|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 12 1915-1923, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
PL Hordijk, E Anthony, FP Mul, R Rientsma, LC Oomen and D Roos
Dept of Experimental Immunohematology, CLB and Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam. hordijk@clb.nl
Vascular endothelial (VE)-cadherin is the endothelium-specific member of the cadherin family of homotypic cell adhesion molecules. VE-cadherin, but not the cell adhesion molecule platelet/endothelial cell adhesion molecule (PECAM-1), markedly colocalizes with actin stress fibers at cell-cell junctions between human umbilical vein endothelial cells. Inhibition of VE-cadherin-mediated, but not PECAM-1-mediated, adhesion induced reorganization of the actin cytoskeleton, loss of junctional VE-cadherin staining and loss of cell-cell adhesion. In functional assays, inhibition of VE-cadherin caused increased monolayer permeability and enhanced neutrophil transendothelial migration. In a complementary set of experiments, modulation of the actin cytoskeleton was found to strongly affect VE-cadherin distribution. Brief stimulation of the beta2-adrenergic receptor with isoproterenol induced a loss of actin stress fibers resulting in a linear, rather than 'jagged', VE-cadherin distribution. The concomitant, isoproterenol-induced, reduction in monolayer permeability was alleviated by a VE-cadherin-blocking antibody. Finally, cytoskeletal reorganization resulting from the inactivation of p21Rho caused a diffuse localization of VE-cadherin, which was accompanied by reduced cell-cell adhesion. Together, these data show that monolayer permeability and neutrophil transendothelial migration are modulated by VE-cadherin-mediated cell-cell adhesion, which is in turn controlled by the dynamics of the actin cytoskeleton.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Regan-Klapisz, V. Krouwer, M. Langelaar-Makkinje, L. Nallan, M. Gelb, H. Gerritsen, A. J. Verkleij, and J. A. Post Golgi-associated cPLA2{alpha} Regulates Endothelial Cell-Cell Junction Integrity by Controlling the Trafficking of Transmembrane Junction Proteins Mol. Biol. Cell, October 1, 2009; 20(19): 4225 - 4234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Monaghan-Benson and K. Burridge The Regulation of Vascular Endothelial Growth Factor-induced Microvascular Permeability Requires Rac and Reactive Oxygen Species J. Biol. Chem., September 18, 2009; 284(38): 25602 - 25611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-M. Heupel, A. Efthymiadis, N. Schlegel, T. Muller, Y. Baumer, W. Baumgartner, D. Drenckhahn, and J. Waschke Endothelial barrier stabilization by a cyclic tandem peptide targeting VE-cadherin transinteraction in vitro and in vivo J. Cell Sci., May 15, 2009; 122(10): 1616 - 1625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Tonino, R. Spijker, D. M.P. Luijks, M. H. J. van Oers, and A. P. Kater No convincing evidence for a role of CD31-CD38 interactions in the pathogenesis of chronic lymphocytic leukemia Blood, August 1, 2008; 112(3): 840 - 843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bibert, H. Ayari, D. Riveline, E. Concord, B. Hermant, T. Vernet, and D. Gulino-Debrac Establishment of cell-cell junctions depends on the oligomeric states of VE-cadherin J. Biochem., June 1, 2008; 143(6): 821 - 832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. E. Dewi, T. Takasaki, and I. Kurane Peripheral blood mononuclear cells increase the permeability of dengue virus-infected endothelial cells in association with downregulation of vascular endothelial cadherin J. Gen. Virol., March 1, 2008; 89(3): 642 - 652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Muller-Marschhausen, J. Waschke, and D. Drenckhahn Physiological hydrostatic pressure protects endothelial monolayer integrity Am J Physiol Cell Physiol, January 1, 2008; 294(1): C324 - C332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Furman, A. L. Sieminski, A. V. Kwiatkowski, D. A. Rubinson, E. Vasile, R. T. Bronson, R. Fassler, and