Journal of Cell Science, Vol 111, Issue 8 1061-1069, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

A novel Rab GTPase, Rab33B, is ubiquitously expressed and localized to the medial Golgi cisternae

JY Zheng, T Koda, T Fujiwara, M Kishi, Y Ikehara and M Kakinuma
Section of Bacterial Infection, Institute of Immunological Science, Hokkaido University, Sapporo, Japan.

Small GTP-binding proteins of the Rab family play important roles at defined steps of vesicular transport in protein secretion and the endocytosis pathway. In mammals, more than 30 proteins belonging to the Rab family have been reported to date. We report here the molecular cloning and characterization of a novel Rab protein, Rab33B. The amino acid sequence of Rab33B shows 55.3% identity to the Rab33A protein (previously called S10), and these two proteins share unique amino acid sequences at the effector domain. The genomic organization of rab33B was the same as rab33A: it consists of two exons. Thus, these two proteins make a subclass within the Rab family. Northern blot analysis showed that rab33B is expressed ubiquitously in mouse tissues, in contrast to rab33A whose expression is restricted to the brain and the immune system. A 26 kDa protein was detected by western blotting using a Rab33B-specific monoclonal antibody. Using immunofluorescence studies, Rab33B was shown to co-localize with (alpha)-mannosidase II, a Golgi-specific marker. Immunoelectron microscopy analysis further defined the localization of Rab33B to the medial Golgi cisternae. These results suggest Rab33B plays a role in intra-Golgi transport.

This article has been cited by other articles:

				G. L. Hayes, F. C. Brown, A. K. Haas, R. M. Nottingham, F. A. Barr, and S. R. Pfeffer Multiple Rab GTPase Binding Sites in GCC185 Suggest a Model for Vesicle Tethering at the Trans-Golgi Mol. Biol. Cell, January 1, 2009; 20(1): 209 - 217. [Abstract] [Full Text] [PDF]

				S. Y. Dejgaard, A. Murshid, A. Erman, O. Kizilay, D. Verbich, R. Lodge, K. Dejgaard, T. B. N. Ly-Hartig, R. Pepperkok, J. C. Simpson, et al. Rab18 and Rab43 have key roles in ER-Golgi trafficking J. Cell Sci., August 15, 2008; 121(16): 2768 - 2781. [Abstract] [Full Text] [PDF]

				T. Itoh, N. Fujita, E. Kanno, A. Yamamoto, T. Yoshimori, and M. Fukuda Golgi-resident Small GTPase Rab33B Interacts with Atg16L and Modulates Autophagosome Formation Mol. Biol. Cell, July 1, 2008; 19(7): 2916 - 2925. [Abstract] [Full Text] [PDF]

				T. Tsuboi and M. Fukuda Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells J. Cell Sci., June 1, 2006; 119(11): 2196 - 2203. [Abstract] [Full Text] [PDF]

				C. Gurkan, H. Lapp, C. Alory, A. I. Su, J. B. Hogenesch, and W. E. Balch Large-Scale Profiling of Rab GTPase Trafficking Networks: The Membrome Mol. Biol. Cell, August 1, 2005; 16(8): 3847 - 3864. [Abstract] [Full Text] [PDF]

				J. Young, T. Stauber, E. del Nery, I. Vernos, R. Pepperkok, and T. Nilsson Regulation of Microtubule-dependent Recycling at the Trans-Golgi Network by Rab6A and Rab6A' Mol. Biol. Cell, January 1, 2005; 16(1): 162 - 177. [Abstract] [Full Text] [PDF]

				D. J. Strick, D. M. Francescutti, Y. Zhao, and L. A. Elferink Mammalian Suppressor of Sec4 Modulates the Inhibitory Effect of Rab15 during Early Endocytosis J. Biol. Chem., August 30, 2002; 277(36): 32722 - 32729. [Abstract] [Full Text] [PDF]

				F. Opdam, A Echard, H. Croes, J. van den Hurk, R. van de Vorstenbosch, L. Ginsel, B Goud, and J. Fransen The small GTPase Rab6B, a novel Rab6 subfamily member, is cell-type specifically expressed and localised to the Golgi apparatus J. Cell Sci., January 8, 2000; 113(15): 2725 - 2735. [Abstract] [PDF]

				A. Feliciello, A. Gallo, E. Mele, A. Porcellini, G. Troncone, C. Garbi, M. E. Gottesman, and E. V. Avvedimento The Localization and Activity of cAMP-dependent Protein Kinase Affect Cell Cycle Progression in Thyroid Cells J. Biol. Chem., January 7, 2000; 275(1): 303 - 311. [Abstract] [Full Text] [PDF]