|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 3 313-320, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
KA Johnson
Department of Biology, Haverford College, Haverford, PA 19041, USA. kjohnson@haverford.edu
Little is known of the molecular basis for the diversity of microtubule structure and function found within the eukaryotic flagellum. Antibodies that discriminate between tyrosinated alpha tubulin and post-translationally detyrosinated alpha tubulin were used to localize these complementary tubulin isoforms in flagella of the single-celled green alga Chlamydomonas reinhardtii. Immunofluorescence analysis of intact axonemes detected both isoforms along most of the lengths of flagella; however, each had a short distal zone rich in tyrosinated tubulin. Localizations on splayed axonemes revealed that the microtubules of the central-pair apparatus were rich in tyrosinated tubulin, while outer doublets contained a mixture of both isoforms. Immunoelectron analysis of individual outer doublets revealed that while tyrosinated tubulin was present in both A and B tubules, detyrosinated tubulin was largely confined to the wall of the B hemi-tubules. The absence of detyrosinated tubulin from the A tubules of the outer doublets and the microtubules of the central pair, both of which extend past the B hemi-tubules of the outer doublets in the flagellar tip, explained the appearance of a tyrosinated tubulin-rich distal zone on intact axonemes. Localizations performed on cells regenerating flagella revealed that flagellar assembly used tyrosinated tubulin; detyrosination of the B tubule occurred during later stages of regeneration, well after microtubule polymerization. The developmental timing of detyrosination, which occurs over a period during which the regrowing flagella begin to beat more effectively, suggests that post-translational modification of the B tubule surface may enhance dynein/B tubule interactions that power flagellar beating.
This article has been cited by other articles:
|
|
 |
|
  A. Wienecke and G. Bacher Indibulin, a Novel Microtubule Inhibitor, Discriminates between Mature Neuronal and Nonneuronal Tubulin Cancer Res., January 1, 2009; 69(1): 171 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Shapiro, J. Ingram, and K. A. Johnson Characterization of a Molecular Chaperone Present in the Eukaryotic Flagellum Eukaryot. Cell, September 1, 2005; 4(9): 1591 - 1594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Thazhath, M. Jerka-Dziadosz, J. Duan, D. Wloga, M. A. Gorovsky, J. Frankel, and J. Gaertig Cell Context-specific Effects of the {beta}-Tubulin Glycylation Domain on Assembly and Size of Microtubular Organelles Mol. Biol. Cell, September 1, 2004; 15(9): 4136 - 4147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. F. Marshall and J. L. Rosenbaum Intraflagellar transport balances continuous turnover of outer doublet microtubules: implications for flagellar length control J. Cell Biol., October 29, 2001; 155(3): 405 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Sapiro, L. M. Tarantino, F. Velazquez, M. Kiriakidou, N. B. Hecht, M. Bucan, and J. F. Strauss III Sperm Antigen 6 Is the Murine Homologue of the Chlamydomonas reinhardtii Central Apparatus Protein Encoded by the PF16 Locus Biol Reprod, March 1, 2000; 62(3): 511 - 518. [Abstract] [Full Text]
|
|