F. B. Gertler Ena/VASP is required for endothelial barrier function in vivo J. Cell Biol., November 19, 2007; 179(4): 761 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Baluk, J. Fuxe, H. Hashizume, T. Romano, E. Lashnits, S. Butz, D. Vestweber, M. Corada, C. Molendini, E. Dejana, et al. Functionally specialized junctions between endothelial cells of lymphatic vessels J. Exp. Med., October 1, 2007; 204(10): 2349 - 2362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Bentz, M. Jarquin-Pardo, G. Chan, M. S. Smith, C. Sinzger, and A. D. Yurochko Human Cytomegalovirus (HCMV) Infection of Endothelial Cells Promotes Naive Monocyte Extravasation and Transfer of Productive Virus To Enhance Hematogenous Dissemination of HCMV J. Virol., December 1, 2006; 80(23): 11539 - 11555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Orrington-Myers, X. Gao, P. Kouklis, M. Broman, A. Rahman, S. M. Vogel, and A. B. Malik Regulation of lung neutrophil recruitment by VE-cadherin Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L764 - L771. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. van Buul, E. C. Anthony, M. Fernandez-Borja, K. Burridge, and P. L. Hordijk Proline-rich Tyrosine Kinase 2 (Pyk2) Mediates Vascular Endothelial-Cadherin-based Cell-Cell Adhesion by Regulating {beta}-Catenin Tyrosine Phosphorylation J. Biol. Chem., June 3, 2005; 280(22): 21129 - 21136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Warfel, A. D. Steele, and F. D'Agnillo Anthrax Lethal Toxin Induces Endothelial Barrier Dysfunction Am. J. Pathol., June 1, 2005; 166(6): 1871 - 1881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Cook-Mills and T. L. Deem Active participation of endothelial cells in inflammation J. Leukoc. Biol., April 1, 2005; 77(4): 487 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Lambeng, Y. Wallez, C. Rampon, F. Cand, G. Christe, D. Gulino-Debrac, I. Vilgrain, and P. Huber Vascular Endothelial-Cadherin Tyrosine Phosphorylation in Angiogenic and Quiescent Adult Tissues Circ. Res., February 18, 2005; 96(3): 384 - 391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu, D. C. Cara, J. Kaur, E. Raharjo, S. C. Mullaly, J. Jongstra-Bilen, J. Jongstra, and P. Kubes LSP1 is an endothelial gatekeeper of leukocyte transendothelial migration J. Exp. Med., February 7, 2005; 201(3): 409 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Guo, M. H. Wu, H. J. Granger, and S. Y. Yuan Transference of recombinant VE-cadherin cytoplasmic domain alters endothelial junctional integrity and porcine microvascular permeability J. Physiol., January 1, 2004; 554(1): 78 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sanchez, T. Estrada-Hernandez, J.-H. Paik, M.-T. Wu, K. Venkataraman, V. Brinkmann, K. Claffey, and T. Hla Phosphorylation and Action of the Immunomodulator FTY720 Inhibits Vascular Endothelial Cell Growth Factor-induced Vascular Permeability J. Biol. Chem., November 21, 2003; 278(47): 47281 - 47290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Scortegagna, M. A. Morris, Y. Oktay, M. Bennett, and J. A. Garcia The HIF family member EPAS1/HIF-2{alpha} is required for normal hematopoiesis in mice Blood, September 1, 2003; 102(5): 1634 - 1640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Alevriadou CAMs and Rho small GTPases: gatekeepers for leukocyte transendothelial migration. Focus on "VCAM-1-mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration" Am J Physiol Cell Physiol, August 1, 2003; 285(2): C250 - C252. [Full Text] [PDF]
|
|
|
|
|
|
