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Journal of Cell Science, Vol 112, Issue 12 1889-1900, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
TM Savino, R Bastos, E Jansen and D Hernandez-Verdun
Nuclei and Cell Cycle, Institut Jacques Monod, UMR 7592, Paris, France.
We report the molecular characterization of a novel nucleolar protein, Nop52, and its subcellular distribution during the cell cycle and nucleologenesis. This protein was originally identified with human autoantibodies which were subsequently used to clone its corresponding cDNA. Transfection experiments in mammalian cells have confirmed that this cDNA encodes a nucleolar protein that accumulates in the nucleoli and at the periphery of the chromosomes. Nop52 is the putative human homologue of the yeast ribosomal RNA processing protein RRP1 which is involved in pre-rRNA processing from 27S to 25S and 5.8S. In nucleoli, Nop52 is excluded from the ribosomal RNA transcription sites, accumulates in the granular external domain and mainly colocalizes with nucleolar proteins involved in the late processing step such as hPop1 and protein B23. During the building process of the nucleolus at the end of mitosis, a sequential order was observed in the assembly of nucleolar proteins of early and late processing mainly via the prenucleolar body pathway. The order is the following: fibrillarin, nucleolin, Nop52 together with protein B23 in the prenucleolar bodies, and simultaneously with hPop1, and finally Ki-67. The evolutionary conservation of Nop52 and the lethal effects observed in gene disruption experiments, predict a critical role for Nop52 in the generation of 28S rRNA.
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 T. Lechertier, V. Sirri, D. Hernandez-Verdun, and P. Roussel A B23-interacting sequence as a tool to visualize protein interactions in a cellular context J. Cell Sci., January 15, 2007; 120(2): 265 - 275. [Abstract] [Full Text] [PDF]
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E. Louvet, H. R. Junera, I. Berthuy, and D. Hernandez-Verdun Compartmentation of the Nucleolar Processing Proteins in the Granular Component Is a CK2-driven Process Mol. Biol. Cell, June 1, 2006; 17(6): 2537 - 2546. [Abstract] [Full Text] [PDF]
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N. Angelier, M. Tramier, E. Louvet, M. Coppey-Moisan, T. M. Savino, J. R. De Mey, and D. Hernandez-Verdun Tracking the Interactions of rRNA Processing Proteins during Nucleolar Assembly in Living Cells Mol. Biol. Cell, June 1, 2005; 16(6): 2862 - 2871. [Abstract] [Full Text] [PDF]
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 E. W. HORSEY, J. JAKOVLJEVIC, T. D. MILES, P. HARNPICHARNCHAI, and J. L. WOOLFORD JR. Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation RNA, May 1, 2004; 10(5): 813 - 827. [Abstract] [Full Text] [PDF]
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 V. Sirri, D. Hernandez-Verdun, and P. Roussel Cyclin-dependent kinases govern formation and maintenance of the nucleolus J. Cell Biol., March 18, 2002; 156(6): 969 - 981. [Abstract] [Full Text] [PDF]
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D. Hernandez-Verdun, P. Roussel, and J. Gebrane-Younes Emerging concepts of nucleolar assembly J. Cell Sci., January 6, 2002; 115(11): 2265 - 2270. [Abstract] [Full Text] [PDF]
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T. M. Savino, J. Gebrane-Younes, J. De Mey, J.-B. Sibarita, and D. Hernandez-Verdun Nucleolar Assembly of the rRNA Processing Machinery in Living Cells J. Cell Biol., May 29, 2001; 153(5): 1097 - 1110. [Abstract] [Full Text] [PDF]
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M. Dundr, T. Misteli, and M. O.J. Olson The Dynamics of Postmitotic Reassembly of the Nucleolus J. Cell Biol., August 7, 2000; 150(3): 433 - 446. [Abstract] [Full Text] [PDF]
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T. Dousset, C. Wang, C. Verheggen, D. Chen, D. Hernandez-Verdun, and S. Huang Initiation of Nucleolar Assembly Is Independent of RNA Polymerase I Transcription Mol. Biol. Cell, August 1, 2000; 11(8): 2705 - 2717. [Abstract] [Full Text]
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C. Verheggen, G. Almouzni, and D. Hernandez-Verdun The Ribosomal RNA Processing Machinery Is Recruited to the Nucleolar Domain before RNA Polymerase I during Xenopus laevis Development J. Cell Biol., April 17, 2000; 149(2): 293 - 306. [Abstract] [Full Text] [PDF]
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Y. Yang, C. Isaac, C. Wang, F. Dragon, and U. T. Meier Conserved Composition of Mammalian Box H/ACA and Box C/D Small Nucleolar Ribonucleoprotein Particles and Their Interaction with the Common Factor Nopp140 Mol. Biol. Cell, February 1, 2000; 11(2): 567 - 577. [Abstract] [Full Text]
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V. Sirri, P. Roussel, and D. Hernandez-Verdun In Vivo Release of Mitotic Silencing of Ribosomal Gene Transcription Does Not Give Rise to Precursor Ribosomal RNA Processing J. Cell Biol., January 24, 2000; 148(2): 259 - 270. [Abstract] [Full Text] [PDF]
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