spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lingnau, A.
Right arrow Articles by Russell, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lingnau, A.
Right arrow Articles by Russell, D. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 112, Issue 18 3061-3070, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

Characterization of tGLP-1, a Golgi and lysosome-associated, transmembrane glycoprotein of African trypanosomes

A Lingnau, R Zufferey, M Lingnau and DG Russell
Washington University School of Medicine, Department of Molecular Microbiology, St. Louis, MO 63110, USA.

Purification of endosomal/lysosomal vesicles of Trypanosoma brucei brucei bloodstream forms and generation of monoclonal antibodies led to the isolation of antibodies directed against an 85 kDa, Golgi and endocytic traffic-associated protein termed tGLP-1, Trypanosoma Golgi/lysosome protein-1. Preliminary immunoelectron microscopical analysis revealed that the protein is present in, but not restricted to, the limiting membrane of multivesicular lysosomes and is more abundant in bloodstream forms compared to the procyclic stage. The corresponding gene was cloned and is present as a single copy. Blast searches did not reveal any homologies to other proteins and genes published. The nucleotide sequence of the gene (1848 base pairs) predicted a type 1 membrane topology with an N-terminal signal sequence (20 aa), a luminal domain with 2 N-glycosylation sites (524 aa), a transmembrane domain (23 aa), and a long cytosolic tail domain (49 aa). Polyclonal antibodies raised against the cytosolic tail confirmed the localization of the gene product to multivesicular lysosomes but revealed that the majority of the protein was in the Golgi apparatus. Colabelling with an antibody against p67, a lysosomal glycoprotein of trypanosomes, revealed extensive overlap between the proteins with opposing relative abundance. Expression of the tGLP-1 open reading frame in Leishmania resulted in Golgi localization, and in Toxoplasma, in localization to both the Golgi and endoplasmic reticulum. These data indicate conservation in the functionality of the Golgi-targeting sequence of tGLP-1.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
D. L. Alexander, K. J. Schwartz, A. E. Balber, and J. D. Bangs
Developmentally regulated trafficking of the lysosomal membrane protein p67 in Trypanosoma brucei
J. Cell Sci., August 15, 2002; 115(16): 3253 - 3263.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. R. Roper, M. L. S. Guther, K. G. Milne, and M. A. J. Ferguson
Galactose metabolism is essential for the African sleeping sickness parasite Trypanosoma brucei
PNAS, April 30, 2002; 99(9): 5884 - 5889.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
M. J. McConville, K. A. Mullin, S. C. Ilgoutz, and R. D. Teasdale
Secretory Pathway of Trypanosomatid Parasites
Microbiol. Mol. Biol. Rev., March 1, 2002; 66(1): 122 - 154.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Yang, D. G. Russell, B. Zheng, M. Eiki, and M. G.-S. Lee
Sequence Requirements for Trafficking of the CRAM Transmembrane Protein to the Flagellar Pocket of African Trypanosomes
Mol. Cell. Biol., July 15, 2000; 20(14): 5149 - 5163.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. Dutoya, S. Gibert, G. Lemercier, X. Santarelli, D. Baltz, T. Baltz, and N. Bakalara
A Novel C-terminal Kinesin Is Essential for Maintaining Functional Acidocalcisomes in Trypanosoma brucei
J. Biol. Chem., December 21, 2001; 276(52): 49117 - 49124.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1999