|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 4 495-509, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
C Marcozzi, ID Burdett, RS Buxton and AI Magee
Division of Membrane Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
Desmosomes are unique intercellular junctions in that they invariably contain two types of transmembrane cadherin molecule, desmocollins and desmogleins. In addition they possess a distinct cytoplasmic plaque structure containing a few major proteins including desmoplakins and the armadillo family member plakoglobin. Desmosomal cadherins are putative cell-cell adhesion molecules and we have tested their adhesive capacity using a transfection approach in mouse L cells. We find that L cells expressing either one or both of the desmosomal cadherins desmocollin 2a or desmoglein 1 display weak cell-cell adhesion activity that is Ca2+-dependent. Both homophilic and heterophilic adhesion could be detected. However, co-expression of plakoglobin with both desmosomal cadherins, but not with desmoglein 1 alone, resulted in a dramatic potentiation of cell-cell aggregation and the accumulation of detergent-insoluble desmosomal proteins at points of cell-cell contact. The effect of plakoglobin seems to be due directly to its interaction with the desmosomal cadherins rather than to its signalling function. The data suggest that the desmosome may obligatorily contain two cadherins and is consistent with a model in which desmocollins and desmogleins may form side by side heterodimers in contrast to the classical cadherins that are homodimeric. Plakoglobin may function by potentiating dimer formation, accretion of dimers to cell-cell contact sites or desmosomal cadherin stability.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  V. Spindler, W.-M. Heupel, A. Efthymiadis, E. Schmidt, R. Eming, C. Rankl, P. Hinterdorfer, T. Muller, D. Drenckhahn, and J. Waschke Desmocollin 3-mediated Binding Is Crucial for Keratinocyte Cohesion and Is Impaired in Pemphigus J. Biol. Chem., October 30, 2009; 284(44): 30556 - 30564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. W. Franke Discovering the Molecular Components of Intercellular Junctions--A Historical View Cold Spring Harb Perspect Biol, September 1, 2009; 1(3): a003061 - a003061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Delva, D. K. Tucker, and A. P. Kowalczyk The Desmosome Cold Spring Harb Perspect Biol, August 1, 2009; 1(2): a002543 - a002543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Streets, B. E. Wagner, P. C. Harris, C. J. Ward, and A. C. M. Ong Homophilic and heterophilic polycystin 1 interactions regulate E-cadherin recruitment and junction assembly in MDCK cells J. Cell Sci., May 1, 2009; 122(9): 1410 - 1417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-M. Heupel, D. Zillikens, D. Drenckhahn, and J. Waschke Pemphigus Vulgaris IgG Directly Inhibit Desmoglein 3-Mediated Transinteraction J. Immunol., August 1, 2008; 181(3): 1825 - 1834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Mruk, B. Silvestrini, and C. Y. Cheng Anchoring Junctions As Drug Targets: Role in Contraceptive Development Pharmacol. Rev., June 1, 2008; 60(2): 146 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Chandramouli, J. Shi, Y. Feng, H. Holubec, R. M.Shanas, A. K. Bhattacharyya, W. Zheng, and M. A. Nelson Haploinsufficiency of the cdc2l gene contributes to skin cancer development in mice Carcinogenesis, September 1, 2007; 28(9): 2028 - 2035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Cheng, K. Mihindukulasuriya, Z. Den, A. P. Kowalczyk, C. C. Calkins, A. Ishiko, A. Shimizu, and P. J. Koch Assessment of Splice Variant-Specific Functions of Desmocollin 1 in the Skin Mol. Cell. Biol., January 1, 2004; 24(1): 154 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ortiz-Urda, A. Elbe-Burger, J. Smolle, Y. Marquart, Y. Chudnovsky, T. W. Ridky, P. Bernstein, K. Wolff, and K. Rappersberger The Plant Lectin Wheat Germ Agglutinin Inhibits the Binding of Pemphigus Foliaceus Autoantibodies to Desmoglein 1 in a Majority of Patients and Prevents Pathomechanisms of Pemphigus Foliaceus In Vitro and In Vivo J. Immunol., December 1, 2003; 171(11): 6244 - 6250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Davis, M. K. Duncan, W. G. Robison Jr, and J. Piatigorsky Requirement for Pax6 in corneal morphogenesis: a role in adhesion J. Cell Sci., June 1, 2003; 116(11): 2157 - 2167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Angst, C Marcozzi, and A. Magee The cadherin superfamily: diversity in form and function J. Cell Sci., January 2, 2001; 114(4): 629 - 641. [Abstract] [PDF]
|
|
|
|
|
|
N. Chitaev, A. Averbakh, R. Troyanovsky, and S. Troyanovsky Molecular organization of the desmoglein-plakoglobin complex J. Cell Sci., June 8, 2000; 111(14): 1941 - 1949. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Wallis, S. Lloyd, I. Wise, G. Ireland, T. P. Fleming, and D. Garrod The alpha Isoform of Protein Kinase C Is Involved in Signaling the Response of Desmosomes to Wounding in Cultured Epithelial Cells Mol. Biol. Cell, March 1, 2000; 11(3): 1077 - 1092. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. J. de Boer, E. van Dorst, H. van Krieken, C. M. Jansen-van Rhijn, S. O. Warnaar, G. J. Fleuren, and S. V. Litvinov Changing Roles of Cadherins and Catenins during Progression of Squamous Intraepithelial Lesions in the Uterine Cervix Am. J. Pathol., August 1, 1999; 155(2): 505 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Bierkamp, H Schwarz, O Huber, and R Kemler Desmosomal localization of beta-catenin in the skin of plakoglobin null-mutant mice Development, January 1, 1999; 126(2): 371 - 381. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Tselepis, M. Chidgey, A. North, and D. Garrod Desmosomal adhesion inhibits invasive behavior PNAS, July 7, 1998; 95(14): 8064 - 8069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Norvell and K. Green Contributions of extracellular and intracellular domains of full length and chimeric cadherin molecules to junction assembly in epithelial cells J. Cell Sci., January 5, 1998; 111(9): 1305 - 1318. [Abstract] [PDF]
|
|
|
|
|
|
J. Weiske, T. Schoneberg, W. Schroder, M. Hatzfeld, R. Tauber, and O. Huber The Fate of Desmosomal Proteins in Apoptotic Cells J. Biol. Chem., October 26, 2001; 276(44): 41175 - 41181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Chidgey, C. Brakebusch, E. Gustafsson, A. Cruchley, C. Hail, S. Kirk, A. Merritt, A. North, C. Tselepis, J. Hewitt, et al. Mice lacking desmocollin 1 show epidermal fragility accompanied by barrier defects and abnormal differentiation J. Cell Biol., November 26, 2001; 155(5): 821 - 832. [Abstract] [Full Text] [PDF]
|
|
