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Journal of Cell Science, Vol 111, Issue 6 815-823, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
A Pierce, AD Whetton, PJ Owen-Lynch, J Tavernier, E Spooncer, TM Dexter and CM Heyworth
Leukemia Research Fund, Cellular Development Unit, UMIST, Manchester M60 1QD, UK.
The interleukin-5 (IL-5) receptor is a heterodimer that consists of an IL-5 specific alpha subunit and a common ssc chain that is shared with the receptors for granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-3 (IL-3). In contrast to IL-5, which acts mainly as an eosinophil lineage specific factor in vivo, IL-3 and GM-CSF stimulate the survival, proliferation and development of various hematopoietic cell lineages and also multipotent progenitor cells. IL-5 has little effect on the survival or proliferation of the multipotent stem cell line FDCP-Mix A4 but does promote some eosinophil development. To investigate whether the lineage specificity of IL-5 is due to the restricted expression of the IL-5 receptor alpha subunit we transfected the FDCP-Mix A4 cells with a retroviral vector containing this alpha subunit. The ectopic expression of the IL-5 receptor alpha subunit in the FDCP-Mix cells did not increase the observed eosinophilic development but did stimulate survival and proliferation of the transfected cells when IL-5 was added. IL-5 thus acts like IL-3 in these cells, promoting proliferation and survival. The results suggest that IL-5, whilst having a capacity to promote proliferation, does not influence eosinophilic lineage commitment in these multipotent cells. The results further argue that the observed lineage specificity of IL-5 is probably due to factors in addition to the restricted expression of the IL-5 receptor alpha subunit.
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  R. D. Unwin, D. W. Sternberg, Y. Lu, A. Pierce, D. G. Gilliland, and A. D. Whetton Global Effects of BCR/ABL and TEL/PDGFR{beta} Expression on the Proteome and Phosphoproteome: IDENTIFICATION OF THE RHO PATHWAY AS A TARGET OF BCR/ABL J. Biol. Chem., February 25, 2005; 280(8): 6316 - 6326. [Abstract] [Full Text] [PDF]
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 L. Bruno, R. Hoffmann, F. McBlane, J. Brown, R. Gupta, C. Joshi, S. Pearson, T. Seidl, C. Heyworth, and T. Enver Molecular Signatures of Self-Renewal, Differentiation, and Lineage Choice in Multipotential Hemopoietic Progenitor Cells In Vitro Mol. Cell. Biol., January 15, 2004; 24(2): 741 - 756. [Abstract] [Full Text] [PDF]
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 C. A. Evans, S. Ariffin, A. Pierce, and A. D. Whetton Identification of primary structural features that define the differential actions of IL-3 and GM-CSF receptors Blood, October 16, 2002; 100(9): 3164 - 3174. [Abstract] [Full Text] [PDF]
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J. Tavernier, J. Van der Heyden, A. Verhee, G. Brusselle, X. Van Ostade, J. Vandekerckhove, J. North, S. M. Rankin, A. B. Kay, and D. S. Robinson Interleukin 5 regulates the isoform expression of its own receptor alpha -subunit Blood, March 1, 2000; 95(5): 1600 - 1607. [Abstract] [Full Text] [PDF]
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J. M. Francis, C. M. Heyworth, E. Spooncer, A. Pierce, T. M. Dexter, and A. D. Whetton Transforming Growth Factor-beta 1 Induces Apoptosis Independently of p53 and Selectively Reduces Expression of Bcl-2 in Multipotent Hematopoietic Cells J. Biol. Chem., December 8, 2000; 275(50): 39137 - 39145. [Abstract] [Full Text] [PDF]
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