|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 5 615-624, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
H Xie, MA Pallero, K Gupta, P Chang, MF Ware, W Witke, DJ Kwiatkowski, DA Lauffenburger, JE Murphy-Ullrich and A Wells
Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama 35294-0007, USA.
A current model of growth factor-induced cell motility invokes integration of diverse biophysical processes required for cell motility, including dynamic formation and disruption of cell/substratum attachments along with extension of membrane protrusions. To define how these biophysical events are actuated by biochemical signaling pathways, we investigate here whether epidermal growth factor (EGF) induces disruption of focal adhesions in fibroblasts. We find that EGF treatment of NR6 fibroblasts presenting full-length WT EGF receptors (EGFR) reduces the fraction of cells presenting focal adhesions from approximately 60% to approximately 30% within 10 minutes. The dose dependency of focal adhesion disassembly mirrors that for EGF-enhanced cell motility, being noted at 0.1 nM EGF. EGFR kinase activity is required as cells expressing two kinase-defective EGFR constructs retain their focal adhesions in the presence of EGF. The short-term (30 minutes) disassembly of focal adhesions is reflected in decreased adhesiveness of EGF-treated cells to substratum. We further examine here known motility-associated pathways to determine whether these contribute to EGF-induced effects. We have previously demonstrated that phospholipase C(gamma) (PLCgamma) activation and mobilization of gelsolin from a plasma membrane-bound state are required for EGFR-mediated cell motility. In contrast, we find here that short-term focal adhesion disassembly is induced by a signaling-restricted truncated EGFR (c'973) which fails to activate PLCgamma or mobilize gelsolin. The PLC inhibitor U73122 has no effect on this process, nor is the actin severing capacity of gelsolin required as EGF treatment reduces focal adhesions in gelsolin-devoid fibroblasts, further supporting the contention that focal adhesion disassembly is signaled by a pathway distinct from that involving PLCgamma. Because both WT and c'973 EGFR activate the erk MAP kinase pathway, we additionally explore here this signaling pathway, not previously associated with growth factor-induced cell motility. Levels of the MEK inhibitor PD98059 that block EGF-induced mitogenesis and MAP kinase phosphorylation also abrogate EGF-induced focal adhesion disassembly and cell motility. In summary, we characterize for the first time the ability of EGFR kinase activity to directly stimulate focal adhesion disassembly and cell/substratum detachment, in relation to its ability to stimulate migration. Furthermore, we propose a model of EGF-induced motogenic cell responses in which the PLCgamma pathway stimulating cell motility is distinct from the MAP kinase-dependent signaling pathway leading to disassembly and reorganization of cell-substratum adhesion.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  I. C. Schneider, C. K. Hays, and C. M. Waterman Epidermal Growth Factor-induced Contraction Regulates Paxillin Phosphorylation to Temporally Separate Traction Generation from De-adhesion Mol. Biol. Cell, July 1, 2009; 20(13): 3155 - 3167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Fiori, T.-N. Zhu, M. P. O'Connell, K. S. Hoek, F. E. Indig, B. P. Frank, C. Morris, S. Kole, J. Hasskamp, G. Elias, et al. Filamin A Modulates Kinase Activation and Intracellular Trafficking of Epidermal Growth Factor Receptors in Human Melanoma Cells Endocrinology, June 1, 2009; 150(6): 2551 - 2560. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Liu, P. Garg, S. Yang, P. Gong, M. A. Pallero, D. S. Annis, Y. Liu, A. Passaniti, D. Mann, D. F. Mosher, et al. Epidermal Growth Factor-like Repeats of Thrombospondins Activate Phospholipase C{gamma} and Increase Epithelial Cell Migration through Indirect Epidermal Growth Factor Receptor Activation J. Biol. Chem., March 6, 2009; 284(10): 6389 - 6402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Ma, D. Zhang, H. Yang, H. Sun, W. Wu, Y. Gan, J. Balducci, Y.