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Journal of Cell Science, Vol 111, Issue 3 405-412, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
U Euteneuer, R Graf, E Kube-Granderath and M Schliwa
Adolf-Butenandt-Institut, Zellbiologie, Ludwig-Maximilians-Universitat Munchen, Germany. ursula.euteneuer@lrz.uni-muenchen.de
The centrosome of Dictyostelium discoideum is a nucleus-associated body consisting of an electron-dense, three-layered core surrounded by an amorphous matrix, the corona. To elucidate the molecular and supramolecular architecture of this unique microtubule-organizing center, we have isolated and sequenced the gene encoding gamma-tubulin and have studied its localization in the Dictyostelium centrosome using immunofluorescence and postembedding immunoelectron microscopy. D. discoideum possesses a single copy of a gamma-tubulin gene that is related to, but more divergent from, other gamma-tubulins. The low-abundance gene product is localized to the centrosome in an intriguing pattern: it is highly concentrated in the corona in regularly spaced clusters whose distribution correlates with the patterning of dense nodules that are a prominent feature of the corona. These observations lend support to the notion that the corona is the functional homologue of the pericentriolar matrix of 'higher' eukaryotic centrosomes, and that nodules are the functional equivalent of gamma-tubulin ring complexes that serve as nucleation sites for microtubules in animal centrosomes.
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  M. Rehberg, J. Kleylein-Sohn, J. Faix, T.-H. Ho, I. Schulz, and R. Graf Dictyostelium LIS1 Is a Centrosomal Protein Required for Microtubule/Cell Cortex Interactions, Nucleus/Centrosome Linkage, and Actin Dynamics Mol. Biol. Cell, June 1, 2005; 16(6): 2759 - 2771. [Abstract] [Full Text] [PDF]
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R. Graf, U. Euteneuer, T.-H. Ho, and M. Rehberg Regulated Expression of the Centrosomal Protein DdCP224 Affects Microtubule Dynamics and Reveals Mechanisms for the Control of Supernumerary Centrosome Number Mol. Biol. Cell, October 1, 2003; 14(10): 4067 - 4074. [Abstract] [Full Text] [PDF]
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 R. Graf DdNek2, the first non-vertebrate homologue of human Nek2, is involved in the formation of microtubule-organizing centers J. Cell Sci., January 5, 2002; 115(9): 1919 - 1929. [Abstract] [Full Text] [PDF]
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R Graf, C Daunderer, and M Schliwa Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication J. Cell Sci., January 5, 2000; 113(10): 1747 - 1758. [Abstract] [PDF]
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 S. Ma, L. Trivinos-Lagos, R. Graf, and R. L. Chisholm Dynein Intermediate Chain Mediated Dynein-Dynactin Interaction Is Required for Interphase Microtubule Organization and Centrosome Replication and Separation in Dictyostelium J. Cell Biol., December 13, 1999; 147(6): 1261 - 1274. [Abstract] [Full Text] [PDF]
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M. Ueda, M. Schliwa, and U. Euteneuer Unusual Centrosome Cycle in Dictyostelium: Correlation of Dynamic Behavior and Structural Changes Mol. Biol. Cell, January 1, 1999; 10(1): 151 - 160. [Abstract] [Full Text]
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