|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 6 793-802, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
D Dogic, P Rousselle and M Aumailley
Institut de Biologie et Chimie des Proteines, CNRS UPR 412, Lyon, France.
Laminin 1 (alpha1beta1gamma1) and laminin 5 (alpha3beta3gamma2) induce cell adhesion with different involvement of integrins: both are ligands for the alpha6beta1 integrin, while alpha3beta1 integrin has affinity for laminin 5 only. These two laminin isoforms therefore provide good models to investigate whether alpha3beta1 and alpha6beta1 integrins play different roles in signal transduction and in focal adhesion formation. Laminin 1 or 5 induced adhesion of normal human skin fibroblasts to a similar extent but promoted different overall cell shapes. On laminin 1 the fibroblasts formed mainly filopodia-like structures, while on laminin 5 they developed lamellipodias. Staining of fibrillar actin with fluorescein-phalloidin revealed a similar organisation of the actin cytoskeleton on both substrates. However, integrin subunits and several cytoskeletal linker proteins, including vinculin, talin, and paxillin, showed an isoform-specific arrangement into focal adhesions. On laminin 1 they were recruited into thick and short aggregates localized at the termini of actin stress fibers, while on laminin 5 they appeared as dots or streaks clustered on a long portion of actin microfilaments. To test whether the differing affinity of laminin 1 or 5 for alpha3beta1 integrin would explain the formation of morphologically different focal adhesions, cells were seeded on laminin 1 under conditions in which alpha3beta1 integrins were occupied by a function-blocking antibody. This resulted in the formation of focal adhesions similar to that observed on laminin 5, where the integrin is occupied by its natural ligand. These results provide the first evidence for a cross-talk between alpha3beta1 and alpha6beta1 integrins and indicate that occupancy of alpha3beta1 integrins results in a trans-dominant regulation of alpha6beta1 integrin clustering and of focal adhesions. It suggests that recruitment of integrins and cytoskeletal linker proteins are laminin isoform-specific and that tissue specific expression of laminin isoforms might modulate cell behavior by the activation of distinct sets of integrins and by the induction of distinct molecular assemblies within the cell adhesion signaling complexes.
This article has been cited by other articles:
|
|
 |
|
  Y. Minami, W. Ikeda, M. Kajita, T. Fujito, H. Amano, Y. Tamaru, K. Kuramitsu, Y. Sakamoto, M. Monden, and Y. Takai Necl-5/Poliovirus Receptor Interacts in cis with Integrin {alpha}Vbeta3 and Regulates Its Clustering and Focal Complex Formation J. Biol. Chem., June 22, 2007; 282(25): 18481 - 18496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Lavigne, X. M. O'Brien, M. Kim, J. W. Janowski, J. E. Albina, and J. S. Reichner Integrin Engagement Mediates the Human Polymorphonuclear Leukocyte Response to a Fungal Pathogen-Associated Molecular Pattern J. Immunol., June 1, 2007; 178(11): 7276 - 7282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hozumi, N. Suzuki, P. K. Nielsen, M. Nomizu, and Y. Yamada Laminin {alpha}1 Chain LG4 Module Promotes Cell Attachment through Syndecans and Cell Spreading through Integrin {alpha}2beta1 J. Biol. Chem., October 27, 2006; 281(43): 32929 - 32940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Joly, V. Morel, A. Hummel, A. Ruello, P. Nusbaum, N. Patey, L.-H. Noel, P. Rousselle, and B. Knebelmann {beta}4 Integrin and Laminin 5 Are Aberrantly Expressed in Polycystic Kidney Disease: Role in Increased Cell Adhesion and Migration Am. J. Pathol., November 1, 2003; 163(5): 1791 - 1800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Zhang, C. C. Tom, M. C. Kugler, T.-T. Ching, J. A. Kreidberg, Y. Wei, and H. A. Chapman Distinct ligand binding sites in integrin {alpha}3{beta}1 regulate matrix adhesion and cell-cell contact J. Cell Biol., October 13, 2003; 163(1): 177 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ferletta, Y. Kikkawa, H. Yu, J. F. Talts, M. Durbeej, A. Sonnenberg, R. Timpl, K. P. Campbell, P. Ekblom, and E. Genersch Opposing Roles of Integrin {alpha}6A{beta}1 and Dystroglycan in Laminin-mediated Extracellular Signal-regulated Kinase Activation Mol. Biol. Cell, May 1, 2003; 14(5): 2088 - 2103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Grace and J. Busciglio Aberrant Activation of Focal Adhesion Proteins Mediates Fibrillar Amyloid beta -Induced Neuronal Dystrophy J. Neurosci., January 15, 2003; 23(2): 493 - 502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. G. Crean, D. Finlay, M. Murphy, C. Moss, C. Godson, F. Martin, and H. R. Brady The Role of p42/44 MAPK and Protein Kinase B in Connective Tissue Growth Factor Induced Extracellular Matrix Protein Production, Cell Migration, and Actin Cytoskeletal Rearrangement in Human Mesangial Cells J. Biol. Chem., November 8, 2002; 277(46): 44187 - 44194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, H. Miao, S. Li, K.