|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 10 1621-1632, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
MT Nieman, JB Kim, KR Johnson and MJ Wheelock
Department of Biology, University of Toledo, Toledo, OH 43606, USA.
The cadherin/catenin complex mediates Ca2+-dependent cell-cell interactions that are essential for normal developmental processes. It has been proposed that sorting of cells during embryonic development is due, at least in part, to expression of different cadherin family members or to expression of differing levels of a single family member. Expression of dominant-negative cadherins has been used experimentally to decrease cell-cell interactions in whole organisms and in cultured cells. In this study, we elucidated the mechanism of action of extracellular domain-deleted dominant-negative cadherin, showing that it is not cadherin isotype-specific, and that it must be membrane-associated but the orientation within the membrane does not matter. In addition, membrane-targeted cytoplasmic domain cadherin with the catenin-binding domain deleted does not function as a dominant-negative cadherin. Expression of extracellular domain-deleted dominant-negative cadherin results in down-regulation of endogenous cadherins which presumably contributes to the non-adhesive phenotype.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  M. U. Naik, T. U. Naik, A. T. Suckow, M. K. Duncan, and U. P. Naik Attenuation of Junctional Adhesion Molecule-A Is a Contributing Factor for Breast Cancer Cell Invasion Cancer Res., April 1, 2008; 68(7): 2194 - 2203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Andl, B. B. Fargnoli, T. Okawa, M. Bowser, M. Takaoka, H. Nakagawa, A. Klein-Szanto, X. Hua, M. Herlyn, and A. K. Rustgi Coordinated Functions of E-Cadherin and Transforming Growth Factor {beta} Receptor II In vitro and In vivo. Cancer Res., October 15, 2006; 66(20): 9878 - 9885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Orrington-Myers, X. Gao, P. Kouklis, M. Broman, A. Rahman, S. M. Vogel, and A. B. Malik Regulation of lung neutrophil recruitment by VE-cadherin Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L764 - L771. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Andersen, J. Mejlvang, S. Mahmood, I. Gromova, P. Gromov, E. Lukanidin, M. Kriajevska, J. K. Mellon, and E. Tulchinsky Immediate and Delayed Effects of E-Cadherin Inhibition on Gene Regulation and Cell Motility in Human Epidermoid Carcinoma Cells Mol. Cell. Biol., October 15, 2005; 25(20): 9138 - 9150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Johnson, C. S. Theisen, K. R. Johnson, and M. J. Wheelock R-cadherin Influences Cell Motility via Rho Family GTPases J. Biol. Chem., July 23, 2004; 279(30): 31041 - 31049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. H. M. Castro, C. S. Shin, J. P. Stains, S.-L. Cheng, S. Sheikh, G. Mbalaviele, V. L. Szejnfeld, and R. Civitelli Targeted expression of a dominant-negative N-cadherin in vivo delays peak bone mass and increases adipogenesis J. Cell Sci., June 1, 2004; 117(13): 2853 - 2864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Iyer, D. M. Ferreri, N. C. DeCocco, F. L. Minnear, and P. A. Vincent VE-cadherin-p120 interaction is required for maintenance of endothelial barrier function Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1143 - L1153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Vincent, K. Xiao, K. M. Buckley, and A. P. Kowalczyk VE-cadherin: adhesion at arm's length Am J Physiol Cell Physiol, May 1, 2004; 286(5): C987 - C997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Guo, M. H. Wu, H. J. Granger, and S. Y. Yuan Transference of recombinant VE-cadherin cytoplasmic domain alters endothelial junctional integrity and porcine microvascular permeability J. Physiol., January 1, 2004; 554(1): 78 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. B. Uglow, S. Slater, G. B. Sala-Newby, C. M. Aguilera-Garcia, G. D. Angelini, A. C. Newby, and S. J. George