spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shu, S.
Right arrow Articles by Uyeda, T. Q.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shu, S.
Right arrow Articles by Uyeda, T. Q.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 112, Issue 13 2195-2201, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

Role of myosin II tail sequences in its function and localization at the cleavage furrow in Dictyostelium

S Shu, RJ Lee, JM LeBlanc-Straceski and TQ Uyeda
Biomolecular Research Group, National Institute for Advanced Interdisciplinary Research, Tsukuba, Ibaraki 305-8562, Japan.

Cytoplasmic myosin II accumulates in the cleavage furrow and provides the force for cytokinesis in animal and amoeboid cells. One model proposes that a specific domain in the myosin II tail is responsible for its localization, possibly by interacting with a factor concentrated in the equatorial region. To test this possibility, we have expressed myosins carrying mutations in the tail domain in a strain of Dictyostelium cells from which the endogenous myosin heavy chain gene has been deleted. The mutations used in this study include four internal tail deletions: Mydelta824-941, Mydelta943-1464, Mydelta943-1194 and Mydelta1156-1464. Contrary to the prediction of the hypothesis, immunofluorescence staining demonstrated that all mutant myosins were able to move toward the furrow region. Chimeric myosins, which consisted of a Dictyostelium myosin head and chicken skeletal myosin tail, also efficiently localized to the cleavage furrow. All these deletion and chimeric mutant myosins, except for Mydelta943-1464, the largest deletion mutant, were able to support cytokinesis in suspension. Our data suggest that there is no single specific domain in the tail of Dictyostelium myosin II that is required for its functioning at and localization to the cleavage furrow.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
M. Lord, E. Laves, and T. D. Pollard
Cytokinesis Depends on the Motor Domains of Myosin-II in Fission Yeast but Not in Budding Yeast
Mol. Biol. Cell, November 1, 2005; 16(11): 5346 - 5355.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Shu, X. Liu, and E. D. Korn
Dictyostelium and Acanthamoeba myosin II assembly domains go to the cleavage furrow of Dictyostelium myosin II-null cells
PNAS, May 27, 2003; 100(11): 6499 - 6504.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Shu, X. Liu, C. A. Parent, T. Q. P. Uyeda, and E. D. Korn
Tail chimeras of Dictyostelium myosin II support cytokinesis and other myosin II activities but not full development
J. Cell Sci., November 15, 2002; 115(22): 4237 - 4249.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. P. Mulvihill, C. Barretto, and J. S. Hyams
Localization of Fission Yeast Type II Myosin, Myo2, to the Cytokinetic Actin Ring Is Regulated by Phosphorylation of a C-Terminal Coiled-Coil Domain and Requires a Functional Septation Initiation Network
Mol. Biol. Cell, December 1, 2001; 12(12): 4044 - 4053.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Yumura
Myosin II dynamics and cortical flow during contractile ring formation in Dictyostelium cells
J. Cell Biol., July 9, 2001; 154(1): 137 - 146.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
W. Liang, H. M. Warrick, and J. A. Spudich
A Structural Model for Phosphorylation Control of Dictyostelium Myosin II Thick Filament Assembly
J. Cell Biol., November 29, 1999; 147(5): 1039 - 1048.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1999