|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 21 3757-3768, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
F Scaerou, I Aguilera, R Saunders, N Kane, L Blottiere and R Karess
C.N.R.S., Centre de Genetique Moleculaire, Avenue de la Terrasse, France.
Mutations in the rough deal (rod) gene of Drosophila greatly increase the missegregation of sister chromatids during mitosis, suggesting a role for this gene product in spindle or kinetochore function. The activity provided by rod also appears to be necessary for the recruitment of two known kinetochore components, Zw10 and cytoplasmic dynein. In this paper we describe the cloning of rough deal and an initial cytological characterization of its product. The Rod protein shares no identifiable structural motif with other known proteins, although apparent homologs exist in the genomes of nematode and man. By immunocytochemistry we show that Rod displays a dynamic intracellular staining pattern, localizing first to kinetochores in prometaphase, but moving to kinetochore microtubules at metaphase. Early in anaphase the protein is once again restricted to the kinetochores, where it persists until the end of telophase. This behavior is in all respects similar to that described for Zw10, and suggests that the proteins function together.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Reis, T. Feijao, S. Gouveia, A. J. Pereira, I. Matos, P. Sampaio, H. Maiato, and C. E. Sunkel Dynein and Mast/Orbit/CLASP have antagonistic roles in regulating kinetochore-microtubule plus-end dynamics J. Cell Sci., July 15, 2009; 122(14): 2543 - 2553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wainman, J. Creque, B. Williams, E. V. Williams, S. Bonaccorsi, M. Gatti, and M. L. Goldberg Roles of the Drosophila NudE protein in kinetochore function and centrosome migration J. Cell Sci., June 1, 2009; 122(11): 1747 - 1758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Erhardt, B. G. Mellone, C. M. Betts, W. Zhang, G. H. Karpen, and A. F. Straight Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation J. Cell Biol., December 1, 2008; 183(5): 805 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Gassmann, A. Essex, J.-S. Hu, P. S. Maddox, F. Motegi, A. Sugimoto, S. M. O'Rourke, B. Bowerman, I. McLeod, J. R. Yates III, et al. A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex Genes & Dev., September 1, 2008; 22(17): 2385 - 2399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Famulski, L. Vos, X. Sun, and G. Chan Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint J. Cell Biol., February 6, 2008; 180(3): 507 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Griffis, N. Stuurman, and R. D. Vale Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore J. Cell Biol., July 30, 2007; 177(6): 1005 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Basto, F. Gergely, V. M. Draviam, H. Ohkura, K. Liley, and J. W. Raff Hsp90 is required to localise cyclin B and Msps/ch-TOG to the mitotic spindle in Drosophila and humans J. Cell Sci., April 1, 2007; 120(7): 1278 - 1287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Zhang, S. Yin, M.-X. Jiang, W. Ma, Y. Hou, C.-G. Liang, L.-Z. Yu, W.-H. Wang, and Q.-Y. Sun Cytoplasmic dynein participates in meiotic checkpoint inactivation in mouse oocytes by transporting cytoplasmic mitotic arrest-deficient (Mad) proteins from kinetochores to spindle poles Reproduction, April 1, 2007; 133(4): 685 - 695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Shaffer, G. Cenci, B. Thompson, G. E. Stephens, E. E. Slawson, K. Adu-Wusu, M. Gatti, and S. C. R. Elgin The Large Isoform of Drosophila melanogaster Heterochromatin Protein 2 Plays a Critical Role in Gene Silencing and Chromosome Structure Genetics, November 1, 2006; 174(3): 1189 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Varma, D. L. Dujardin, S. A. Stehman, and R. B. Vallee Role of the kinetochore/cell cycle checkpoint protein ZW10 in interphase cytoplasmic dynein function J. Cell Biol., February 27, 2006; 172(5): 655 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. H. Siller, M. Serr, R. Steward, T. S. Hays, and C. Q. Doe Live Imaging of Drosophila Brain Neuroblasts Reveals a Role for Lis1/Dynactin in Spindle Assembly and Mitotic Checkpoint Control Mol. Biol. Cell, November 1, 2005; 16(11): 5127 - 5140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Dzhindzhev, S. L. Rogers, R. D. Vale, and H. Ohkura Distinct mechanisms govern the localisation of Drosophila CLIP-190 to unattached kinetochores and microtubule plus-ends J. Cell Sci., August 15, 2005; 118(16): 3781 - 3790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M.J. Hut, H. H. Kampinga, and O. C.M. Sibon Hsp70 Protects Mitotic Cells against Heat-induced Centrosome Damage and Division Abnormalities Mol. Biol. Cell, August 1, 2005; 16(8): 3776 - 3785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J.P.L. Kops, Y. Kim, B. A.A. Weaver, Y. Mao, I. McLeod, J. R. Yates III, M. Tagaya, and D. W. Cleveland ZW10 links mitotic checkpoint signaling to the structural kinetochore J. Cell Biol., April 11, 2005; 169(1): 49 - 60. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Wang, J. M. Cummins, D. Shen, D. P. Cahill, P. V. Jallepalli, T.-L. Wang, D. W. Parsons, G. Traverso, M. Awad, N. Silliman, et al. Three Classes of Genes Mutated In Colorectal Cancers with Chromosomal Instability Cancer Res., May 1, 2004; 64(9): 2998 - 3001. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Pile, P. T. Spellman, R. J. Katzenberger, and D. A. Wassarman The SIN3 Deacetylase Complex Represses Genes Encoding Mitochondrial Proteins: IMPLICATIONS FOR THE REGULATION OF ENERGY METABOLISM J. Biol. Chem., September 26, 2003; 278(39): 37840 - 37848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Williams, Z. Li, S. Liu, E. V. Williams, G. Leung, T. J. Yen, and M. L. Goldberg Zwilch, a New Component of the ZW10/ROD Complex Required for Kinetochore Functions Mol. Biol. Cell, April 1, 2003; 14(4): 1379 - 1391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Scaerou, D. A. Starr, F. Piano, O. Papoulas, R. E. Karess, and M. L. Goldberg The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore J. Cell Sci., January 9, 2001; 114(17): 3103 - 3114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Starr, R Saffery, Z Li, A. Simpson, K. Choo, T. Yen, and M. Goldberg HZwint-1, a novel human kinetochore component that interacts with HZW10 J. Cell Sci., January 6, 2000; 113(11): 1939 - 1950. [Abstract] [PDF]
|
|
|
|
|
|
B.J. Howell, B.F. McEwen, J.C. Canman, D.B. Hoffman, E.M. Farrar, C.L. Rieder, and E.D. Salmon Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation J. Cell Biol., December 24, 2001; 155(7): 1159 - 1172. [Abstract] [Full Text] [PDF]
|
|
