|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 22 4041-4050, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
J Matuliene, R Essner, J Ryu, Y Hamaguchi, PW Baas, T Haraguchi, Y Hiraoka and R Kuriyama
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN55455, USA.
CHO2 is a mammalian minus-end-directed kinesin-like motor protein present in interphase centrosomes/nuclei and mitotic spindle fibers/poles. Expression of HA- or GFP-tagged subfragments in transfected CHO cells revealed the presence of the nuclear localization site at the N-terminal tail. This domain becomes associated with spindle fibers during mitosis, indicating that the tail is capable of interaction with microtubules in vivo. While the central stalk diffusely distributes in the entire cytoplasm of cells, the motor domain co-localizes with microtubules throughout the cell cycle, which is eliminated by mutation of the ATP-binding consensus motif from GKT to AAA. Overexpression of the full-length CHO2 causes mitotic arrest and spindle abnormality. The effect of protein expression was first seen around the polar region where microtubule tended to be bundled together. A higher level of protein expression induces more elongated spindles which eventually become disorganized by loosing the structural integrity between microtubule bundles. Live cell observation demonstrated that GFP-labeled microtubule bundles underwent continuous changes in their relative position to one another through repeated attachment and detachment at one end; this results in the formation of irregular number of microtubule focal points in mitotic arrested cells. Thus the primary action of CHO2 appears to cross-link microtubules and move toward the minus-end direction to maintain association of the microtubule end at the pole. In contrast to the full-length of CHO2, overexpression of neither truncated nor mutant polypeptides resulted in significant effects on mitosis and mitotic spindles, suggesting that the function of CHO2 in mammalian cells may be redundant with other motor molecules during cell division.
This article has been cited by other articles:
|
|
 |
|
  A. Oladipo, A. Cowan, and V. Rodionov Microtubule Motor Ncd Induces Sliding of Microtubules In Vivo Mol. Biol. Cell, September 1, 2007; 18(9): 3601 - 3606. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Christodoulou, C. W. Lederer, T. Surrey, I. Vernos, and N. Santama Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication J. Cell Sci., May 15, 2006; 119(10): 2035 - 2047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Ambrose, W. Li, A. Marcus, H. Ma, and R. Cyr A Minus-End-directed Kinesin with Plus-End Tracking Protein Activity Is Involved in Spindle Morphogenesis Mol. Biol. Cell, April 1, 2005; 16(4): 1584 - 1592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chakravarty, L. Howard, and D. A. Compton A Mechanistic Model for the Organization of Microtubule Asters by Motor and Non-Motor Proteins in a Mammalian Mitotic Extract Mol. Biol. Cell, May 1, 2004; 15(5): 2116 - 2132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Ems-McClung, Y. Zheng, and C. E. Walczak Importin {alpha}/{beta} and Ran-GTP Regulate XCTK2 Microtubule Binding through a Bipartite Nuclear Localization Signal Mol. Biol. Cell, January 1, 2004; 15(1): 46 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Matuliene and R. Kuriyama Kinesin-like Protein CHO1 Is Required for the Formation of Midbody Matrix and the Completion of Cytokinesis in Mammalian Cells Mol. Biol. Cell, June 1, 2002; 13(6): 1832 - 1845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Gordon, L. Howard, and D. A. Compton Chromosome Movement in Mitosis Requires Microtubule Anchorage at Spindle Poles J. Cell Biol., January 29, 2001; 152(3): 425 - 434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ohta, R. Essner, J.-H. Ryu, R. E. Palazzo, Y. Uetake, and R. Kuriyama Characterization of Cep135, a novel coiled-coil centrosomal protein involved in microtubule organization in mammalian cells J. Cell Biol., January 7, 2002; 156(1): 87 - 100. [Abstract] [Full Text] [PDF]
|
|