Dismantling of Cadherin-Mediated Cell-Cell Contacts Modulates Smooth Muscle Cell Proliferation Circ. Res., June 27, 2003; 92(12): 1314 - 1321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Xiao, D. F. Allison, M. D. Kottke, S. Summers, G. P. Sorescu, V. Faundez, and A. P. Kowalczyk Mechanisms of VE-cadherin Processing and Degradation in Microvascular Endothelial Cells J. Biol. Chem., May 23, 2003; 278(21): 19199 - 19208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kouklis, M. Konstantoulaki, and A. B. Malik VE-cadherin-induced Cdc42 Signaling Regulates Formation of Membrane Protrusions in Endothelial Cells J. Biol. Chem., April 25, 2003; 278(18): 16230 - 16236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tanaka, H. Nakanishi, S. Kakunaga, N. Okabe, T. Kawakatsu, K. Shimizu, and Y. Takai Role of Nectin in Formation of E-Cadherin-based Adherens Junctions in Keratinocytes: Analysis with the N-Cadherin Dominant Negative Mutant Mol. Biol. Cell, April 1, 2003; 14(4): 1597 - 1609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rios-Doria, K. C. Day, R. Kuefer, M. G. Rashid, A. M. Chinnaiyan, M. A. Rubin, and M. L. Day The Role of Calpain in the Proteolytic Cleavage of E-cadherin in Prostate and Mammary Epithelial Cells J. Biol. Chem., January 3, 2003; 278(2): 1372 - 1379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Klingelhofer, O. Y. Laur, R. B. Troyanovsky, and S. M. Troyanovsky Dynamic Interplay between Adhesive and Lateral E-Cadherin Dimers Mol. Cell. Biol., November 1, 2002; 22(21): 7449 - 7458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Venkiteswaran, K. Xiao, S. Summers, C. C. Calkins, P. A. Vincent, K. Pumiglia, and A. P. Kowalczyk Regulation of endothelial barrier function and growth by VE-cadherin, plakoglobin, and beta -catenin Am J Physiol Cell Physiol, September 1, 2002; 283(3): C811 - C821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ozawa Lateral Dimerization of the E-cadherin Extracellular Domain Is Necessary but Not Sufficient for Adhesive Activity J. Biol. Chem., May 24, 2002; 277(22): 19600 - 19608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V Noe, B Fingleton, K Jacobs, H. Crawford, S Vermeulen, W Steelant, E Bruyneel, L. Matrisian, and M Mareel Release of an invasion promoter E-cadherin fragment by matrilysin and stromelysin-1 J. Cell Sci., January 1, 2001; 114(1): 111 - 118. [Abstract] [PDF]
|
|
|
|
 |
|
 J.-B. Kim, S. Islam, Y. J. Kim, R. S. Prudoff, K. M. Sass, M. J. Wheelock, and K. R. Johnson N-Cadherin Extracellular Repeat 4 Mediates Epithelial to Mesenchymal Transition and Increased Motility J. Cell Biol., December 11, 2000; 151(6): 1193 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Vallorosi, K. C. Day, X. Zhao, M. G. Rashid, M. A. Rubin, K. R. Johnson, M. J. Wheelock, and M. L. Day Truncation of the beta -Catenin Binding Domain of E-cadherin Precedes Epithelial Apoptosis during Prostate and Mammary Involution J. Biol. Chem., February 4, 2000; 275(5): 3328 - 3334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y Hanakawa, M Amagai, Y Shirakata, K Sayama, and K Hashimoto Different effects of dominant negative mutants of desmocollin and desmoglein on the cell-cell adhesion of keratinocytes J. Cell Sci., January 5, 2000; 113(10): 1803 - 1811. [Abstract] [PDF]
|
|
|
|
|
|
P. Anastasiadis and A. Reynolds The p120 catenin family: complex roles in adhesion, signaling and cancer J. Cell Sci., January 4, 2000; 113(8): 1319 - 1334. [Abstract] [PDF]
|
|
|
|
|
|
M. Troxell, S Gopalakrishnan, J McCormack, B. Poteat, J Pennington, S. Garringer, E. Schneeberger, W. Nelson, and J. Marrs Inhibiting cadherin function by dominant mutant E-cadherin expression increases the extent of tight junction assembly J. Cell Sci., January 3, 2000; 113(6): 985 - 996. [Abstract] [PDF]
|
|
|
|
|
|
O Huber, R Kemler, and D Langosch Mutations affecting transmembrane segment interactions impair adhesiveness of E-cadherin J. Cell Sci., January 12, 1999; 112(23): 4415 - 4423. [Abstract] [PDF]
|
|
