spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Churchill, G. C.
Right arrow Articles by Louis, C. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Churchill, G. C.
Right arrow Articles by Louis, C. F.

Journal of Cell Science, Vol 111, Issue 9 1217-1225, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

Roles of Ca2+, inositol trisphosphate and cyclic ADP-ribose in mediating intercellular Ca2+ signaling in sheep lens cells

G Churchill and C Louis
Department of Biochemistry, University of Minnesota, St. Paul, MN 55108, USA.

To further characterize how gap junction-dependent Ca2+ waves propagate between sheep lens cells, we examined the possible roles of inositol 1,4,5-trisphosphate (IP3), Ca2+ and cyclic ADP-ribose (cADPR) in mediating intercellular Ca2+ waves. Second messengers were microinjected into a single cell in a monolayer of sheep lens cells while monitoring cytosolic Ca2+ with fura-2 and fluorescence microscopy. All three compounds initiated intercellular Ca2+ waves, but more cells responded following the injection of either IP3 or cADPR than responded following the injection of Ca2+. When either IP3 or cADPR was co-injected with the Ca2+ chelator EGTA, cytosolic Ca2+ in the injected cell decreased but cytosolic Ca2+ in the adjacent cells increased, indicating that the intercellular messenger was IP3 or cADPR, rather than Ca2+. The phospholipase C inhibitor U73122 eliminated mechanically initiated intercellular Ca2+ waves, indicating that mechanical initiation probably requires IP3 production. In U73122-treated cells, injected IP3 initiated an intercellular Ca2+ wave in which the number of cells responding increased as the amount of IP3 injected increased, indicating that the distance traveled by the Ca2+ wave was dependent on cell-to-cell diffusion of IP3. In contrast, the ability of cADPR both to increase cytosolic Ca2+ in the injected cell and to initiate intercellular Ca2+ waves was greatly attenuated by U73122. In conclusion, Ca2+, IP3 and cADPR can all mediate intercellular Ca2+ waves by passing through gap junction channels, but both IP3 and cADPR are more effective intercellular messengers than Ca2+.


This article has been cited by other articles:


Home page
 J. Physiol.Home page
B. Lavoie, O. B. Balemba, M. T. Nelson, S. M. Ward, and G. M. Mawe
Morphological and physiological evidence for interstitial cell of Cajal-like cells in the guinea pig gallbladder
J. Physiol., March 1, 2007; 579(2): 487 - 501.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
F. Vanden Abeele, G. Bidaux, D. Gordienko, B. Beck, Y. V. Panchin, A. V. Baranova, D. V. Ivanov, R. Skryma, and N. Prevarskaya
Functional implications of calcium permeability of the channel formed by pannexin 1
J. Cell Biol., August 14, 2006; 174(4): 535 - 546.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
D. Garcia-Dorado, A. Rodriguez-Sinovas, and M. Ruiz-Meana
Gap junction-mediated spread of cell injury and death during myocardial ischemia-reperfusion
Cardiovasc Res, February 15, 2004; 61(3): 386 - 401.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. M. Lurtz and C. F. Louis
Calmodulin and protein kinase C regulate gap junctional coupling in lens epithelial cells
Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1475 - C1482.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
W. S. Nesbitt, S. Giuliano, S. Kulkarni, S. M. Dopheide, I. S. Harper, and S. P. Jackson
Intercellular calcium communication regulates platelet aggregation and thrombus growth
J. Cell Biol., March 31, 2003; 160(7): 1151 - 1161.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. Yao, T. Morioka, B. Li, and T. Oite
Coordination of Mesangial Cell Contraction by Gap Junction-Mediated Intercellular Ca2+ Wave
J. Am. Soc. Nephrol., August 1, 2002; 13(8): 2018 - 2026.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. Vazquez-Martinez, S. L. Shorte, F. R. Boockfor, and L. S. Frawley
Synchronized Exocytotic Bursts from Gonadotropin-Releasing Hormone-Expressing Cells: Dual Control by Intrinsic Cellular Pulsatility and Gap Junctional Communication
Endocrinology, May 1, 2001; 142(5): 2095 - 2101.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
E. Scemes, S. O. Suadicani, and D. C. Spray
Intercellular Communication in Spinal Cord Astrocytes: Fine Tuning between Gap Junctions and P2 Nucleotide Receptors in Calcium Wave Propagation
J. Neurosci., February 15, 2000; 20(4): 1435 - 1445.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. Ruiz-Meana, D. Garcia-Dorado, B. Hofstaetter, H. M. Piper, and J. Soler-Soler
Propagation of Cardiomyocyte Hypercontracture by Passage of Na+ Through Gap Junctions
Circ. Res., August 6, 1999; 85(3): 280 - 287.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1998