S. van Wetering, N. van den Berk, J. D. van Buul, F. P. J. Mul, I. Lommerse, R. Mous, J.-P. t. Klooster, J.-J. Zwaginga, and P. L. Hordijk VCAM-1-mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration Am J Physiol Cell Physiol, August 1, 2003; 285(2): C343 - C352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Konstantoulaki, P. Kouklis, and A. B. Malik Protein kinase C modifications of VE-cadherin, p120, and {beta}-catenin contribute to endothelial barrier dysregulation induced by thrombin Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L434 - L442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Baumgartner, G. J. Schutz, J. Wiegand, N. Golenhofen, and D. Drenckhahn Cadherin function probed by laser tweezer and single molecule fluorescence in vascular endothelial cells J. Cell Sci., March 15, 2003; 116(6): 1001 - 1011. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Evans, D. I. Blyth, T. Wong, S. Sanjar, and M. R. West Decreased Distribution of Lung Epithelial Junction Proteins after Intratracheal Antigen or Lipopolysaccharide Challenge: Correlation with Neutrophil Influx and Levels of BALF sE-Cadherin Am. J. Respir. Cell Mol. Biol., October 1, 2002; 27(4): 446 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bibert, M. Jaquinod, E. Concord, C. Ebel, E. Hewat, C. Vanbelle, P. Legrand, M. Weidenhaupt, T. Vernet, and D. Gulino-Debrac Synergy between Extracellular Modules of Vascular Endothelial Cadherin Promotes Homotypic Hexameric Interactions J. Biol. Chem., April 5, 2002; 277(15): 12790 - 12801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ozaki, S. Kawashima, T. Hirase, T. Yamashita, M. Namiki, N. Inoue, K.-i. Hirata, and M. Yokoyama Overexpression of Endothelial Nitric Oxide Synthase in Endothelial Cells Is Protective against Ischemia-Reperfusion Injury in Mouse Skeletal Muscle Am. J. Pathol., April 1, 2002; 160(4): 1335 - 1344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. van Buul, C. Voermans, V. van den Berg, E. C. Anthony, F. P. J. Mul, S. van Wetering, C. E. van der Schoot, and P. L. Hordijk Migration of Human Hematopoietic Progenitor Cells Across Bone Marrow Endothelium Is Regulated by Vascular Endothelial Cadherin J. Immunol., January 15, 2002; 168(2): 588 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. van Wetering, J. D. van Buul, S. Quik, F. P. J. Mul, E. C. Anthony, J.-P. t. Klooster, J. G. Collard, and P. L. Hordijk Reactive oxygen species mediate Rac-induced loss of cell-cell adhesion in primary human endothelial cells J. Cell Sci., January 5, 2002; 115(9): 1837 - 1846. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Dudek and J. G. N. Garcia Cytoskeletal regulation of pulmonary vascular permeability J Appl Physiol, October 1, 2001; 91(4): 1487 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Shaw, P. S. Bamba, B. N. Perkins, and F. W. Luscinskas Real-Time Imaging of Vascular Endothelial-Cadherin During Leukocyte Transmigration Across Endothelium J. Immunol., August 15, 2001; 167(4): 2323 - 2330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lum and K. A. Roebuck Oxidant stress and endothelial cell dysfunction Am J Physiol Cell Physiol, April 1, 2001; 280(4): C719 - C741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nusrat, J. R. Turner, and J. L. Madara Molecular Physiology and Pathophysiology of Tight Junctions: IV. Regulation of tight junctions by extracellular stimuli: nutrients, cytokines, and immune cells Am J Physiol Gastrointest Liver Physiol, November 1, 2000; 279(5): G851 - G857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chandrasekaran, C.-Z. He, H. Al-Barazi, H. C. Krutzsch, M. L. Iruela-Arispe, and D. D. Roberts Cell Contact-dependent Activation of alpha 3beta 1 Integrin Modulates Endothelial Cell Responses to Thrombospondin-1 Mol. Biol. Cell, September 1, 2000; 11(9): 2885 - 2900. [Abstract] [Full Text]
|
|
|
|
|
|
S Orsulic and R Kemler Expression of Eph receptors and ephrins is differentially regulated by E-cadherin J. Cell Sci., January 5, 2000; 113(10): 1793 - 1802. [Abstract] [PDF]
|
|
|
|
|
|
C Johnson-Leger, M Aurrand-Lions, and B. Imhof The parting of the endothelium: miracle, or simply a junctional affair? J. Cell Sci., January 3, 2000; 113(6): 921 - 933. [Abstract] [PDF]
|
|
|
|
|
|
P. Legrand, S. Bibert, M. Jaquinod, C. Ebel, E. Hewat, F. Vincent, C. Vanbelle, E. Concord, T. Vernet, and D. Gulino Self-assembly of the Vascular Endothelial Cadherin Ectodomain in a Ca2+-dependent Hexameric Structure J. Biol. Chem., January 26, 2001; 276(5): 3581 - 3588. [Abstract] [Full Text] [PDF]
|
|