-q. Wei, X. Zhao, and Y. Huang Endothelial cell-specific molecule 2 (ECSM2) modulates actin remodeling and epidermal growth factor receptor signaling. Genes Cells, March 1, 2009; 14(3): 281 - 293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Paccione, H. Miyazaki, V. Patel, A. Waseem, J. S. Gutkind, Z. E. Zehner, and W. A. Yeudall Keratin down-regulation in vimentin-positive cancer cells is reversible by vimentin RNA interference, which inhibits growth and motility Mol. Cancer Ther., September 1, 2008; 7(9): 2894 - 2903. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. T. N. Trinh, A. Prive, L. Kheir, J.-C. Bourret, T. Hijazi, M. G. Amraei, J. Noel, and E. Brochiero Involvement of KATP and KvLQT1 K+ channels in EGF-stimulated alveolar epithelial cell repair processes Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L870 - L882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. T. Blikslager, A. J. Moeser, J. L. Gookin, S. L. Jones, and J. Odle Restoration of Barrier Function in Injured Intestinal Mucosa Physiol Rev, April 1, 2007; 87(2): 545 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. Rao, L. Liu, T. Zou, B. S. Marasa, D. Boneva, S. R. Wang, D. L. Malone, D. J. Turner, and J.-Y. Wang Polyamines are required for phospholipase C-{gamma}1 expression promoting intestinal epithelial restitution after wounding Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G335 - G343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shao, J. Chou, C. J. Baty, N. A. Burke, S. C. Watkins, D. B. Stolz, and A. Wells Spatial Localization of m-Calpain to the Plasma Membrane by Phosphoinositide Biphosphate Binding during Epidermal Growth Factor Receptor-Mediated Activation. Mol. Cell. Biol., July 1, 2006; 26(14): 5481 - 5496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ahmed, S. Maines-Bandiera, M. A. Quinn, W. G. Unger, S. Dedhar, and N. Auersperg Molecular pathways regulating EGF-induced epithelio-mesenchymal transition in human ovarian surface epithelium Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1532 - C1542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Orth, E. W. Krueger, S. G. Weller, and M. A. McNiven A novel endocytic mechanism of epidermal growth factor receptor sequestration and internalization. Cancer Res., April 1, 2006; 66(7): 3603 - 3610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Boxall, S. T. Holgate, and D. E. Davies The contribution of transforming growth factor-{beta} and epidermal growth factor signalling to airway remodelling in chronic asthma Eur. Respir. J., January 1, 2006; 27(1): 208 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Aguirre, T. A. Rizvi, N. Ratner, and V. Gallo Overexpression of the Epidermal Growth Factor Receptor Confers Migratory Properties to Nonmigratory Postnatal Neural Progenitors J. Neurosci., November 30, 2005; 25(48): 11092 - 11106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Moh, C. Zhang, C. Luo, L. H. Lee, and S. Shen Structural and Functional Analyses of a Novel Ig-like Cell Adhesion Molecule, hepaCAM, in the Human Breast Carcinoma MCF7 Cells J. Biol. Chem., July 22, 2005; 280(29): 27366 - 27374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Satish, H. C. Blair, A. Glading, and A. Wells Interferon-Inducible Protein 9 (CXCL11)-Induced Cell Motility in Keratinocytes Requires Calcium Flux-Dependent Activation of {micro}-Calpain Mol. Cell. Biol., March 1, 2005; 25(5): 1922 - 1941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sun, P. Gao, L. Chen, D. Ma, J. Wang, J. J. Oppenheim, and N. Zhang Protein Kinase C {zeta} Is Required for Epidermal Growth Factor-Induced Chemotaxis of Human Breast Cancer Cells Cancer Res., February 15, 2005; 65(4): 1433 - 1441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Vaidya, R. M. Ray, and L. R. Johnson MEK1 restores migration of polyamine-depleted cells by retention and activation of Rac1 in the cytoplasm Am J Physiol Cell Physiol, February 1, 2005; 288(2): C350 - C359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Kurten, P. Chowdhury, R. C. Sanders