|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 11 1709-1719, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
C Ostlund, J Ellenberg, E Hallberg, J Lippincott-Schwartz and HJ Worman
Department of Medicine and of Anatomy and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Emerin is an integral protein of the inner nuclear membrane that is mutated or not expressed in patients with Emery-Dreifuss muscular dystrophy. Confocal immunofluorescence microscopy studies of the intracellular targeting of truncated forms of emerin, some of which are found in patients with Emery-Dreifuss muscular dystrophy, show that the nucleoplasmic, amino-terminal domain is necessary and sufficient for nuclear retention. When this domain is fused to a transmembrane segment of an integral membrane protein of the ER/plasma membrane, the chimeric protein is localized in the inner nuclear membrane. The transmembrane segment of emerin is not targeted to the inner nuclear membrane. Fluorescence photobleaching experiments of emerin fused to green fluorescent protein demonstrate that the diffusional mobility (D) of emerin is decreased in the inner nuclear membrane (D=0.10+/-0.01 microm2/second) compared to the ER membrane (D=0.32+/-0.01 microm2/second). This is in agreement with a model where integral proteins reach the inner nuclear membrane by lateral diffusion and are retained there by association with nucleoplasmic components. Some overexpressed emerin-green fluorescent protein also reaches the plasma membrane of transfected cells, where its diffusion is similar to that in the inner nuclear membrane, suggesting that emerin may also associate with non-nuclear structures.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Haque, D. Mazzeo, J. T. Patel, D. T. Smallwood, J. A. Ellis, C. M. Shanahan, and S. Shackleton Mammalian SUN Protein Interaction Networks at the Inner Nuclear Membrane and Their Role in Laminopathy Disease Processes J. Biol. Chem., January 29, 2010; 285(5): 3487 - 3498. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ostlund, E. S. Folker, J. C. Choi, E. R. Gomes, G. G. Gundersen, and H. J. Worman Dynamics and molecular interactions of linker of nucleoskeleton and cytoskeleton (LINC) complex proteins J. Cell Sci., November 15, 2009; 122(22): 4099 - 4108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lu, M. S. Ladinsky, and T. Kirchhausen Cisternal Organization of the Endoplasmic Reticulum during Mitosis Mol. Biol. Cell, August 1, 2009; 20(15): 3471 - 3480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, A. A. Panteleyev, D. M. Owens, K. Djabali, C. L. Stewart, and H. J. Worman Epidermal expression of the truncated prelamin A causing Hutchinson-Gilford progeria syndrome: effects on keratinocytes, hair and skin Hum. Mol. Genet., August 1, 2008; 17(15): 2357 - 2369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Braunagel, S. T. Williamson, Q. Ding, X. Wu, and M. D. Summers Early sorting of inner nuclear membrane proteins is conserved PNAS, May 29, 2007; 104(22): 9307 - 9312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Morris, H. Hofemeister, and P. O'Hare Herpes Simplex Virus Infection Induces Phosphorylation and Delocalization of Emerin, a Key Inner Nuclear Membrane Protein J. Virol., May 1, 2007; 81(9): 4429 - 4437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. S. Wilkie and E. C. Schirmer Guilt by Association: The Nuclear Envelope Proteome and Disease Mol. Cell. Proteomics, October 1, 2006; 5(10): 1865 - 1875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, A. J. Herron, and H. J. Worman Pathology and nuclear abnormalities in hearts of transgenic mice expressing M371K lamin A encoded by an LMNA mutation causing Emery-Dreifuss muscular dystrophy Hum. Mol. Genet., August 15, 2006; 15(16): 2479 - 2489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Haque, D. J. Lloyd, D. T. Smallwood, C. L. Dent, C. M. Shanahan, A. M. Fry, R. C. Trembath, and S. Shackleton SUN1 Interacts with Nuclear Lamin A and Cytoplasmic Nesprins To Provide a Physical Connection between the Nuclear Lamina and the Cytoskeleton Mol. Cell. Biol., May 15, 2006; 26(10): 3738 - 3751. