|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 8 1081-1093, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
T Endo and B Nadal-Ginard
Department of Biology, Faculty of Science, Chiba University, Chiba, Japan. tendo@cpuhd.nd.chiba-u.ac.jp
Terminally differentiated skeletal muscle myotubes are arrested in the G0 phase of the cell cycle, and this arrest is not reversed by stimulation with serum or growth factors. The myotubes have been shown to be refractory to apoptosis even under low serum conditions. When the SV40 large T antigen is induced in the C2SVTts11 myotubes, which stably harbor the T antigen gene linked to an inducible promoter, the terminally differentiated cells reenter the cell cycle to resume nuclear DNA replication representing S phase. We show here that the large T-expressing myotubes further proceeded to M phase represented by the appearance of mitotic figures with centrosomes, condensed chromosomes, and mitotic spindles. The myotubes eventually cleaved and midbodies were formed at the cleavage sites of the cytoplasm. In some cases actin filaments, reminiscent of the contractile rings, accumulated at the cleavage furrows. Thus, terminally differentiated myotubes remain able to resume at least one round of the cell cycle and consequently are considered to be capable of dedifferentiation. A subset of myotubes expressing large T did not undergo mitosis. Some of them were degenerative and contained deformed giant nuclei and pulverized nuclei. The others suffered apoptotic cell death, which was identified by morphological changes of the nuclei and the labeling with dUTP at the ends of chromatin DNA fragments. The induction of apoptosis was unlikely to be confined to a particular phase of the cell cycle. These results imply that terminally differentiated myotubes also retain a complete set of machinery for apoptosis.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Blais, C. J.C. van Oevelen, R. Margueron, D. Acosta-Alvear, and B. D. Dynlacht Retinoblastoma tumor suppressor protein dependent methylation of histone H3 lysine 27 is associated with irreversible cell cycle exit J. Cell Biol., December 31, 2007; 179(7): 1399 - 1412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Galindo, J. A. Allport, and E. N. Olson A Drosophila model of the rhabdomyosarcoma initiator PAX7-FKHR PNAS, September 5, 2006; 103(36): 13439 - 13444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Lilyestrom, M. G. Klein, R. Zhang, A. Joachimiak, and X. S. Chen Crystal structure of SV40 large T-antigen bound to p53: interplay between a viral oncoprotein and a cellular tumor suppressor Genes & Dev., September 1, 2006; 20(17): 2373 - 2382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Sun, H. Watanabe, K. Takano, T. Yokoyama, J.-i. Fujisawa, and T. Endo Sustained activation of M-Ras induced by nerve growth factor is essential for neuronal differentiation of PC12 cells. Genes Cells, September 1, 2006; 11(9): 1097 - 1113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Dimarakis, N. A. Habib, and M. Y.A. Gordon Adult bone marrow-derived stem cells and the injured heart: just the beginning? Eur. J. Cardiothorac. Surg., November 1, 2005; 28(5): 665 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Abe, K. Takano, A. Suzuki, Y. Shimada, M. Inagaki, N. Sato, T. Obinata, and T. Endo Myocyte differentiation generates nuclear invaginations traversed by myofibrils associating with sarcomeric protein mRNAs J. Cell Sci., December 15, 2004; 117(26): 6523 - 6534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Abe, M. Kato, H. Miki, T. Takenawa, and T. Endo Small GTPase Tc10 and its homologue RhoT induce N-WASP-mediated long process formation and neurite outgrowth J. Cell Sci., January 1, 2003; 116(1): 155 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Grounds, J. D. White, N. Rosenthal, and M. A. Bogoyevitch The Role of Stem Cells in Skeletal and Cardiac Muscle Repair J. Histochem. Cytochem., May 1, 2002; 50(5): 589 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. McGann, S. J. Odelberg, and M. T. Keating Mammalian myotube dedifferentiation induced by newt regeneration extract PNAS, November 20, 2001; 98(24): 13699 - 13704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-M. Loo and T. Melendy The majority of human replication protein A remains complexed throughout the cell cycle Nucleic Acids Res., September 1, 2000; 28(17): 3354 - 3360. [Abstract] [Full Text] [PDF]
|
|