Jr., L. M. Pittman, L. W. Sessions, T. C. Chambers, C. S. Lyle, B. J. Schnackenberg, and S. M. Jones Coordinating epidermal growth factor-induced motility promotes efficient wound closure Am J Physiol Cell Physiol, January 1, 2005; 288(1): C109 - C121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Moran, D. H. Hunt, S. H. Javid, M. Redston, A. M. Carothers, and M. M. Bertagnolli Apc Deficiency Is Associated with Increased Egfr Activity in the Intestinal Enterocytes and Adenomas of C57BL/6J-Min/+ Mice J. Biol. Chem., October 8, 2004; 279(41): 43261 - 43272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Mruk and C. Y. Cheng Sertoli-Sertoli and Sertoli-Germ Cell Interactions and Their Significance in Germ Cell Movement in the Seminiferous Epithelium during Spermatogenesis Endocr. Rev., October 1, 2004; 25(5): 747 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Westhoff, B. Serrels, V. J. Fincham, M. C. Frame, and N. O. Carragher Src-Mediated Phosphorylation of Focal Adhesion Kinase Couples Actin and Adhesion Dynamics to Survival Signaling Mol. Cell. Biol., September 15, 2004; 24(18): 8113 - 8133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Huang, K. Jacobson, and M. D. Schaller MAP kinases and cell migration J. Cell Sci., September 15, 2004; 117(20): 4619 - 4628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Zhuang, Y. Dang, and R. G. Schnellmann Requirement of the epidermal growth factor receptor in renal epithelial cell proliferation and migration Am J Physiol Renal Physiol, September 1, 2004; 287(3): F365 - F372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Rintelen, E. Hafen, and K. Nairz The Drosophila dual-specificity ERK phosphatase DMKP3 cooperates with the ERK tyrosine phosphatase PTP-ER Development, August 1, 2003; 130(15): 3479 - 3490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Churin, L. Al-Ghoul, O. Kepp, T. F. Meyer, W. Birchmeier, and M. Naumann Helicobacter pylori CagA protein targets the c-Met receptor and enhances the motogenic response J. Cell Biol., April 28, 2003; 161(2): 249 - 255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schenk, E. Hintermann, M. Bilban, N. Koshikawa, C. Hojilla, R. Khokha, and V. Quaranta Binding to EGF receptor of a laminin-5 EGF-like fragment liberated during MMP-dependent mammary gland involution J. Cell Biol., April 14, 2003; 161(1): 197 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Tamas, Z. Solti, P. Bauer, A. Illes, S. Sipeki, A. Bauer, A. Farago, J. Downward, and L. Buday Mechanism of Epidermal Growth Factor Regulation of Vav2, a Guanine Nucleotide Exchange Factor for Rac J. Biol. Chem., February 7, 2003; 278(7): 5163 - 5171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sajid, K. V. Vijayan, S. Souza, and P. F. Bray PlA Polymorphism of Integrin {beta}3 Differentially Modulates Cellular Migration on Extracellular Matrix Proteins Arterioscler Thromb Vasc Biol, December 1, 2002; 22(12): 1984 - 1989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-Y. Chen, J. Lu, Y.-C. Shih, and C.-H. Tsai Epstein-Barr Virus Latent Membrane Protein 2A Regulates c-Jun Protein through Extracellular Signal-Regulated Kinase J. Virol., August 12, 2002; 76(18): 9556 - 9561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tanimura, K. Nomura, K.-i. Ozaki, M. Tsujimoto, T. Kondo, and M. Kohno Prolonged Nuclear Retention of Activated Extracellular Signal-regulated Kinase 1/2 Is Required for Hepatocyte Growth Factor-induced Cell Motility J. Biol. Chem., July 26, 2002; 277(31): 28256 - 28264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Edme, J. Downward, J.