Journal of Cell Science, Vol 111, Issue 24 3621-3631, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

Distinct signal transduction pathways are utilized during the tube formation and survival phases of in vitro angiogenesis

N Ilan, S Mahooti and JA Madri
Department of Pathology, Yale University School of Medicine, New Haven CT 06510, USA.

Angiogenesis, the formation of new blood vessels from pre-existing ones, occurs during development, wound healing and cancer and involves stages that orchestrate a network of cooperative interactions. Peptide growth factors and extracellular matrix (ECM) components are two major groups of angiogenesis mediators. Among the different ECM proteins, collagens have been well-associated with in vivo angiogenesis. Using human umbilical vein endothelial cells (HUVEC) grown in 3-D collagen gels we show that: (1) HUVEC do not survive well in 3-D collagen gels due to rapid induction of apoptosis. (2) VEGF, a potent in vivo angiogenic factor, fails to induce tube formation. (3) PMA was effective in inducing tube formation and survival in HUVEC dispersed in 3-D collagen gels, activating MAP kinase, phosphoinositide 3-OH kinase (PI-3-kinase) and Akt/PKB (protein kinase B) pathways. (4) VEGF was effective in preventing PMA-induced tube-like structure regression after PMA-withdrawal by (5) activating the mitogen activated protein kinase (MAPK), rather than the Akt/PKB, signaling pathway.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. Tapon-Bretaudiere, D. Chabut, M. Zierer, S. Matou, D. Helley, A. Bros, P. A.S. Mourao, and A.-M. Fischer A Fucosylated Chondroitin Sulfate From Echinoderm Modulates in Vitro Fibroblast Growth Factor 2-Dependent Angiogenesis Mol. Cancer Res., December 1, 2002; 1(2): 96 - 102. [Abstract] [Full Text] [PDF]

				M. Zygmunt, F. Herr, S. Keller-Schoenwetter, K. Kunzi-Rapp, K. Munstedt, C. V. Rao, U. Lang, and K. T. Preissner Characterization of Human Chorionic Gonadotropin as a Novel Angiogenic Factor J. Clin. Endocrinol. Metab., November 1, 2002; 87(11): 5290 - 5296. [Abstract] [Full Text] [PDF]

				M. E. Gerritsen, R. Soriano, S. Yang, G. Ingle, C. Zlot, K. Toy, J. Winer, A. Draksharapu, F. Peale, T. D. Wu, et al. In silico data filtering to identify new angiogenesis targets from a large in vitro gene profiling data set Physiol Genomics, July 12, 2002; 10(1): 13 - 20. [Abstract] [Full Text] [PDF]

				M. A. Lafleur, M. M. Handsley, V. Knauper, G. Murphy, and D. R. Edwards Endothelial tubulogenesis within fibrin gels specifically requires the activity of membrane-type-matrix metalloproteinases (MT-MMPs) J. Cell Sci., January 9, 2002; 115(17): 3427 - 3438. [Abstract] [Full Text] [PDF]

				B. A. Ruggeri, C. Robinson, T. Angeles, J. Wilkinson IV, and M. L. Clapper The Chemopreventive Agent Oltipraz Possesses Potent Antiangiogenic Activity in Vitro, ex Vivo, and in Vivo and Inhibits Tumor Xenograft Growth Clin. Cancer Res., January 1, 2002; 8(1): 267 - 274. [Abstract] [Full Text] [PDF]

				F. Vinals and J. Pouyssegur Transforming Growth Factor {beta}1 (TGF-{beta}1) Promotes Endothelial Cell Survival during In Vitro Angiogenesis via an Autocrine Mechanism Implicating TGF-{alpha} Signaling Mol. Cell. Biol., November 1, 2001; 21(21): 7218 - 7230. [Abstract] [Full Text] [PDF]

				C. C. Solorzano, Y. D. Jung, C. D. Bucana, D. J. McConkey, G. E. Gallick, G. McMahon, and L. M. Ellis In Vivo Intracellular Signaling as a Marker of Antiangiogenic Activity Cancer Res., October 1, 2001; 61(19): 7048 - 7051. [Abstract] [Full Text] [PDF]

				Z. Wang, C. Jiang, H. Ganther, and J. Lu Antimitogenic and Proapoptotic Activities of Methylseleninic Acid in Vascular Endothelial Cells and Associated Effects on PI3K-AKT, ERK, JNK and p38 MAPK Signaling Cancer Res., October 1, 2001; 61(19): 7171 - 7178. [Abstract] [Full Text] [PDF]

				M. Mesri, M. Morales-Ruiz, E. J. Ackermann, C. F. Bennett, J. S. Pober, W. C. Sessa, and D. C. Altieri Suppression of Vascular Endothelial Growth Factor-Mediated Endothelial Cell Protection by Survivin Targeting Am. J. Pathol., May 1, 2001; 158(5): 1757 - 1765. [Abstract] [Full Text] [PDF]

