|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 17 2853-2865, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
V Tsakraklides, K Krogh, L Wang, JC Bizario, RE Larson, EM Espreafico and JS Wolenski
Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8103, USA.
Class-V myosins are two-headed actin-based mechanoenzymes that function in the transport and subcellular localization of organelles and possibly in the outgrowth of cellular processes. To determine which domains of myosin-V are involved in intracellular localization of this motor protein, we have expressed fusions of the green fluorescent protein with segments from two distinct myosin-V heavy chains. The expression patterns of constructs encoding four different domains of chick brain myosin-Va were compared to a single construct encoding the globular tail region of mouse myosin-Vb. In transfected mouse melanocytes, expression of the NH(2)-terminal head (catalytic domain) of chick brain myosin-Va codistributed with actin filaments throughout the cytoplasm. A similar construct encoding the myosin-Va head with the associated neck (light chain binding sites), also codistributed with actin filaments. The GFP-head-neck peptide was also highly concentrated in the tips of filopodia in B16 melanocytes wild type for myosin-Va (MYO5a gene), but was concentrated throughout the entire filopodia of S91-6 melanocytes derived from dilute mice with mutations in the MYO5a gene. Evidence is also presented that the globular tail of myosin-Va, but not myosin-Vb, targets this motor molecule to the centrosome as confirmed by colocalization in cells stained with antibodies to (gamma)-tubulin. Expression of the GFP-myosin-Va globular tail causes displacement of endogenous myosin-V from centrosomes as visualized by immunolabeling with antibodies to the head domain of myosin-V. Treatment with the microtubule-disrupting drug nocodazole markedly reduces myosin-V staining at the centrosome. In contrast, there was no detectable diminution of myosin-V staining at the centrosome in cells treated with the actin filament-disrupting drug cytochalasin D. Thus, while localization of the myosin-V motor domain to actin-rich regions is consistent with conventional models of actomyosin-based motility, localization to the centrosome occurs in the complete absence of the myosin-V motor domain and is dependent on intact microtubules.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Doyle, R. Martin-Garcia, A. T. Coulton, S. Bagley, and D. P. Mulvihill Fission yeast Myo51 is a meiotic spindle pole body component with discrete roles during cell fusion and spore formation J. Cell Sci., December 1, 2009; 122(23): 4330 - 4340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. You, T. Huang, E. J. Richer, J.-E. H. Schmidt, J. Zabner, Z. Borok, and S. L. Brody Role of f-box factor foxj1 in differentiation of ciliated airway epithelial cells Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L650 - L657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Petit, M.-L. Giron, J. Tobaly-Tapiero, P. Bittoun, E. Real, Y. Jacob, N. Tordo, H. de The, and A. Saib Targeting of incoming retroviral Gag to the centrosome involves a direct interaction with the dynein light chain 8 J. Cell Sci., August 15, 2003; 116(16): 3433 - 3442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. Kuroda, H. Ariga, and M. Fukuda The Actin-Binding Domain of Slac2-a/Melanophilin Is Required for Melanosome Distribution in Melanocytes Mol. Cell. Biol., August 1, 2003; 23(15): 5245 - 5255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. S. Bizario, F. A. Castro, J. F. Sousa, R. N. Fernandes, A. D. Damiao, M. K. Oliveira, P. V. B. Palma, R. E. Larson, J. C. Voltarelli, and E. M. Espreafico Myosin-V colocalizes with MHC class II in blood mononuclear cells and is up-regulated by T-lymphocyte activation J. Leukoc. Biol., February 1, 2002; 71(2): 195 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Scott, S. Leopardi, S. Printup, and B. C. Madden Filopodia are conduits for melanosome transfer to keratinocytes J. Cell Sci., January 4, 2002; 115(7): 1441 - 1451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. F. Tauhata, D. V. dos Santos, E. W. Taylor, M. S. Mooseker, and R. E. Larson High Affinity Binding of Brain Myosin-Va to F-actin Induced by Calcium in the Presence of ATP J. Biol. Chem., October 19, 2001; 276(43): 39812 - 39818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G. Patel, C. Liu, P. L. Cameron, and R. S. Cameron Myr 8, A Novel Unconventional Myosin Expressed during Brain Development Associates with the Protein Phosphatase Catalytic Subunits 1{alpha} and 1{gamma}1 J. Neurosci., October 15, 2001; 21(20): 7954 - 7968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Lapierre, R. Kumar, C. M. Hales, J. Navarre, S. G. Bhartur, J. O. Burnette, D. W. Provance Jr., J. A. Mercer, M. Bahler, and J. R. Goldenring Myosin Vb Is Associated with Plasma Membrane Recycling Systems Mol. Biol. Cell, June 1, 2001; 12(6): 1843 - 1857. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Hume, L. M. Collinson, A. Rapak, A. Q. Gomes, C. R. Hopkins, and M. C. Seabra Rab27a Regulates the Peripheral Distribution of Melanosomes in Melanocytes J. Cell Biol., February 19, 2001; 152(4): 795 - 808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Piel, P. Meyer, A. Khodjakov, C. L. Rieder, and M. Bornens The Respective Contributions of the Mother and Daughter Centrioles to Centrosome Activity and Behavior in Vertebrate Cells J. Cell Biol., April 17, 2000; 149(2): 317 - 330. [Full Text] [PDF]
|
|
