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Journal of Cell Science, Vol 112, Issue 17 2885-2893, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
WF Rothwell, CX Zhang, C Zelano, TS Hsieh and W Sullivan
Sinsheimer Laboratories, Department of Biology, University of California, Santa Cruz, CA 95064, USA.
During mitosis of the Drosophila cortical syncytial divisions, actin-based membrane furrows separate adjacent spindles. Our genetic analysis indicates that the centrosomal protein Nuf is specifically required for recruitment of components to the furrows and the membrane-associated protein Dah is primarily required for the inward invagination of the furrow membrane. Recruitment of actin, anillin and peanut to the furrows occurs normally in dah-derived embryos. However, subsequent invagination of the furrows fails in dah-derived embryos and the septins become dispersed throughout the cytoplasm. This indicates that stable septin localization requires Dah-mediated furrow invagination. Close examination of actin and Dah localization in wild-type embryos reveals that they associate in adjacent particles during interphase and co-localize in the invaginating furrows during prophase and metaphase. We show that the Nuf centrosomal protein is required for recruiting the membrane-associated protein Dah to the furrows. In nuf-mutant embryos, much of the Dah does not reach the furrows and remains in a punctate distribution. This suggests that Dah is recruited to the furrows in vesicles and that the recruiting step is disrupted in nuf mutants. These studies lead to a model in which the centrosomes play an important role in the transport of membrane-associated proteins and other components to the developing furrows.
This article has been cited by other articles:
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  J. Cao, R. Albertson, B. Riggs, C. M. Field, and W. Sullivan Nuf, a Rab11 effector, maintains cytokinetic furrow integrity by promoting local actin polymerization J. Cell Biol., July 21, 2008; (2008) jcb.200712036. [Abstract] [Full Text] [PDF]
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R. Albertson, J. Cao, T.-s. Hsieh, and W. Sullivan Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle J. Cell Biol., May 28, 2008; 181(5): 777 - 790. [Abstract] [Full Text] [PDF]
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 B. Riggs, B. Fasulo, A. Royou, S. Mische, J. Cao, T. S. Hays, and W. Sullivan The Concentration of Nuf, a Rab11 Effector, at the Microtubule-organizing Center Is Cell Cycle regulated, Dynein-dependent, and Coincides with Furrow Formation Mol. Biol. Cell, September 1, 2007; 18(9): 3313 - 3322. [Abstract] [Full Text] [PDF]
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 T. Kidd, R. Abu-Shumays, A. Katzen, J. C. Sisson, G. Jimenez, S. Pinchin, W. Sullivan, and D. Ish-Horowicz The {epsilon}-Subunit of Mitochondrial ATP Synthase Is Required for Normal Spindle Orientation During the Drosophila Embryonic Divisions Genetics, June 1, 2005; 170(2): 697 - 708. [Abstract] [Full Text] [PDF]
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E. A. Sevrioukov, N. Moghrabi, M. Kuhn, and H. Kramer A Mutation in dVps28 Reveals a Link between a Subunit of the Endosomal Sorting Complex Required for Transport-I Complex and the Actin Cytoskeleton in Drosophila Mol. Biol. Cell, May 1, 2005; 16(5): 2301 - 2312. [Abstract] [Full Text] [PDF]
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G. M. Wilson, A. B. Fielding, G. C. Simon, X. Yu, P. D. Andrews, R. S. Hames, A. M. Frey, A. A. Peden, G. W. Gould, and R. Prekeris The FIP3-Rab11 Protein Complex Regulates Recycling Endosome Targeting to the Cleavage Furrow during Late Cytokinesis Mol. Biol. Cell, February 1, 2005; 16(2): 849 - 860. [Abstract] [Full Text] [PDF]
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 T. Lecuit Junctions and vesicular trafficking during Drosophila cellularization J. Cell Sci., July 15, 2004; 117(16): 3427 - 3433. [Abstract] [Full Text] [PDF]
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B. Riggs, W. Rothwell, S. Mische, G. R.X. Hickson, J. Matheson, T. S. Hays, G. W. Gould, and W. Sullivan Actin cytoskeleton remodeling during early Drosophila furrow formation requires recycling endosomal components Nuclear-fallout and Rab11 J. Cell Biol., October 13, 2003; 163(1): 143 - 154. [Abstract] [Full Text] [PDF]
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G. R.X. Hickson, J. Matheson, B. Riggs, V. H. Maier, A. B. Fielding, R. Prekeris, W. Sullivan, F. A. Barr, and G. W. Gould Arfophilins Are Dual Arf/Rab 11 Binding Proteins That Regulate Recycling Endosome Distribution and Are Related to Drosophila Nuclear Fallout Mol. Biol. Cell, July 1, 2003; 14(7): 2908 - 2920. [Abstract] [Full Text] [PDF]
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T. L. Megraw, S. Kilaru, F. R. Turner, and T. C. Kaufman The centrosome is a dynamic structure that ejects PCM flares J. Cell Sci., January 12, 2002; 115(23): 4707 - 4718. [Abstract] [Full Text] [PDF]
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J. C. Sisson, C. Field, R. Ventura, A. Royou, and W. Sullivan Lava Lamp, a Novel Peripheral Golgi Protein, Is Required for Drosophila melanogaster Cellularization J. Cell Biol., November 13, 2000; 151(4): 905 - 918. [Abstract] [Full Text] [PDF]
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C. X. Zhang, W. F. Rothwell, W. Sullivan, and T.-s. Hsieh Discontinuous Actin Hexagon, a Protein Essential for Cortical Furrow Formation in Drosophila, Is Membrane Associated and Hyperphosphorylated Mol. Biol. Cell, March 1, 2000; 11(3): 1011 - 1022. [Abstract] [Full Text]
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