-D. Chen, Y.-S. Li, S. Yuan, J. Y.-J. Shyy, and S. Chien Interplay between integrins and FLK-1 in shear stress-induced signaling Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1540 - C1547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Steketee and K. W. Tosney Three Functionally Distinct Adhesions in Filopodia: Shaft Adhesions Control Lamellar Extension J. Neurosci., September 15, 2002; 22(18): 8071 - 8083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Gout, M. R. Jacquier-Sarlin, L. Rouard-Talbot, P. Rousselle, and M. R. Block RhoA-dependent Switch between alpha 2beta 1 and alpha 3beta 1 Integrins Is Induced by Laminin-5 during Early Stage of HT-29 Cell Differentiation Mol. Biol. Cell, October 1, 2001; 12(10): 3268 - 3281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Yanez-Mo, R Tejedor, P Rousselle, and F Sanchez -Madrid Tetraspanins in intercellular adhesion of polarized epithelial cells: spatial and functional relationship to integrins and cadherins J. Cell Sci., January 2, 2001; 114(3): 577 - 587. [Abstract] [PDF]
|
|
|
|
|
|
B.-Z. Katz, E. Zamir, A. Bershadsky, Z. Kam, K. M. Yamada, and B. Geiger Physical State of the Extracellular Matrix Regulates the Structure and Molecular Composition of Cell-Matrix Adhesions Mol. Biol. Cell, March 1, 2000; 11(3): 1047 - 1060. [Abstract] [Full Text]
|
|
|
|
|
|
E Laplantine, L Vallar, K Mann, N Kieffer, and M Aumailley Interaction between the cytodomains of the alpha 3 and beta 1 integrin subunits regulates remodelling of adhesion complexes on laminin J. Cell Sci., January 4, 2000; 113(7): 1167 - 1176. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Pasco, J. Han, P. Gillery, G. Bellon, F.-X. Maquart, J. P. Borel, N. A. Kefalides, and J. C. Monboisse A Specific Sequence of the Noncollagenous Domain of the {{alpha}}3(IV) Chain of Type IV Collagen Inhibits Expression and Activation of Matrix Metalloproteinases by Tumor Cells Cancer Res., January 1, 2000; 60(2): 467 - 473. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. T Konttinen, T. F. Li, J. W. Xu, M. Tagaki, L. Pirilä, T. Silvennoinen, S. Santavirta, and I. Virtanen Expression of laminins and their integrin receptors in different conditions of synovial membrane and synovial membrane-like interface tissue Ann Rheum Dis, November 1, 1999; 58(11): 683 - 690. [Abstract] [Full Text]
|
|
|
|
|
|
M Grassi, G Moens, P Rousselle, J. Thiery, and J Jouanneau The SFL activity secreted by metastatic carcinoma cells is related to laminin 5 and mediates cell scattering in an integrin-independent manner J. Cell Sci., January 8, 1999; 112(15): 2511 - 2520. [Abstract] [PDF]
|
|
|
|
|
|
M Ferletta and P Ekblom Identification of laminin-10/11 as a strong cell adhesive complex for a normal and a malignant human epithelial cell line J. Cell Sci., January 1, 1999; 112(1): 1 - 10. [Abstract] [PDF]
|
|
|
|
|
|
V. Wixler, D. Geerts, E. Laplantine, D. Westhoff, N. Smyth, M. Aumailley, A. Sonnenberg, and M. Paulsson The LIM-only Protein DRAL/FHL2 Binds to the Cytoplasmic Domain of Several alpha and beta Integrin Chains and Is Recruited to Adhesion Complexes J. Biol. Chem., October 20, 2000; 275(43): 33669 - 33678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-C. Chen, N. Chen, and L. F. Lau The Angiogenic Factors Cyr61 and Connective Tissue Growth Factor Induce Adhesive Signaling in Primary Human Skin Fibroblasts J. Biol. Chem., March 23, 2001; 276(13): 10443 - 10452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fujiwara, Y. Kikkawa, N. Sanzen, and K. Sekiguchi Purification and Characterization of Human Laminin-8. LAMININ-8 STIMULATES CELL ADHESION AND MIGRATION THROUGH alpha 3beta 1 AND alpha 6beta 1 INTEGRINS J. Biol. Chem., May 11, 2001; 276(20): 17550 - 17558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Sinclair and A. D. O'Brien Cell Surface-localized Nucleolin Is a Eukaryotic Receptor for the Adhesin Intimin-gamma of Enterohemorrhagic Escherichia coli O157:H7 J. Biol. Chem., January 18, 2002; 277(4): 2876 - 2885. [Abstract] [Full Text] [PDF]
|
|