-P. Thiery, and B. Boyer Ras induces NBT-II epithelial cell scattering through the coordinate activities of Rac and MAPK pathways J. Cell Sci., June 15, 2002; 115(12): 2591 - 2601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Orr, M. A. Pallero, and J. E. Murphy-Ullrich Thrombospondin Stimulates Focal Adhesion Disassembly through Gi- and Phosphoinositide 3-Kinase-dependent ERK Activation J. Biol. Chem., May 31, 2002; 277(23): 20453 - 20460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shiraha, A. Glading, J. Chou, Z. Jia, and A. Wells Activation of m-Calpain (Calpain II) by Epidermal Growth Factor Is Limited by Protein Kinase A Phosphorylation of m-Calpain Mol. Cell. Biol., April 15, 2002; 22(8): 2716 - 2727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Caric, H. Raphael, J. Viti, A. Feathers, D. Wancio, and L. Lillien EGFRs mediate chemotactic migration in the developing telencephalon Development, November 1, 2001; 128(21): 4203 - 4216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Berrou and M. Bryckaert Platelet-derived Growth Factor Inhibits Smooth Muscle Cell Adhesion to Fibronectin by ERK-dependent and ERK-independent Pathways J. Biol. Chem., October 12, 2001; 276(42): 39303 - 39309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Lu, G. Jiang, P. Blume-Jensen, and T. Hunter Epidermal Growth Factor-Induced Tumor Cell Invasion and Metastasis Initiated by Dephosphorylation and Downregulation of Focal Adhesion Kinase Mol. Cell. Biol., June 15, 2001; 21(12): 4016 - 4031. [Abstract] [Full Text]
|
|
|
|
|
|
D. F. Kucik, T. E. O'Toole, A. Zheleznyak, D. K. Busettini, and E. J. Brown Activation-enhanced {alpha}IIb{beta}3-Integrin-Cytoskeleton Interactions Outside of Focal Contacts Require the {alpha}-Subunit Mol. Biol. Cell, May 1, 2001; 12(5): 1509 - 1518. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Kutz, J. Hordines, P. J. McKeown-Longo, and P. J. Higgins TGF-{beta}1-induced PAI-1 gene expression requires MEK activity and cell-to-substrate adhesion J. Cell Sci., January 11, 2001; 114(21): 3905 - 3914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. P. Liu and K. Burridge Vav2 Activates Rac1, Cdc42, and RhoA Downstream from Growth Factor Receptors but Not beta 1 Integrins Mol. Cell. Biol., October 1, 2000; 20(19): 7160 - 7169. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-N. Kim, G. J. Wiepz, A. G. Guadarrama, and P. J. Bertics Epidermal Growth Factor-stimulated Tyrosine Phosphorylation of Caveolin-1. ENHANCED CAVEOLIN-1 TYROSINE PHOSPHORYLATION FOLLOWING ABERRANT EPIDERMAL GROWTH FACTOR RECEPTOR STATUS J. Biol. Chem., March 10, 2000; 275(11): 7481 - 7491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Glading, P. Chang, D. A. Lauffenburger, and A. Wells Epidermal Growth Factor Receptor Activation of Calpain Is Required for Fibroblast Motility and Occurs via an ERK/MAP Kinase Signaling Pathway J. Biol. Chem., January 28, 2000; 275(4): 2390 - 2398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G Maheshwari, G Brown, D. Lauffenburger, A Wells, and L. Griffith Cell adhesion and motility depend on nanoscale RGD clustering J. Cell Sci., January 5, 2000; 113(10): 1677 - 1686. [Abstract] [PDF]
|
|
|
|
|
|
D. Webb, D. Nguyen, and S. Gonias Extracellular signal-regulated kinase functions in the urokinase receptor-dependent pathway by which neutralization of low density lipoprotein receptor-related protein promotes fibrosarcoma cell migration and matrigel invasion J. Cell Sci., January 1, 2000; 113(1): 123 - 134. [Abstract] [PDF]
|
|
|
|
|
|
J. Leng, R. L. Klemke, A. C. Reddy, and D. A. Cheresh Potentiation of Cell Migration by Adhesion-dependent Cooperative Signals from the GTPase Rac and Raf Kinase J. Biol. Chem., December 31, 1999; 274(53): 37855 - 37861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Schaefer, H. Kamiguchi, E. V. Wong, C. M. Beach, G. Landreth, and V. Lemmon Activation of the MAPK Signal Cascade by the Neural Cell Adhesion Molecule L1 Requires L1 Internalization J. Biol. Chem., December 31, 1999; 274(53): 37965 - 37973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Price, T. Tiganis, A. Agarwal, D. Djakiew, and E. W. Thompson Epidermal Growth Factor Promotes MDA-MB-231 Breast Cancer Cell Migration through a Phosphatidylinositol 3'-Kinase and Phospholipase C-dependent Mechanism Cancer Res., November 1, 1999; 59(21): 5475 - 5478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Slack, A. D. Catling, S. T. Eblen, M. J. Weber, and J. T. Parsons c-Raf-mediated Inhibition of Epidermal Growth Factor-stimulated Cell Migration J. Biol. Chem., September 17, 1999; 274(38): 27177 - 