|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 7 985-994, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
JM Fayard, C Tessier, JF Pageaux, M Lagarde and C Laugier
Laboratoire de Biochimie et Pharmacologie, INSERM, Villeurbanne, France.
We have previously demonstrated that pancreatic PLA2 (PLA2-I) stimulates the proliferation of UIII cells, a stromal cell line derived from normal rat uterus. In order to gain further insight into the mechanism of action of PLA2-I, we have investigated the intracellular processing of PLA2-I. Either highly proliferative or growth arrested UIII cells were analyzed. Growth arrested cells were obtained from a contact inhibited monolayer or from aristolochic acid-treated cultures. Using cellular fractionation, western blotting, immunocytochemistry and confocal microscopy, we demonstrate that endogenous PLA2-I was mainly located in the nucleus in highly proliferative cells whereas its location was cytoplasmic in non proliferative cells. When non confluent UIII cells were incubated with nanomolar amounts of exogenous PLA2-I, the enzyme was internalized and, in the majority of cells, appeared within the nucleus. Both internalization and nuclear location of exogenous PLA2-I were suppressed by the addition of aristolochic acid to the culture medium. Binding experiments performed on purified nuclear preparations showed the presence of specific cooperative binding sites for PLA2-I. Collectively our data suggest that the proliferative effect exerted by pancreatic PLA2 in UIII cells is mediated by a direct interaction of the enzyme at the nuclear level. Putative mechanisms and targets are discussed.
This article has been cited by other articles:
|
|
 |
|
  F. Gobeil Jr., S. G. Bernier, A. Vazquez-Tello, S. Brault, M. H. Beauchamp, C. Quiniou, A. M. Marrache, D. Checchin, F. Sennlaub, X. Hou, et al. Modulation of Pro-inflammatory Gene Expression by Nuclear Lysophosphatidic Acid Receptor Type-1 J. Biol. Chem., October 3, 2003; 278(40): 38875 - 38883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Beck, G. Lambeau, K. Scholz-Pedretti, M. H. Gelb, M. J. W. Janssen, S. H. Edwards, D. C. Wilton, J. Pfeilschifter, and M. Kaszkin Potentiation of Tumor Necrosis Factor {alpha}-induced Secreted Phospholipase A2 (sPLA2)-IIA Expression in Mesangial Cells by an Autocrine Loop Involving sPLA2 and Peroxisome Proliferator-activated Receptor {alpha} Activation J. Biol. Chem., August 8, 2003; 278(32): 29799 - 29812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. N. Re Intracellular Renin and the Nature of Intracrine Enzymes Hypertension, August 1, 2003; 42(2): 117 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Irvine Nuclear Lipid Signaling Sci. Signal., September 17, 2002; 2002(150): re13 - re13. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Gilmour and M. D. Mitchell Nuclear Lipid Signaling: Novel Role of Eicosanoids Experimental Biology and Medicine, January 1, 2001; 226(1): 1 - 4. [Abstract] [Full Text]
|
|
|
|
|
|
M. Murakami, T. Kambe, S. Shimbara, S. Yamamoto, H. Kuwata, and I. Kudo Functional Association of Type IIA Secretory Phospholipase A2 with the Glycosylphosphatidylinositol-anchored Heparan Sulfate Proteoglycan in the Cyclooxygenase-2-mediated Delayed Prostanoid-biosynthetic Pathway J. Biol. Chem., October 15, 1999; 274(42): 29927 - 29936. [Abstract] [Full Text] [PDF]
|
|
