|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 20 3433-3441, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
F Echtermeyer, PC Baciu, S Saoncella, Y Ge and PF Goetinck
Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Building 149, Charlestown, Massachusetts 02129, USA. paul.goetinck@cbrc2.mgh.harvard.edu
The formation of focal adhesions and actin stress fibers on fibronectin is dependent on signaling through (&bgr;)1 integrins and the heparan sulfate proteoglycan syndecan-4, and we have analyzed the requirement of the glycosaminoglycan chains of syndecan-4 during these events. Chinese hamster ovary cells with mutations in key enzymes of the glycanation process do not synthesize glycosaminoglycan chains and are unable to assemble actin stress fibers and focal contacts when cultured on fibronectin. Transfection of the mutant cells with a cDNA that encodes the core protein of chicken syndecan-4 leads to the production of unglycanated core protein. The overexpression of syndecan-4 core protein in these mutant cells increases cell spreading and is sufficient for these cells to assemble actin stress fibers and focal adhesions similar to wild-type cells seeded on fibronectin and vitronectin matrices. Syndecan-4 core protein colocalizes to focal contacts in mutant cells that have been transfected with the syndecan-4 core protein cDNA. These data indicate an essential role for the core protein of syndecan-4 in the generation of signals leading to actin stress fiber and focal contact assembly.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Scarpellini, R. Germack, H. Lortat-Jacob, T. Muramatsu, E. Billett, T. Johnson, and E. A. M. Verderio Heparan Sulfate Proteoglycans Are Receptors for the Cell-surface Trafficking and Biological Activity of Transglutaminase-2 J. Biol. Chem., July 3, 2009; 284(27): 18411 - 18423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Choi, E. Lee, S. Kwon, H. Park, J. Y. Yi, S. Kim, I.-O. Han, Y. Yun, and E.-S. Oh Transmembrane Domain-induced Oligomerization Is Crucial for the Functions of Syndecan-2 and Syndecan-4 J. Biol. Chem., December 30, 2005; 280(52): 42573 - 42579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schmidt, F. Echtermeyer, A. Alozie, K. Brands, and E. Buddecke Plasmin- and Thrombin-accelerated Shedding of Syndecan-4 Ectodomain Generates Cleavage Sites at Lys114-Arg115 and Lys129-Val130 Bonds J. Biol. Chem., October 14, 2005; 280(41): 34441 - 34446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Houston, M. A. Julien, S. Parthasarathy, and E. L. Chaikof Oxidized linoleic acid regulates expression and shedding of syndecan-4 Am J Physiol Cell Physiol, February 1, 2005; 288(2): C458 - C466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chen, C. Klass, and A. Woods Syndecan-2 Regulates Transforming Growth Factor-{beta} Signaling J. Biol. Chem., April 16, 2004; 279(16): 15715 - 15718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Mostafavi-Pour, J. A. Askari, S. J. Parkinson, P. J. Parker, T. T.C. Ng, and M. J. Humphries Integrin-specific signaling pathways controlling focal adhesion formation and cell migration J. Cell Biol., April 14, 2003; 161(1): 155 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-T. Lim, R. L. Longley, J. R. Couchman, and A. Woods Direct Binding of Syndecan-4 Cytoplasmic Domain to the Catalytic Domain of Protein Kinase Calpha (PKCalpha ) Increases Focal Adhesion Localization of PKCalpha J. Biol. Chem., April 11, 2003; 278(16): 13795 - 13802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Thodeti, R. Albrechtsen, M. Grauslund, M. Asmar, C. Larsson, Y. Takada, A. M. Mercurio, J. R. Couchman, and U. M. Wewer ADAM12/Syndecan-4 Signaling Promotes beta 1 Integrin-dependent Cell Spreading through Protein Kinase Calpha and RhoA J. Biol. Chem., March 7, 2003; 278(11): 9576 - 9584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Greene, S. Tumova, J. R. Couchman, and A. Woods Syndecan-4 Associates with alpha -Actinin J. Biol. Chem., February 21, 2003; 278(9): 7617 - 7623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Wilcox-Adelman, F. Denhez, and P. F. Goetinck Syndecan-4 Modulates Focal Adhesion Kinase Phosphorylation J. Biol. Chem., August 30, 2002; 277(36): 32970 - 32977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Li and E. L. Chaikof Mechanical Stress Regulates Syndecan-4 Expression and Redistribution in Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., January 1, 2002; 22(1): 61 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. C. Kaneider, C. M. Reinisch, S. Dunzendorfer, J. Romisch, and C. J. Wiederman Syndecan-4 mediates antithrombin-induced chemotaxis of human peripheral blood lymphocytes and monocytes J. Cell Sci., January 1, 2002; 115(1): 227 - 236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. P. Moiseeva Adhesion receptors of vascular smooth muscle cells and their functions Cardiovasc Res, December 1, 2001; 52(3): 372 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Dudek and J. G. N. Garcia Cytoskeletal regulation of pulmonary vascular permeability J Appl Physiol, October 1, 2001; 91(4): 1487 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Iba, R. Albrechtsen, B. Gilpin, C. Frohlich, F. Loechel, A. Zolkiewska, K. Ishiguro, T. Kojima, W. Liu, J. K. Langford, et al. The Cysteine-rich Domain of Human ADAM 12 Supports Cell Adhesion Through Syndecans and Triggers Signaling Events that Lead to {beta}1 Integrin-dependent Cell Spreading J. Cell Biol., May 29, 2000; 149(5): 1143 - 1156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tumova, A. Woods, and J. R. Couchman Heparan Sulfate Chains from Glypican and Syndecans Bind the Hep II Domain of Fibronectin Similarly Despite Minor Structural Differences J. Biol. Chem., March 24, 2000; 275(13): 9410 - 9417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Baciu, S Saoncella, S. Lee, F Denhez, D Leuthardt, and P. Goetinck Syndesmos, a protein that interacts with the cytoplasmic domain of syndecan-4, mediates cell spreading and actin cytoskeletal organization J. Cell Sci., January 1, 2000; 113(2): 315 - 324. [Abstract] [PDF]
|
|
|
|
|
|
J. Couchman and A Woods Syndecan-4 and integrins: combinatorial signaling in cell adhesion J. Cell Sci., January 10, 1999; 112(20): 3415 - 3420. [Abstract] [PDF]
|
|
|
|
|
|
A. Woods and J. R. Couchman Integrin Modulation by Lateral Association J. Biol. Chem., August 4, 2000; 275(32): 24233 - 24236. [Full Text] [PDF]
|
|
