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Journal of Cell Science, Vol 112, Issue 14 2431-2440, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

Cell surface membrane homeostasis and intracellular membrane traffic balance in mouse L929 cells

GT Coupin, CD Muller, A Remy-Kristensen and JG Kuhry
Laboratoire d'Immunopharmacologie and Laboratoire de Pharmacologie et Physico-Chimie, UMR (ex ERS 653, ex URA491), Faculte de Pharmacie de Strasbourg, BP 24, France.

We have developed a simple method for synchronizing L929 mouse fibroblasts. Cultured as monolayers, these cells stop growing at confluency and arrest at the end of the G1 phase. Upon seeding at low density, they enter the S phase simultaneously. Using these cells we then looked at the evolution of the surface membrane area during the cell cycle using the fluorescence membrane probe TMA-DPH. In contact with cells, this probe partitions between the membrane (probe fluorescent) and the external medium (non-fluorescent), delivering a signal proportional to the membrane area. This area was constant until just before mitosis, when it increased at once. With the same probe as an endocytic marker, we examined how this membrane homeostasis could be consistent with intracellular membrane trafficking. The study was limited to one selected period of the cell cycle (6-9 hours). We observed that 14% of the membrane endocytosed was not recycled, but was replaced at the cell surface by newly formed membrane from biosynthetic pathways. Brefeldin A modified the membrane traffic, but not the overall membrane homeostasis. The results are discussed in the framework of a maturation model.


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© The Company of Biologists Ltd 1999