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ozawa, Y. K. Hayashi, M. Ogawa, R. Kurokawa, H. Matsumoto, S. Noguchi, I. Nonaka, and I. Nishino Emerin-Lacking Mice Show Minimal Motor and Cardiac Dysfunctions with Nuclear-Associated Vacuoles Am. J. Pathol., March 1, 2006; 168(3): 907 - 917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Boguslavsky, C. L. Stewart, and H. J. Worman Nuclear lamin A inhibits adipocyte differentiation: implications for Dunnigan-type familial partial lipodystrophy Hum. Mol. Genet., February 15, 2006; 15(4): 653 - 663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Libotte, H. Zaim, S. Abraham, V. C. Padmakumar, M. Schneider, W. Lu, M. Munck, C. Hutchison, M. Wehnert, B. Fahrenkrog, et al. Lamin A/C-dependent Localization of Nesprin-2, a Giant Scaffolder at the Nuclear Envelope Mol. Biol. Cell, July 1, 2005; 16(7): 3411 - 3424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liang and J. D. Baines Identification of an Essential Domain in the Herpes Simplex Virus 1 UL34 Protein That Is Necessary and Sufficient To Interact with UL31 Protein J. Virol., March 15, 2005; 79(6): 3797 - 3806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Goodchild and W. T. Dauer The AAA+ protein torsinA interacts with a conserved domain present in LAP1 and a novel ER protein J. Cell Biol., March 14, 2005; 168(6): 855 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ferreri-Jacobia, D.-O. D. Mak, and J. K. Foskett Translational Mobility of the Type 3 Inositol 1,4,5-Trisphosphate Receptor Ca2+ Release Channel in Endoplasmic Reticulum Membrane J. Biol. Chem., February 4, 2005; 280(5): 3824 - 3831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ohba, E. C. Schirmer, T. Nishimoto, and L. Gerace Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore J. Cell Biol., December 20, 2004; 167(6): 1051 - 1062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Dechat, A. Gajewski, B. Korbei, D. Gerlich, N. Daigle, T. Haraguchi, K. Furukawa, J. Ellenberg, and R. Foisner LAP2{alpha} and BAF transiently localize to telomeres and specific regions on chromatin during nuclear assembly J. Cell Sci., December 1, 2004; 117(25): 6117 - 6128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Sorensen, A. Brech, D. Khnykin, E. Kolpakova, L. Citores, and S. Olsnes Deletion mutant of FGFR4 induces onion-like membrane structures in the nucleus J. Cell Sci., May 1, 2004; 117(9): 1807 - 1819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Zastrow, S. Vlcek, and K. L. Wilson Proteins that bind A-type lamins: integrating isolated clues J. Cell Sci., March 1, 2004; 117(7): 979 - 987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Salina, P. Enarson, J.B. Rattner, and B. Burke Nup358 integrates nuclear envelope breakdown with kinetochore assembly J. Cell Biol., September 15, 2003; 162(6): 991 - 1001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Holt, C. Ostlund, C. L. Stewart, N. t. Man, H. J. Worman, and G. E. Morris Effect of pathogenic mis-sense mutations in lamin A on its interaction with emerin in vivo J. Cell Sci., July 15, 2003; 116(14): 3027 - 3035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Rolls, D. H. Hall, M. Victor, E. H. K. Stelzer, and T. A. Rapoport Targeting of Rough Endoplasmic Reticulum Membrane Proteins and Ribosomes in Invertebrate Neurons Mol. Biol. Cell, May 1, 2002; 13(5): 1778 - 1791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ostlund, G. Bonne, K. Schwartz, and H. J. Worman Properties of lamin A mutants found in Emery-Dreifuss muscular dystrophy, cardiomyopathy and Dunnigan-type partial lipodystrophy J. Cell Sci., March 14, 2002; 114(24): 4435 - 4445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. H. Raharjo, P. Enarson, T. Sullivan, C. L. Stewart, and B. Burke Nuclear envelope defects associated with LMNA mutations cause dilated cardiomyopathy and Emery-Dreifuss muscular dystrophy J. Cell Sci., March 14, 2002; 114(24): 4447 - 4457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Lee, T. Haraguchi, R. S. Lee, T. Koujin, Y. Hiraoka, and K. L. Wilson Distinct functional domains in emerin bind lamin A and DNA-bridging protein BAF J. Cell Sci., March 14, 2002; 114(24): 4567 - 4573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Haraguchi, T. Koujin, M. Segura-Totten, K. K. Lee, Y. Matsuoka, Y. Yoneda, K. L. Wilson, and Y. Hiraoka BAF is required for emerin assembly into the reforming nuclear envelope J. Cell Sci., March 14, 2002; 114(24): 4575 - 4585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Bh. Muralikrishna, J. Dhawan, N. Rangaraj, and