|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 10 1497-1509, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
MA Messerli, G Danuser and KR Robinson
Department of Biological Sciences, West Lafayette, IN 47907-1392, USA.
Fluxes of H+, K+ and Ca2+ were measured with self-referencing ion-selective probes, near the plasma membrane of growing Lilium longiflorum pollen tubes. Measurements from three regions around short, steady-growing tubes showed small, steady influx of H+ over the distal 40 microm and a region of the tube within 50-100 microm of the grain with larger magnitude efflux from the grain. K+ fluxes were immeasurable in short tubes. Measurements of longer tubes that were growing in a pulsatile manner revealed a pulsatile influx of both H+ and K+ at the growing tip. The average fluxes at the cell surface during the peaks of the H+ and K+ pulses were 489+/-81 and 688+/-144 pmol cm-2 second-1, respectively. Growth was measured by tracking the pollen tips with a computer vision system that achieved a spatial resolution of approximately 1/10 pixel. The high spatial resolution enabled the detection of growth, and thus the changes in growth rates, with a temporal sampling rate of 1 frame/second. These data show that the H+ and K+ pulses have a phase lag of 103+/-9 and 100+/-11 degrees, respectively, with respect to the growth pulses. Calcium fluxes were also measured in growing tubes. During steady growth, the calcium influx was relatively steady. When pulsatile growth began, the basal Ca2+ influx decreased and a pulsatile component appeared, superimposed on the reduced basal Ca2+ flux. The peaks of the Ca2+ pulses at the cell surface averaged 38.4+/-2.5 pmol cm-2 second-1. Longer tubes had large pulsatile Ca2+ fluxes with smaller baseline fluxes. The Ca2+ influx pulses had a phase lag of 123+/-9 degrees with respect to the growth pulses.
This article has been cited by other articles:
|
|
 |
|
  G. B. Monshausen, M. A. Messerli, and S. Gilroy Imaging of the Yellow Cameleon 3.6 Indicator Reveals That Elevations in Cytosolic Ca2+ Follow Oscillating Increases in Growth in Root Hairs of Arabidopsis Plant Physiology, August 1, 2008; 147(4): 1690 - 1698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Certal, R. B. Almeida, L. M. Carvalho, E. Wong, N. Moreno, E. Michard, J. Carneiro, J. Rodriguez-Leon, H.-M. Wu, A. Y. Cheung, et al. Exclusion of a Proton ATPase from the Apical Membrane Is Associated with Cell Polarity and Tip Growth in Nicotiana tabacum Pollen Tubes PLANT CELL, March 1, 2008; 20(3): 614 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. B. Monshausen, T. N. Bibikova, M. A. Messerli, C. Shi, and S. Gilroy From the Cover: Oscillations in extracellular pH and reactive oxygen species modulate tip growth of Arabidopsis root hairs PNAS, December 26, 2007; 104(52): 20996 - 21001. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Campanoni and M. R. Blatt Membrane trafficking and polar growth in root hairs and pollen tubes J. Exp. Bot., January 1, 2007; 58(1): 65 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lovy-Wheeler, J. G. Kunkel, E. G. Allwood, P. J. Hussey, and P. K. Hepler Oscillatory Increases in Alkalinity Anticipate Growth and May Regulate Actin Dynamics in Pollen Tubes of Lily PLANT CELL, September 1, 2006; 18(9): 2182 - 2193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-U. Hwang, Y. Gu, Y.-J. Lee, and Z. Yang Oscillatory ROP GTPase Activation Leads the Oscillatory Polarized Growth of Pollen Tubes Mol. Biol. Cell, November 1, 2005; 16(11): 5385 - 5399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Noma, K. Iida, and H. Iida Polarized Morphogenesis Regulator Spa2 Is Required for the Function of Putative Stretch-Activated Ca2+-Permeable Channel Component Mid1 in Saccharomyces cerevisiae Eukaryot. Cell, August 1, 2005; 4(8): 1353 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-F. Wang, L.-M. Fan, W.-Z. Zhang, W. Zhang, and W.-H. Wu Ca2+-Permeable Channels in the Plasma Membrane of Arabidopsis Pollen Are Regulated by Actin Microfilaments Plant Physiology, December 1, 2004; 136(4): 3892 - 3904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sze, S. Padmanaban, F. Cellier, D. Honys, N.-H. Cheng, K. W. Bock, G. Conejero, X. Li, D. Twell, J. M. Ward, et al. Expression Patterns of a Novel AtCHX Gene Family Highlight Potential Roles in Osmotic Adjustment and K+ Homeostasis in Pollen Development Plant Physiology, September 1, 2004; 136(1): 2532 - 2547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Dutta and K. R. Robinson Identification and Characterization of Stretch-Activated Ion Channels in Pollen Protoplasts Plant Physiology, July 1, 2004; 135(3): 1398 - 1406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sampson, R. R. Lew, and I. B. Heath Time series analysis demonstrates the absence of pulsatile hyphal growth Microbiology, November 1, 2003; 149(11): 3111 - 3119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Silverman-Gavrila and R. R. Lew Calcium gradient dependence of Neurospora crassa hyphal growth Microbiology, September 1, 2003; 149(9): 2475 - 2485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zonia and J. A. Feijo State and Spectral Properties of Chloride Oscillations in Pollen Biophys. J., February 1, 2003; 84(2): 1387 - 1398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Robinson and M. A. Messerli Pulsating Ion Fluxes and Growth at the Pollen Tube Tip Sci. Signal., December 10, 2002; 2002(162): pe51 - pe51. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zonia, S. Cordeiro, J. Tupy, and J. A. Feijo Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by Inositol 3,4,5,6-Tetrakisphosphate PLANT CELL, September 1, 2002; 14(9): 2233 - 2249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Mouline, A.-A. Very, F. Gaymard, J. Boucherez, G. Pilot, M. Devic, D. Bouchez, J.-B. Thibaud, and H. Sentenac Pollen tube development and competitive ability are impaired by disruption of a Shaker K+ channel in Arabidopsis Genes & Dev., February 1, 2002; 16(3): 339 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Allen and J. I. Schroeder Combining Genetics and Cell Biology to Crack the Code of Plant Cell Calcium Signaling Sci. Signal., October 2, 2001; 2001(102): re13 - re13. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-M. Fan, Y.-F. Wang, H. Wang, and W.-H. Wu In vitro Arabidopsis pollen germination and characterization of the inward potassium currents in Arabidopsis pollen grain protoplasts J. Exp. Bot., August 1, 2001; 52(361): 1603 - 1614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Safadi, V. S. Reddy, and A. S. N. Reddy A Pollen-specific Novel Calmodulin-binding Protein with Tetratricopeptide Repeats J. Biol. Chem., November 3, 2000; 275(45): 35457 - 35470. [Abstract] [Full Text] [PDF]
|
|
