spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Euteneuer, U.
Right arrow Articles by Schliwa, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Euteneuer, U.
Right arrow Articles by Schliwa, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 111, Issue 3 405-412, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

Dictyostelium gamma-tubulin: molecular characterization and ultrastructural localization

U Euteneuer, R Graf, E Kube-Granderath and M Schliwa
Adolf-Butenandt-Institut, Zellbiologie, Ludwig-Maximilians-Universitat Munchen, Germany. ursula.euteneuer@lrz.uni-muenchen.de

The centrosome of Dictyostelium discoideum is a nucleus-associated body consisting of an electron-dense, three-layered core surrounded by an amorphous matrix, the corona. To elucidate the molecular and supramolecular architecture of this unique microtubule-organizing center, we have isolated and sequenced the gene encoding gamma-tubulin and have studied its localization in the Dictyostelium centrosome using immunofluorescence and postembedding immunoelectron microscopy. D. discoideum possesses a single copy of a gamma-tubulin gene that is related to, but more divergent from, other gamma-tubulins. The low-abundance gene product is localized to the centrosome in an intriguing pattern: it is highly concentrated in the corona in regularly spaced clusters whose distribution correlates with the patterning of dense nodules that are a prominent feature of the corona. These observations lend support to the notion that the corona is the functional homologue of the pericentriolar matrix of 'higher' eukaryotic centrosomes, and that nodules are the functional equivalent of gamma-tubulin ring complexes that serve as nucleation sites for microtubules in animal centrosomes.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
R. Blau-Wasser, U. Euteneuer, H. Xiong, B. Gassen, M. Schleicher, and A. A. Noegel
CP250, a Novel Acidic Coiled Coil Protein of the Dictyostelium centrosome, Affects Growth, Chemotaxis, and the Nuclear Envelope
Mol. Biol. Cell, October 15, 2009; 20(20): 4348 - 4361.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. Mana-Capelli, R. Graf, and D. A. Larochelle
Dictyostelium discoideum CenB Is a Bona Fide Centrin Essential for Nuclear Architecture and Centrosome Stability
Eukaryot. Cell, August 1, 2009; 8(8): 1106 - 1117.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. Azimzadeh, P. Hergert, A. Delouvee, U. Euteneuer, E. Formstecher, A. Khodjakov, and M. Bornens
hPOC5 is a centrin-binding protein required for assembly of full-length centrioles
J. Cell Biol., April 6, 2009; 185(1): 101 - 114.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Rehberg, J. Kleylein-Sohn, J. Faix, T.-H. Ho, I. Schulz, and R. Graf
Dictyostelium LIS1 Is a Centrosomal Protein Required for Microtubule/Cell Cortex Interactions, Nucleus/Centrosome Linkage, and Actin Dynamics
Mol. Biol. Cell, June 1, 2005; 16(6): 2759 - 2771.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Graf, U. Euteneuer, T.-H. Ho, and M. Rehberg
Regulated Expression of the Centrosomal Protein DdCP224 Affects Microtubule Dynamics and Reveals Mechanisms for the Control of Supernumerary Centrosome Number
Mol. Biol. Cell, October 1, 2003; 14(10): 4067 - 4074.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Graf
DdNek2, the first non-vertebrate homologue of human Nek2, is involved in the formation of microtubule-organizing centers
J. Cell Sci., January 5, 2002; 115(9): 1919 - 1929.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R Graf, C Daunderer, and M Schliwa
Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication
J. Cell Sci., January 5, 2000; 113(10): 1747 - 1758.
[Abstract] [PDF]

Home page
 JCBHome page
S. Ma, L. Trivinos-Lagos, R. Graf, and R. L. Chisholm
Dynein Intermediate Chain Mediated Dynein-Dynactin Interaction Is Required for Interphase Microtubule Organization and Centrosome Replication and Separation inDictyostelium
J. Cell Biol., December 13, 1999; 147(6): 1261 - 1274.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Ueda, M. Schliwa, and U. Euteneuer
Unusual Centrosome Cycle in Dictyostelium: Correlation of Dynamic Behavior and Structural Changes
Mol. Biol. Cell, January 1, 1999; 10(1): 151 - 160.
[Abstract] [Full Text]


© The Company of Biologists Ltd 1998