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Journal of Cell Science, Vol 112, Issue 18 3103-3114, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
N Beltraminelli, M Murone and V Simanis
Cell Cycle Control Laboratory, ISREC, Chemin des Boveresses, 1066 Epalinges, Switzerland.
Schizosaccharomyces pombe cdc16p is required to limit the cell to forming a single division septum per cell cycle; the heat-sensitive loss-of-function mutant cdc16-116 completes mitosis, and then undergoes multiple rounds of septum formation without cell cleavage. cdc16p is a homologue of Saccharomyces cerevisiae BUB2p, and has also been implicated in the spindle assembly checkpoint function in S. pombe. To identify other proteins involved in regulating septum formation, we have screened for multicopy suppressors of the cdc16-116 mutation. In this paper, we describe one of these suppressors, zfs1. The null allele (zfs1-D1) is viable. However, at low temperatures it divides at a reduced size, while at higher temperatures, it partially suppresses heat sensitive mutants in genes signalling the onset of septum formation. Zfs1-D1 cells show an increased rate of chromosome loss during exponential growth. Moreover, if assembly of the spindle is prevented, zfs1-D1 cells do not arrest normally, but the activity of cdc2p kinase decays, and cells form a division septum without completing a normal mitosis. We conclude that zfs1 function is required to prevent septum formation and exit from mitosis if the mitotic spindle is not assembled. The suppression of cdc16-116 by zfs1 is independent of dma1 function and the spindle assembly checkpoint genes mad2 and mph1. The genetic interactions of zfs1 with genes regulating septum formation suggest that it may be a modulator of the signal transduction network controlling the onset of septum formation and exit from mitosis.
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  B. J. Cuthbertson, Y. Liao, L. Birnbaumer, and P. J. Blackshear Characterization of zfs1 as an mRNA-binding and -destabilizing Protein in Schizosaccharomyces pombe J. Biol. Chem., February 1, 2008; 283(5): 2586 - 2594. [Abstract] [Full Text] [PDF]
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 A. Grallert, A. Krapp, S. Bagley, V. Simanis, and I. M. Hagan Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity Genes & Dev., May 1, 2004; 18(9): 1007 - 1021. [Abstract] [Full Text] [PDF]
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A. Pidoux, S Uzawa, P. Perry, W. Cande, and R. Allshire Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast J. Cell Sci., January 12, 2000; 113(23): 4177 - 4191. [Abstract] [PDF]
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H. Yamada, S Matsumoto, and T Matsumoto High dosage expression of a zinc finger protein, Grt1, suppresses a mutant of fission yeast slp1(+), a homolog of CDC20/p55CDC/Fizzy J. Cell Sci., January 11, 2000; 113(22): 3989 - 3999. [Abstract] [PDF]
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M. Balasubramanian, D McCollum, and U Surana Tying the knot: linking cytokinesis to the nuclear cycle J. Cell Sci., January 5, 2000; 113(9): 1503 - 1513. [Abstract] [PDF]
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E Salimova, M Sohrmann, N Fournier, and V Simanis The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation J. Cell Sci., January 5, 2000; 113(10): 1695 - 1704. [Abstract] [PDF]
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