27184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Rabinovitz, A. Toker, and A. M. Mercurio Protein Kinase C-Dependent Mobilization of the {alpha}6{beta}4 Integrin from Hemidesmosomes and Its Association with Actin-Rich Cell Protrusions Drive the Chemotactic Migration of Carcinoma Cells J. Cell Biol., September 6, 1999; 146(5): 1147 - 1160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Adelsman, J. B. McCarthy, and Y. Shimizu Stimulation of beta 1-Integrin Function by Epidermal Growth Factor and Heregulin-beta Has Distinct Requirements for erbB2 but a Similar Dependence on Phosphoinositide 3-OH Kinase Mol. Biol. Cell, September 1, 1999; 10(9): 2861 - 2878. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Kassis, J. Moellinger, H. Lo, N. M. Greenberg, H.-G. Kim, and A. Wells A Role for Phospholipase C-{{gamma}}-mediated Signaling in Tumor Cell Invasion Clin. Cancer Res., August 1, 1999; 5(8): 2251 - 2260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H.D. Nguyen, A. D. Catling, D. J. Webb, M. Sankovic, L. A. Walker, A. V. Somlyo, M. J. Weber, and S. L. Gonias Myosin Light Chain Kinase Functions Downstream of Ras/ERK to Promote Migration of Urokinase-Type Plasminogen Activator-Stimulated Cells in an Integrin-Selective Manner J. Cell Biol., July 12, 1999; 146(1): 149 - 164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shiraha, A. Glading, K. Gupta, and A. Wells Ip-10 Inhibits Epidermal Growth Factor-Induced Motility by Decreasing Epidermal Growth Factor Receptor-Mediated Calpain Activity J. Cell Biol., July 12, 1999; 146(1): 243 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li, M.-L. Lin, G. J. Wiepz, A. G. Guadarrama, and P. J. Bertics Integrin-mediated Migration of Murine B82L Fibroblasts Is Dependent on the Expression of an Intact Epidermal Growth Factor Receptor J. Biol. Chem., April 16, 1999; 274(16): 11209 - 11219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhao, A. Dick, J. V. Forrester, and C. D. McCaig Electric Field-directed Cell Motility Involves Up-regulated Expression and Asymmetric Redistribution of the Epidermal Growth Factor Receptors and Is Enhanced by Fibronectin and Laminin Mol. Biol. Cell, April 1, 1999; 10(4): 1259 - 1276. [Abstract] [Full Text]
|
|
|
|
|
|
K. Fang, E Ionides, G Oster, R Nuccitelli, and R. Isseroff Epidermal growth factor receptor relocalization and kinase activity are necessary for directional migration of keratinocytes in DC electric fields J. Cell Sci., January 6, 1999; 112(12): 1967 - 1978. [Abstract] [PDF]
|
|
|
|
|
|
M. Ware, A Wells, and D. Lauffenburger Epidermal growth factor alters fibroblast migration speed and directional persistence reciprocally and in a matrix-dependent manner J. Cell Sci., January 8, 1998; 111(16): 2423 - 2432. [Abstract] [PDF]
|
|
|
|
|
|
H. Shiraha, K. Gupta, K. Drabik, and A. Wells Aging Fibroblasts Present Reduced Epidermal Growth Factor (EGF) Responsiveness Due to Preferential Loss of EGF Receptors J. Biol. Chem., June 16, 2000; 275(25): 19343 - 19351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Glading, F. Uberall, S. M. Keyse, D. A. Lauffenburger, and A. Wells Membrane Proximal ERK Signaling Is Required for M-calpain Activation Downstream of Epidermal Growth Factor Receptor Signaling J. Biol. Chem., June 22, 2001; 276(26): 23341 - 23348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Karihaloo, D. A. O'Rourke, C. Nickel, K. Spokes, and L. G. Cantley Differential MAPK Pathways Utilized for HGF- and EGF-dependent Renal Epithelial Morphogenesis J. Biol. Chem., March 16, 2001; 276(12): 9166 - 9173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. D. Nguyen, D. J. Webb, A. D. Catling, Q. Song, A. Dhakephalkar, M. J. Weber, K. S. Ravichandran, and S. L. Gonias Urokinase-type Plasminogen Activator Stimulates the Ras/Extracellular Signal-regulated Kinase (ERK) Signaling Pathway and MCF-7 Cell Migration by a Mechanism That Requires Focal Adhesion Kinase, Src, and Shc. RAPID DISSOCIATION OF GRB2/SOS-SHC COMPLEX IS ASSOCIATED WITH THE TRANSIENT PHOSPHORYLATION OF ERK IN UROKINASE-TREATED CELLS J. Biol. Chem., June 16, 2000; 275(25): 19382 - 19388. [Abstract] [Full Text] [PDF]
|
|
