, and D. J. Wolgemuth Reproductive Cycle Regulation of Nuclear Import, Euchromatic Localization, and Association with Components of Pol II Mediator of a Mammalian Double-Bromodomain Protein Mol. Endocrinol., August 1, 2002; 16(8): 1727 - 1737. [Abstract] [Full Text] [PDF]

				D. Houzelstein, S. L. Bullock, D. E. Lynch, E. F. Grigorieva, V. A. Wilson, and R. S. P. Beddington Growth and Early Postimplantation Defects in Mice Deficient for the Bromodomain-Containing Protein Brd4{dagger} Mol. Cell. Biol., June 1, 2002; 22(11): 3794 - 3802. [Abstract] [Full Text] [PDF]

				K. Mattsson, C. Kiss, G. M. Platt, G. R. Simpson, E. Kashuba, G. Klein, T. F. Schulz, and L. Szekely Latent nuclear antigen of Kaposi's sarcoma herpesvirus/human herpesvirus-8 induces and relocates RING3 to nuclear heterochromatin regions J. Gen. Virol., January 1, 2002; 83(1): 179 - 188. [Abstract] [Full Text] [PDF]

				D. R. Schwam, R. L. Luciano, S. S. Mahajan, L. Wong, and A. C. Wilson Carboxy Terminus of Human Herpesvirus 8 Latency-Associated Nuclear Antigen Mediates Dimerization, Transcriptional Repression, and Targeting to Nuclear Bodies J. Virol., September 15, 2000; 74(18): 8532 - 8540. [Abstract] [Full Text]

				A. Dey, J. Ellenberg, A. Farina, A. E. Coleman, T. Maruyama, S. Sciortino, J. Lippincott-Schwartz, and K. Ozato A Bromodomain Protein, MCAP, Associates with Mitotic Chromosomes and Affects G2-to-M Transition Mol. Cell. Biol., September 1, 2000; 20(17): 6537 - 6549. [Abstract] [Full Text]

				G. M. Platt, G. R. Simpson, S. Mittnacht, and T. F. Schulz Latent Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus Interacts with RING3, a Homolog of the Drosophila Female Sterile Homeotic (fsh) Gene J. Virol., December 1, 1999; 73(12): 9789 - 9795. [Abstract] [Full Text]