				M. Strasly, F. Cavallo, M. Geuna, S. Mitola, M. P. Colombo, G. Forni, and F. Bussolino IL-12 Inhibition of Endothelial Cell Functions and Angiogenesis Depends on Lymphocyte-Endothelial Cell Cross-Talk J. Immunol., March 15, 2001; 166(6): 3890 - 3899. [Abstract] [Full Text] [PDF]

				X. Xin, S. Yang, G. Ingle, C. Zlot, L. Rangell, J. Kowalski, R. Schwall, N. Ferrara, and M. E. Gerritsen Hepatocyte Growth Factor Enhances Vascular Endothelial Growth Factor-Induced Angiogenesis in Vitro and in Vivo Am. J. Pathol., March 1, 2001; 158(3): 1111 - 1120. [Abstract] [Full Text] [PDF]

				N. ILAN, A. MOHSENIN, L. CHEUNG, and J. A. MADRI PECAM-1 shedding during apoptosis generates a membrane-anchored truncated molecule with unique signaling characteristics FASEB J, February 1, 2001; 15(2): 362 - 372. [Abstract] [Full Text] [PDF]

				S. E. Bell, A. Mavila, R. Salazar, K. J. Bayless, S. Kanagala, S. A. Maxwell, and G. E. Davis Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling J. Cell Sci., January 8, 2001; 114(15): 2755 - 2773. [Abstract] [Full Text] [PDF]

				S. Kim, K. Bell, S. A. Mousa, and J. A. Varner Regulation of Angiogenesis in Vivo by Ligation of Integrin {alpha}5{beta}1 with the Central Cell-Binding Domain of Fibronectin Am. J. Pathol., April 1, 2000; 156(4): 1345 - 1362. [Abstract] [Full Text] [PDF]

				H Paradis and R. Gendron LIF transduces contradictory signals on capillary outgrowth through induction of stat3 and (P41/43)MAP kinase J. Cell Sci., January 12, 2000; 113(23): 4331 - 4339. [Abstract] [PDF]

				A. J. Singer and R. A.F. Clark Cutaneous Wound Healing N. Engl. J. Med., September 2, 1999; 341(10): 738 - 746. [Full Text] [PDF]

				S. Yang, J. Graham, J. W. Kahn, E. A. Schwartz, and M. E. Gerritsen Functional Roles for PECAM-1 (CD31) and VE-Cadherin (CD144) in Tube Assembly and Lumen Formation in Three-Dimensional Collagen Gels Am. J. Pathol., September 1, 1999; 155(3): 887 - 895. [Abstract] [Full Text] [PDF]

				X. Xin, S. Yang, J. Kowalski, and M. E. Gerritsen Peroxisome Proliferator-activated Receptor gamma  Ligands Are Potent Inhibitors of Angiogenesis in Vitro and in Vivo J. Biol. Chem., March 26, 1999; 274(13): 9116 - 9121. [Abstract] [Full Text] [PDF]

				N Ilan, S Mahooti, D. Rimm, and J. Madri PECAM-1 (CD31) functions as a reservoir for and a modulator of tyrosine-phosphorylated beta-catenin J. Cell Sci., January 9, 1999; 112(18): 3005 - 3014. [Abstract] [PDF]

				R Giuliani, M Bastaki, D Coltrini, and M Presta Role of endothelial cell extracellular signal-regulated kinase1/2 in urokinase-type plasminogen activator upregulation and in vitro angiogenesis by fibroblast growth factor-2 J. Cell Sci., January 8, 1999; 112(15): 2597 - 2606. [Abstract] [PDF]

				N. Ilan, L. Cheung, E. Pinter, and J. A. Madri Platelet-Endothelial Cell Adhesion Molecule-1 (CD31), a Scaffolding Molecule for Selected Catenin Family Members Whose Binding Is Mediated by Different Tyrosine and Serine/Threonine Phosphorylation J. Biol. Chem., July 7, 2000; 275(28): 21435 - 21443. [Abstract] [Full Text] [PDF]

				J. S. Schechner, A. K. Nath, L. Zheng, M. S. Kluger, C. C. W. Hughes, M. R. Sierra-Honigmann, M. I. Lorber, G. Tellides, M. Kashgarian, A. L. M. Bothwell, et al. In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse PNAS, August 1, 2000; 97(16): 9191 - 9196. [Abstract] [Full Text] [PDF]

				L. Vincent, C. Soria, F. Mirshahi, P. Opolon, Z. Mishal, J.-P. Vannier, J. Soria, and L. Hong Cerivastatin, an Inhibitor of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase, Inhibits Endothelial Cell Proliferation Induced by Angiogenic Factors In Vitro and Angiogenesis in In Vivo Models Arterioscler Thromb Vasc Biol, April 1, 2002; 22(4): 623 - 629. [Abstract] [Full Text] [PDF]