V. K. Parnaik Distinct changes in intranuclear lamin A/C organization during myoblast differentiation J. Cell Sci., March 13, 2002; 114(22): 4001 - 4011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. Vaughan, M. Alvarez-Reyes, J. M. Bridger, J. L. V. Broers, F. C. S. Ramaekers, M. Wehnert, G. E. Morris, W. G. F. Whitfield, and C. J. Hutchison Both emerin and lamin C depend on lamin A for localization at the nuclear envelope J. Cell Sci., March 9, 2002; 114(14): 2577 - 2590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. H. C. Lam, R. J. Thomas, K. L. Loveland, S. Schilders, M. Gu, T. J. Martin, M. T. Gillespie, and D. A. Jans Nuclear Transport of Parathyroid Hormone (PTH)-Related Protein Is Dependent on Microtubules Mol. Endocrinol., February 1, 2002; 16(2): 390 - 401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. L. Fairley, A. Riddell, J. A. Ellis, and J. Kendrick-Jones The cell cycle dependent mislocalisation of emerin may contribute to the Emery-Dreifuss muscular dystrophy phenotype J. Cell Sci., January 15, 2002; 115(2): 341 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wu, F. Lin, and H. J. Worman Intracellular trafficking of MAN1, an integral protein of the nuclear envelope inner membrane J. Cell Sci., January 4, 2002; 115(7): 1361 - 1371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Gruenbaum, K. K. Lee, J. Liu, M. Cohen, and K. L. Wilson The expression, lamin-dependent localization and RNAi depletion phenotype for emerin in C. elegans J. Cell Sci., January 3, 2002; 115(5): 923 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Reynolds, B. J. Ryckman, J. D. Baines, Y. Zhou, L. Liang, and R. J. Roller UL31 and UL34 Proteins of Herpes Simplex Virus Type 1 Form a Complex That Accumulates at the Nuclear Rim and Is Required for Envelopment of Nucleocapsids J. Virol., September 15, 2001; 75(18): 8803 - 8817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Scott and P. O'Hare Fate of the Inner Nuclear Membrane Protein Lamin B Receptor and Nuclear Lamins in Herpes Simplex Virus Type 1 Infection J. Virol., September 15, 2001; 75(18): 8818 - 8830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Daigle, J. Beaudouin, L. Hartnell, G. Imreh, E. Hallberg, J. Lippincott-Schwartz, and J. Ellenberg Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells J. Cell Biol., July 9, 2001; 154(1): 71 - 84. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Tolonen, L. Doglio, S. Schleich, and J. K. Locker Vaccinia Virus DNA Replication Occurs in Endoplasmic Reticulum-enclosed Cytoplasmic Mini-Nuclei Mol. Biol. Cell, July 1, 2001; 12(7): 2031 - 2046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Boyle, S. Gilchrist, J. M. Bridger, N. L. Mahy, J. A. Ellis, and W. A. Bickmore The spatial organization of human chromosomes within the nuclei of normal and emerin-mutant cells Hum. Mol. Genet., February 1, 2001; 10(3): 211 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Lee, Y. Gruenbaum, P. Spann, J. Liu, and K. L. Wilson C. elegans Nuclear Envelope Proteins Emerin, MAN1, Lamin, and Nucleoporins Reveal Unique Timing of Nuclear Envelope Breakdown during Mitosis Mol. Biol. Cell, September 1, 2000; 11(9): 3089 - 3099. [Abstract] [Full Text]
|
|
|
|
|
|
F. Lin, D. L. Blake, I. Callebaut, I. S. Skerjanc, L. Holmer, M. W. McBurney, M. Paulin-Levasseur, and H. J. Worman MAN1, an Inner Nuclear Membrane Protein That Shares the LEM Domain with Lamina-associated Polypeptide 2 and Emerin J. Biol. Chem., February 18, 2000; 275(7): 4840 - 4847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Haraguchi, T Koujin, T Hayakawa, T Kaneda, C Tsutsumi, N Imamoto, C Akazawa, J Sukegawa, Y Yoneda, and Y Hiraoka Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes J. Cell Sci., January 3, 2000; 113(5): 779 - 794. [Abstract] [PDF]
|
|
|
|
|
|
T. Sullivan, D. Escalante-Alcalde, H. Bhatt, M. Anver, N. Bhat, K. Nagashima, C. L. Stewart, and B. Burke Loss of a-Type Lamin Expression Compromises Nuclear Envelope Integrity Leading to Muscular Dystrophy J. Cell Biol., November 29, 1999; 147(5): 913 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Rolls, P. A. Stein, S. S. Taylor, E. Ha, F. McKeon, and T. A. Rapoport A Visual Screen of a Gfp-Fusion Library Identifies a New Type of Nuclear Envelope Membrane Protein J. Cell Biol., July 12, 1999; 146(1): 29 - 44. [Abstract] [Full Text] [PDF]
|
|
