|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 7 853-865, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
DR Drummond and IM Hagan
School of Biological Sciences, University of Manchester, Manchester, UK.
Members of the bimC family of kinesin related proteins (KRPs) play vital roles in the formation and function of the mitotic spindle. Although they share little amino acid homology outside the highly conserved microtubule motor domain, several family members do contain a 'bimC box', a sequence motif around a p34(cdc2) consensus phosphorylation site in their carboxy-terminal 'tail' region. One family member, Eg5, requires phosphorylation at this site for association with the mitotic spindle. We show that mutations in the Schizosaccharomyces pombe cut7+ gene that change the bimC box p34(cdc2) consensus phosphorylation site at position 1,011 and a neighbouring MAP kinase consensus phosphorylation site at position 1,020 to non-phosphorylatable residues did not affect the ability of S. pombe cut7 genes to complement temperature sensitive cut7 mutants. Phosphorylation site mutants expressed as fusions to green fluorescent protein associated with the mitotic spindle with a localisation indistinguishable from similarly expressed wild-type Cut7. Cells in which cut7.T1011A replaced the genomic copy of cut7+ were viable and formed normal spindles. Deletion of the entire carboxy-terminal tail region did not affect the ability of Cut7 to associate with the mitotic spindle but did inhibit normal spindle formation. Thus, unlike Eg5, neither the p34(cdc2) consensus phosphorylation site in the bimC box nor the entire tail region of Cut7 are required for association with the mitotic spindle.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Sarmah and S. R. Wente Dual Functions for the Schizosaccharomyces pombe Inositol Kinase Ipk1 in Nuclear mRNA Export and Polarized Cell Growth Eukaryot. Cell, February 1, 2009; 8(2): 134 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rapley, M. Nicolas, A. Groen, L. Regue, M. T. Bertran, C. Caelles, J. Avruch, and J. Roig The NIMA-family kinase Nek6 phosphorylates the kinesin Eg5 at a novel site necessary for mitotic spindle formation J. Cell Sci., December 1, 2008; 121(23): 3912 - 3921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Hildebrandt, L. Gheber, T. Kingsbury, and M. A. Hoyt Homotetrameric Form of Cin8p, a Saccharomyces cerevisiae Kinesin-5 Motor, Is Essential for Its in Vivo Function J. Biol. Chem., September 8, 2006; 281(36): 26004 - 26013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Rosenberg, G. C. Tomlin, W. H. McDonald, B. E. Snydsman, E. G. Muller, J. R. Yates III, and K. L. Gould Ppc89 Links Multiple Proteins, Including the Septation Initiation Network, to the Core of the Fission Yeast Spindle-Pole Body Mol. Biol. Cell, September 1, 2006; 17(9): 3793 - 3805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Wilson, R. Simmons, and S. Shigali Novel nuclear defects in KLP61F-deficient mutants in Drosophila are partially suppressed by loss of Ncd function J. Cell Sci., October 1, 2004; 117(21): 4921 - 4933. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. H. MacIver, K. Tanaka, A. M. Robertson, and I. M. Hagan Physical and functional interactions between polo kinase and the spindle pole component Cut12 regulate mitotic commitment in S. pombe Genes & Dev., June 15, 2003; 17(12): 1507 - 1523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Petersen, J. Paris, M. Willer, M. Philippe, and I. M. Hagan The S. pombe aurora-related kinase Ark1 associates with mitotic structures in a stage dependent manner and is required for chromosome segregation J. Cell Sci., March 14, 2002; 114(24): 4371 - 4384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Gordon and D. M. Roof The Kinesin-related Protein Kip1p of Saccharomyces cerevisiae Is Bipolar J. Biol. Chem., October 1, 1999; 274(40): 28779 - 28786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Giet, R. Uzbekov, F. Cubizolles, K. Le Guellec, and C. Prigent The Xenopus laevis Aurora-related Protein Kinase pEg2 Associates with and Phosphorylates the Kinesin-related Protein XlEg5 J. Biol. Chem., May 21, 1999; 274(21): 15005 - 15013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Sharp, K. L. McDonald, H. M. Brown, H. J. Matthies, C. Walczak, R. D. Vale, T. J. Mitchison, and J. M. Scholey The Bipolar Kinesin, KLP61F, Cross-links Microtubules within Interpolar Microtubule Bundles of Drosophila Embryonic Mitotic Spindles J. Cell Biol., January 11, 1999; 144(1): 125 - 138. [Abstract] [Full Text] [PDF]
|
|
