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Journal of Cell Science, Vol 111, Issue 5 557-572, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
C Roghi, R Giet, R Uzbekov, N Morin, I Chartrain, R Le Guellec, A Couturier, M Doree, M Philippe and C Prigent
Departement de Biologie et Genetique du Developpement, Universite de Rennes I, Rennes, France.
By differential screening of a Xenopus laevis egg cDNA library, we have isolated a 2,111 bp cDNA which corresponds to a maternal mRNA specifically deadenylated after fertilisation. This cDNA, called Eg2, encodes a 407 amino acid protein kinase. The pEg2 sequence shows significant identity with members of a new protein kinase sub-family which includes Aurora from Drosophila and Ipl1 (increase in ploidy-1) from budding yeast, enzymes involved in centrosome migration and chromosome segregation, respectively. A single 46 kDa polypeptide, which corresponds to the deduced molecular mass of pEg2, is immunodetected in Xenopus oocyte and egg extracts, as well as in lysates of Xenopus XL2 cultured cells. In XL2 cells, pEg2 is immunodetected only in S, G2 and M phases of the cell cycle, where it always localises to the centrosomal region of the cell. In addition, pEg2 'invades' the microtubules at the poles of the mitotic spindle in metaphase and anaphase. Immunoelectron microscopy experiments show that pEg2 is located precisely around the pericentriolar material in prophase and on the spindle microtubules in anaphase. We also demonstrate that pEg2 binds directly to taxol stabilised microtubules in vitro. In addition, we show that the presence of microtubules during mitosis is not necessary for an association between pEg2 and the centrosome. Finally we show that a catalytically inactive pEg2 kinase stops the assembly of bipolar mitotic spindles in Xenopus egg extracts.
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D. C. Farruggio, F. M. Townsley, and J. V. Ruderman Cdc20 associates with the kinase aurora2/Aik PNAS, June 22, 1999; 96(13): 7306 - 7311. [Abstract] [Full Text] [PDF]
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R. Giet, R. Uzbekov, F. Cubizolles, K. Le Guellec, and C. Prigent The Xenopus laevis Aurora-related Protein Kinase pEg2 Associates with and Phosphorylates the Kinesin-related Protein XlEg5 J. Biol. Chem., May 21, 1999; 274(21): 15005 - 15013. [Abstract] [Full Text] [PDF]
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M. Kimura, Y. Matsuda, T. Yoshioka, and Y. Okano Cell Cycle-dependent Expression and Centrosome Localization of a Third Human Aurora/Ipl1-related Protein Kinase, AIK3 J. Biol. Chem., March 12, 1999; 274(11): 7334 - 7340. [Abstract] [Full Text] [PDF]
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R Giet and C Prigent Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases J. Cell Sci., January 11, 1999; 112(21): 3591 - 3601. [Abstract] [PDF]
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J. M. Schumacher, A. Golden, and P. J. Donovan AIR-2: An Aurora/Ipl1-related Protein Kinase Associated with Chromosomes and Midbody Microtubules Is Required for Polar Body Extrusion and Cytokinesis in Caenorhabditis elegans Embryos J. Cell Biol., December 14, 1998; 143(6): 1635 - 1646. [Abstract] [Full Text] [PDF]
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P. Gowdy, H. Anderson, and M Roberge Entry into mitosis without Cdc2 kinase activation J. Cell Sci., January 11, 1998; 111(22): 3401 - 3410. [Abstract] [PDF]
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I. Hagan The fission yeast microtubule cytoskeleton J. Cell Sci., January 6, 1998; 111(12): 1603 - 1612. [Abstract] [PDF]
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R. Giet, D. McLean, S. Descamps, M. J. Lee, J. W. Raff, C. Prigent, and D. M. Glover Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules J. Cell Biol., February 4, 2002; 156(3): 437 - 451. [Abstract] [Full Text] [PDF]
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